Identification of neuroendocrine pathways regulating physiological processes in the oyster Crassostrea gigas via the functional characterization of ligand / receptor couples
The neuroendocrine regulators of the physiology of Lophotrochozoa, the sister clade of Ecdysozoa among Protostoma, remain poorly understood. Thanks to the recent emergence of genomic, transcriptomic and peptidomic resources in the Pacific oyster Crassostrea gigas, the functional characterization of...
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Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
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2019
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Online Access: | https://theses.hal.science/tel-02299481 https://theses.hal.science/tel-02299481/document https://theses.hal.science/tel-02299481/file/sygal_fusion_28103-schwartz-julie.pdf |
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ftunivantilles:oai:HAL:tel-02299481v1 2024-04-14T08:10:34+00:00 Identification of neuroendocrine pathways regulating physiological processes in the oyster Crassostrea gigas via the functional characterization of ligand / receptor couples Identification de voies neuroendocriniennes du contrôle de la physiologie chez l'huître Crassostrea gigas par la caractérisation fonctionnelle de couples ligands/récepteurs Schwartz, Julie Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Normandie Université Pascal Favrel 2019-01-25 https://theses.hal.science/tel-02299481 https://theses.hal.science/tel-02299481/document https://theses.hal.science/tel-02299481/file/sygal_fusion_28103-schwartz-julie.pdf fr fre HAL CCSD NNT: 2019NORMC203 tel-02299481 https://theses.hal.science/tel-02299481 https://theses.hal.science/tel-02299481/document https://theses.hal.science/tel-02299481/file/sygal_fusion_28103-schwartz-julie.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-02299481 Biologie animale. Normandie Université, 2019. Français. ⟨NNT : 2019NORMC203⟩ G-proteins coupled receptors Physiology Oyster Crassostrea gigas Cholecystokinin Calcitonin Dopamine Huître Crassostrea gigas [SDV.BA]Life Sciences [q-bio]/Animal biology info:eu-repo/semantics/doctoralThesis Theses 2019 ftunivantilles 2024-03-21T17:56:11Z The neuroendocrine regulators of the physiology of Lophotrochozoa, the sister clade of Ecdysozoa among Protostoma, remain poorly understood. Thanks to the recent emergence of genomic, transcriptomic and peptidomic resources in the Pacific oyster Crassostrea gigas, the functional characterization of ligand/receptor pairs regulating a diversity of physiological functions has been facilitated. Using a reverse endocrinology approach, a number of orphan G Protein-Coupled Receptors (GPCRs) have been functionally characterized. Three signalling systems have been studied in the oyster: The cholecystokinin/sulfakinin (CCK/SK), the calcitonin (CT) and the dopamine (DA) signalling systems. Two CCK/SK receptors and ligands have been characterized. In vitro bioassays and feeding conditions suggested the potential involvement of this signalling system in the regulation of digestion and satiety. Besides, two couples of CT-type peptides and receptors have been characterized showing, as for their vertebrate counterparts, their possible role in the regulation of water and ion balance. A receptor specifically activated by dopamine and by tyramine has also been characterized. This signalling system appeared to be implicated in the mediation of stress and to play a role in the regulatory processes of reproduction in the gonads. This study allowed the characterization in the oyster of ligand receptor pairs homolog to known signalling systems present in Ecdysozoa and vertebrates, thus confirming the origin of these neuroendocrine systems in the common ancestor of Bilateria. The results of this study also contributed to understand how the oyster integrates external parameters and adapts to various environmental constrains. Les acteurs neuroendocriniens régulant la physiologie des Lophotrochozoaires, groupe frère des Ecdysozoaires parmi les Protostomiens, demeurent peu connus. Grâce à l’émergence récente de ressources génomiques, transcriptomiques et peptidomiques chez l’huître creuse Crassostrea gigas, l’étude des couples ... Doctoral or Postdoctoral Thesis Crassostrea gigas Pacific oyster Université des Antilles (UAG): HAL Pacific |
institution |
Open Polar |
collection |
Université des Antilles (UAG): HAL |
op_collection_id |
ftunivantilles |
language |
French |
topic |
G-proteins coupled receptors Physiology Oyster Crassostrea gigas Cholecystokinin Calcitonin Dopamine Huître Crassostrea gigas [SDV.BA]Life Sciences [q-bio]/Animal biology |
