In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome
Abstract: Through bacterial cloning, a non-specific product co-amplified in a previous whole mitochondrial genome study of Canis lupus familiaris was identified as part of a Numt on chromosome 29 of the dog. Even though further analysis confirmed the fidelity of the mitochondrial genome sequencing r...
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Language: | English |
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Online Access: | https://hdl.handle.net/10067/1270110151162165141 |
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ftunivantwerpen:c:irua:127011 2023-07-16T03:57:53+02:00 In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome Verscheure, Sophie Backeljau, Thierry Desmyter, Stijn 2015 https://hdl.handle.net/10067/1270110151162165141 eng eng info:eu-repo/semantics/altIdentifier/doi/10.1007/S10709-015-9844-3 info:eu-repo/semantics/altIdentifier/isi/000357044300007 info:eu-repo/semantics/closedAccess 0016-6707 Genetica Human medicine info:eu-repo/semantics/article 2015 ftunivantwerpen https://doi.org/10.1007/S10709-015-9844-3 2023-06-26T22:19:07Z Abstract: Through bacterial cloning, a non-specific product co-amplified in a previous whole mitochondrial genome study of Canis lupus familiaris was identified as part of a Numt on chromosome 29 of the dog. Even though further analysis confirmed the fidelity of the mitochondrial genome sequencing results, it still highlighted the risk of Numt contamination. A computer-based search of the dog's nuclear genome for segments homologous to the mtDNA sequence revealed the extent of this risk. Over 150 Numts of various sizes were observed throughout all but two chromosomes, covering all positions of the mtDNA. One of the Numts on chromosome 11 even covered over 95 % of the entire dog mtDNA sequence. This comprehensive list of Numts was provided to assist researchers with the evaluation of dog mtDNA sequencing protocols for Numt co-amplification. Article in Journal/Newspaper Canis lupus IRUA - Institutional Repository van de Universiteit Antwerpen Genetica 143 4 453 458 |
institution |
Open Polar |
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IRUA - Institutional Repository van de Universiteit Antwerpen |
op_collection_id |
ftunivantwerpen |
language |
English |
topic |
Human medicine |
spellingShingle |
Human medicine Verscheure, Sophie Backeljau, Thierry Desmyter, Stijn In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome |
topic_facet |
Human medicine |
description |
Abstract: Through bacterial cloning, a non-specific product co-amplified in a previous whole mitochondrial genome study of Canis lupus familiaris was identified as part of a Numt on chromosome 29 of the dog. Even though further analysis confirmed the fidelity of the mitochondrial genome sequencing results, it still highlighted the risk of Numt contamination. A computer-based search of the dog's nuclear genome for segments homologous to the mtDNA sequence revealed the extent of this risk. Over 150 Numts of various sizes were observed throughout all but two chromosomes, covering all positions of the mtDNA. One of the Numts on chromosome 11 even covered over 95 % of the entire dog mtDNA sequence. This comprehensive list of Numts was provided to assist researchers with the evaluation of dog mtDNA sequencing protocols for Numt co-amplification. |
format |
Article in Journal/Newspaper |
author |
Verscheure, Sophie Backeljau, Thierry Desmyter, Stijn |
author_facet |
Verscheure, Sophie Backeljau, Thierry Desmyter, Stijn |
author_sort |
Verscheure, Sophie |
title |
In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome |
title_short |
In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome |
title_full |
In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome |
title_fullStr |
In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome |
title_full_unstemmed |
In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome |
title_sort |
in silico discovery of a nearly complete mitochondrial genome numt in the dog (canis lupus familiaris) nuclear genome |
publishDate |
2015 |
url |
https://hdl.handle.net/10067/1270110151162165141 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
0016-6707 Genetica |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/S10709-015-9844-3 info:eu-repo/semantics/altIdentifier/isi/000357044300007 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/S10709-015-9844-3 |
container_title |
Genetica |
container_volume |
143 |
container_issue |
4 |
container_start_page |
453 |
op_container_end_page |
458 |
_version_ |
1771544743901134848 |