Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure
Abstract: Background: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short-and medium-term PM10 exposure. Methods: Whole genome gene expression was measured in peripheral blood of...
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ftunivantwerpen:c:irua:145713 2023-07-16T03:57:58+02:00 Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure Winckelmans, Ellen Nawrot, Tim S. Tsamou, Maria Den Hond, Elly Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Van Larebeke, Nicolas Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte de Kok, Theo M. Vrijens, Karen 2017 pdf https://hdl.handle.net/10067/1457130151162165141 https://repository.uantwerpen.be/docman/irua/e3730f/145713.pdf eng eng info:eu-repo/semantics/altIdentifier/doi/10.1186/S12940-017-0292-7 info:eu-repo/semantics/altIdentifier/isi/000408044200001 info:eu-repo/semantics/openAccess 1476-069X Environmental health: a global access science source Chemistry Biology Human medicine info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2017 ftunivantwerpen https://doi.org/10.1186/S12940-017-0292-7 2023-06-26T22:23:25Z Abstract: Background: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short-and medium-term PM10 exposure. Methods: Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results: Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions: In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. Article in Journal/Newspaper Carbonic acid IRUA - Institutional Repository van de Universiteit Antwerpen Environmental Health 16 1 |
institution |
Open Polar |
collection |
IRUA - Institutional Repository van de Universiteit Antwerpen |
op_collection_id |
ftunivantwerpen |
language |
English |
topic |
Chemistry Biology Human medicine |
spellingShingle |
Chemistry Biology Human medicine Winckelmans, Ellen Nawrot, Tim S. Tsamou, Maria Den Hond, Elly Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Van Larebeke, Nicolas Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte de Kok, Theo M. Vrijens, Karen Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
topic_facet |
Chemistry Biology Human medicine |
description |
Abstract: Background: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short-and medium-term PM10 exposure. Methods: Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results: Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions: In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. |
format |
Article in Journal/Newspaper |
author |
Winckelmans, Ellen Nawrot, Tim S. Tsamou, Maria Den Hond, Elly Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Van Larebeke, Nicolas Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte de Kok, Theo M. Vrijens, Karen |
author_facet |
Winckelmans, Ellen Nawrot, Tim S. Tsamou, Maria Den Hond, Elly Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Van Larebeke, Nicolas Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte de Kok, Theo M. Vrijens, Karen |
author_sort |
Winckelmans, Ellen |
title |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_short |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_fullStr |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full_unstemmed |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_sort |
transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
publishDate |
2017 |
url |
https://hdl.handle.net/10067/1457130151162165141 https://repository.uantwerpen.be/docman/irua/e3730f/145713.pdf |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
1476-069X Environmental health: a global access science source |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/S12940-017-0292-7 info:eu-repo/semantics/altIdentifier/isi/000408044200001 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/S12940-017-0292-7 |
container_title |
Environmental Health |
container_volume |
16 |
container_issue |
1 |
_version_ |
1771544880979378176 |