spellingShingle |
G-proteins coupled receptors Physiology Oyster Crassostrea gigas Cholecystokinin Calcitonin Dopamine Huître Crassostrea gigas [SDV.BA]Life Sciences [q-bio]/Animal biology Schwartz, Julie Identification of neuroendocrine pathways regulating physiological processes in the oyster Crassostrea gigas via the functional characterization of ligand / receptor couples |
topic_facet |
G-proteins coupled receptors Physiology Oyster Crassostrea gigas Cholecystokinin Calcitonin Dopamine Huître Crassostrea gigas [SDV.BA]Life Sciences [q-bio]/Animal biology |
description |
The neuroendocrine regulators of the physiology of Lophotrochozoa, the sister clade of Ecdysozoa among Protostoma, remain poorly understood. Thanks to the recent emergence of genomic, transcriptomic and peptidomic resources in the Pacific oyster Crassostrea gigas, the functional characterization of ligand/receptor pairs regulating a diversity of physiological functions has been facilitated. Using a reverse endocrinology approach, a number of orphan G Protein-Coupled Receptors (GPCRs) have been functionally characterized. Three signalling systems have been studied in the oyster: The cholecystokinin/sulfakinin (CCK/SK), the calcitonin (CT) and the dopamine (DA) signalling systems. Two CCK/SK receptors and ligands have been characterized. In vitro bioassays and feeding conditions suggested the potential involvement of this signalling system in the regulation of digestion and satiety. Besides, two couples of CT-type peptides and receptors have been characterized showing, as for their vertebrate counterparts, their possible role in the regulation of water and ion balance. A receptor specifically activated by dopamine and by tyramine has also been characterized. This signalling system appeared to be implicated in the mediation of stress and to play a role in the regulatory processes of reproduction in the gonads. This study allowed the characterization in the oyster of ligand receptor pairs homolog to known signalling systems present in Ecdysozoa and vertebrates, thus confirming the origin of these neuroendocrine systems in the common ancestor of Bilateria. The results of this study also contributed to understand how the oyster integrates external parameters and adapts to various environmental constrains. Les acteurs neuroendocriniens régulant la physiologie des Lophotrochozoaires, groupe frère des Ecdysozoaires parmi les Protostomiens, demeurent peu connus. Grâce à l’émergence récente de ressources génomiques, transcriptomiques et peptidomiques chez l’huître creuse Crassostrea gigas, l’étude des couples ... |
author2 |
Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Normandie Université Pascal Favrel |
format |
Doctoral or Postdoctoral Thesis |
author |
Schwartz, Julie |
author_facet |
Schwartz, Julie |
author_sort |
Schwartz, Julie |
title |
Identification of neuroendocrine pathways regulating physiological processes in the oyster Crassostrea gigas via the functional characterization of ligand / receptor couples |
title_short |
Identification of neuroendocrine pathways regulating physiological processes in the oyster Crassostrea gigas via the functional characterization of ligand / receptor couples |
title_full |
Identification of neuroendocrine pathways regulating physiological processes in the oyster Crassostrea gigas via the functional characterization of ligand / receptor couples |
title_fullStr |
Identification of neuroendocrine pathways regulating physiological processes in the oyster Crassostrea gigas via the functional characterization of ligand / receptor couples |
title_full_unstemmed |
Identification of neuroendocrine pathways regulating physiological processes in the oyster Crassostrea gigas via the functional characterization of ligand / receptor couples |
title_sort |
identification of neuroendocrine pathways regulating physiological processes in the oyster crassostrea gigas via the functional characterization of ligand / receptor couples |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://theses.hal.science/tel-02299481 https://theses.hal.science/tel-02299481/document https://theses.hal.science/tel-02299481/file/sygal_fusion_28103-schwartz-julie.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
https://theses.hal.science/tel-02299481 Biologie animale. Normandie Université, 2019. Français. ⟨NNT : 2019NORMC203⟩ |
op_relation |
NNT: 2019NORMC203 tel-02299481 https://theses.hal.science/tel-02299481 https://theses.hal.science/tel-02299481/document https://theses.hal.science/tel-02299481/file/sygal_fusion_28103-schwartz-julie.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1796308181185462272 |