Molecular response of **Sargassum vulgare** to acidification at volcanic CO_{2}$ vents : insights from de novo transcriptomic analysis
Abstract: Ocean acidification is an emerging problem that is expected to impact ocean species to varying degrees. Currently, little is known about its effect on molecular mechanisms induced in fleshy macroalgae. To elucidate genome wide responses to acidification, a transcriptome analysis was carrie...
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ftunivantwerpen:c:irua:141408 2023-07-16T04:00:19+02:00 Molecular response of **Sargassum vulgare** to acidification at volcanic CO_{2}$ vents : insights from de novo transcriptomic analysis Kumar, Amit Castellano, Immacolata Patti, Francesco Paulo Delledonne, Massimo Abd Elgawad, Hamada Beemster, Gerrit Asard, Han Palumbo, Anna Buia, Maria Cristina 2017 pdf https://hdl.handle.net/10067/1414080151162165141 https://repository.uantwerpen.be/docman/irua/68875a/141408_2018_03_23.pdf eng eng info:eu-repo/semantics/altIdentifier/doi/10.1111/MEC.14034 info:eu-repo/semantics/altIdentifier/isi/000400334600006 info:eu-repo/semantics/openAccess 0962-1083 Molecular ecology Chemistry Biology info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/article 2017 ftunivantwerpen https://doi.org/10.1111/MEC.14034 2023-06-26T22:21:50Z Abstract: Ocean acidification is an emerging problem that is expected to impact ocean species to varying degrees. Currently, little is known about its effect on molecular mechanisms induced in fleshy macroalgae. To elucidate genome wide responses to acidification, a transcriptome analysis was carried out on Sargassum vulgare populations growing under acidified conditions at volcanic CO2 vents and compared with populations in a control site. Several transcripts involved in a wide range of cellular and metabolic processes were differentially expressed. No drastic changes were observed in the carbon acquisition processes and RuBisCO level. Moreover, relatively few stress genes, including those for antioxidant enzymes and heat-shock proteins, were affected. Instead, increased expression of transcripts involved in energy metabolism, photosynthetic processes and ion homeostasis suggested that algae increased energy production to maintain ion homeostasis and other cellular processes. Also, an increased allocation of carbon to cell wall and carbon storage was observed. A number of genes encoding proteins involved in cellular signalling, information storage and processing and transposition were differentially expressed between the two conditions. The transcriptional changes of key enzymes were largely confirmed by enzymatic activity measurements. Altogether, the changes induced by acidification indicate an adaptation of growth and development of S. vulgare at the volcanic CO2 vents, suggesting that this fleshy alga exhibits a high plasticity to low pH and can adopt molecular strategies to grow also in future more acidified waters. Article in Journal/Newspaper Ocean acidification IRUA - Institutional Repository van de Universiteit Antwerpen Molecular Ecology 26 8 2276 2290 |
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IRUA - Institutional Repository van de Universiteit Antwerpen |
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ftunivantwerpen |
language |
English |
topic |
Chemistry Biology |
spellingShingle |
Chemistry Biology Kumar, Amit Castellano, Immacolata Patti, Francesco Paulo Delledonne, Massimo Abd Elgawad, Hamada Beemster, Gerrit Asard, Han Palumbo, Anna Buia, Maria Cristina Molecular response of **Sargassum vulgare** to acidification at volcanic CO_{2}$ vents : insights from de novo transcriptomic analysis |
topic_facet |
Chemistry Biology |
description |
Abstract: Ocean acidification is an emerging problem that is expected to impact ocean species to varying degrees. Currently, little is known about its effect on molecular mechanisms induced in fleshy macroalgae. To elucidate genome wide responses to acidification, a transcriptome analysis was carried out on Sargassum vulgare populations growing under acidified conditions at volcanic CO2 vents and compared with populations in a control site. Several transcripts involved in a wide range of cellular and metabolic processes were differentially expressed. No drastic changes were observed in the carbon acquisition processes and RuBisCO level. Moreover, relatively few stress genes, including those for antioxidant enzymes and heat-shock proteins, were affected. Instead, increased expression of transcripts involved in energy metabolism, photosynthetic processes and ion homeostasis suggested that algae increased energy production to maintain ion homeostasis and other cellular processes. Also, an increased allocation of carbon to cell wall and carbon storage was observed. A number of genes encoding proteins involved in cellular signalling, information storage and processing and transposition were differentially expressed between the two conditions. The transcriptional changes of key enzymes were largely confirmed by enzymatic activity measurements. Altogether, the changes induced by acidification indicate an adaptation of growth and development of S. vulgare at the volcanic CO2 vents, suggesting that this fleshy alga exhibits a high plasticity to low pH and can adopt molecular strategies to grow also in future more acidified waters. |
format |
Article in Journal/Newspaper |
author |
Kumar, Amit Castellano, Immacolata Patti, Francesco Paulo Delledonne, Massimo Abd Elgawad, Hamada Beemster, Gerrit Asard, Han Palumbo, Anna Buia, Maria Cristina |
author_facet |
Kumar, Amit Castellano, Immacolata Patti, Francesco Paulo Delledonne, Massimo Abd Elgawad, Hamada Beemster, Gerrit Asard, Han Palumbo, Anna Buia, Maria Cristina |
author_sort |
Kumar, Amit |
title |
Molecular response of **Sargassum vulgare** to acidification at volcanic CO_{2}$ vents : insights from de novo transcriptomic analysis |
title_short |
Molecular response of **Sargassum vulgare** to acidification at volcanic CO_{2}$ vents : insights from de novo transcriptomic analysis |
title_full |
Molecular response of **Sargassum vulgare** to acidification at volcanic CO_{2}$ vents : insights from de novo transcriptomic analysis |
title_fullStr |
Molecular response of **Sargassum vulgare** to acidification at volcanic CO_{2}$ vents : insights from de novo transcriptomic analysis |
title_full_unstemmed |
Molecular response of **Sargassum vulgare** to acidification at volcanic CO_{2}$ vents : insights from de novo transcriptomic analysis |
title_sort |
molecular response of **sargassum vulgare** to acidification at volcanic co_{2}$ vents : insights from de novo transcriptomic analysis |
publishDate |
2017 |
url |
https://hdl.handle.net/10067/1414080151162165141 https://repository.uantwerpen.be/docman/irua/68875a/141408_2018_03_23.pdf |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
0962-1083 Molecular ecology |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/MEC.14034 info:eu-repo/semantics/altIdentifier/isi/000400334600006 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/MEC.14034 |
container_title |
Molecular Ecology |
container_volume |
26 |
container_issue |
8 |
container_start_page |
2276 |
op_container_end_page |
2290 |
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1771548973162561536 |