Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – The pacific oyster (Crassostrea gigas)
International audience The egg-laying hormones (ELHs) of gastropod mollusks were characterized more than forty years ago. Yet, theyhave remained little explored in other mollusks. To gain insights into the functionality of the ELH signalingsystem in a bivalve mollusk – the oyster Crassostrea gigas,...
Published in: | General and Comparative Endocrinology |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2023
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Subjects: | |
Online Access: | https://hal.science/hal-04317342 https://hal.science/hal-04317342/document https://hal.science/hal-04317342/file/GCE-D-23-00260_R1-UM.pdf https://doi.org/10.1016/j.ygcen.2023.114417 |
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ftunivantilles:oai:HAL:hal-04317342v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Université des Antilles (UAG): HAL |
op_collection_id |
ftunivantilles |
language |
English |
topic |
Oyster GPCR Egg-laying hormone Reproduction Stress [SDV]Life Sciences [q-bio] |
spellingShingle |
Oyster GPCR Egg-laying hormone Reproduction Stress [SDV]Life Sciences [q-bio] Favrel, Pascal Dubos, M.P. Bernay, B. Pasquier, J. Schwartz, J. Lefranc, B. Mouret, L. Rivière, G. Leprince, J. Bondon, A. Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – The pacific oyster (Crassostrea gigas) |
topic_facet |
Oyster GPCR Egg-laying hormone Reproduction Stress [SDV]Life Sciences [q-bio] |
description |
International audience The egg-laying hormones (ELHs) of gastropod mollusks were characterized more than forty years ago. Yet, theyhave remained little explored in other mollusks. To gain insights into the functionality of the ELH signalingsystem in a bivalve mollusk – the oyster Crassostrea gigas, this study investigates the processing of its ELH pre-cursor (Cragi-ELH) by mass spectrometry. Some of the ELH mature peptides identified in this study were sub-sequently investigated by nuclear magnetic resonance and shown to adopt an extended alpha-helix structure in amicellar medium mimicking the plasma membrane. To further characterize the ELH signaling system in C. gigas,a G protein-coupled receptor phylogenetically related to ecdysozoan diuretic hormone DH44 and corticotropin-releasing hormone (CRH) receptors named Cragi-ELHR was also characterized functionally and shown to bespecifically activated by the two predicted mature ELH peptides and their N-terminal fragments. Both Cragi-ELHand Cragi-ELHR encoding genes were mostly expressed in the visceral ganglia (VG). Cragi-ELH expression wassignificantly increased in the VG of both fully mature male and female oysters at the spawning stage. When theoysters were submitted to a nutritional or hyposaline stress, no change in the expression of the ligand or receptorgenes was recorded, except for Cragi-ELHR only during a mild acclimation episode to brackish water. Theseresults suggest a role of Cragi-ELH signaling in the regulation of reproduction but not in mediating the stressresponse in our experimental conditions. |
author2 |
Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) Neuroendocrine, Endocrine and Germinal Differentiation Communication (NorDic) Université de Rouen Normandie (UNIROUEN) Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM) Institut des Sciences Chimiques de Rennes (ISCR) Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN) Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB) Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN) Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-High-tech Research Infrastructures for Life Sciences (HeRacLeS) Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Council of the Normandy Region (RIN ECUME: 18E01643-18P02383 |
format |
Article in Journal/Newspaper |
author |
Favrel, Pascal Dubos, M.P. Bernay, B. Pasquier, J. Schwartz, J. Lefranc, B. Mouret, L. Rivière, G. Leprince, J. Bondon, A. |
author_facet |
Favrel, Pascal Dubos, M.P. Bernay, B. Pasquier, J. Schwartz, J. Lefranc, B. Mouret, L. Rivière, G. Leprince, J. Bondon, A. |
author_sort |
Favrel, Pascal |
title |
Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – The pacific oyster (Crassostrea gigas) |
title_short |
Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – The pacific oyster (Crassostrea gigas) |
title_full |
Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – The pacific oyster (Crassostrea gigas) |
title_fullStr |
Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – The pacific oyster (Crassostrea gigas) |
title_full_unstemmed |
Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – The pacific oyster (Crassostrea gigas) |
title_sort |
structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – the pacific oyster (crassostrea gigas) |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://hal.science/hal-04317342 https://hal.science/hal-04317342/document https://hal.science/hal-04317342/file/GCE-D-23-00260_R1-UM.pdf https://doi.org/10.1016/j.ygcen.2023.114417 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.science/hal-04317342 General and Comparative Endocrinology, In press, 346, pp.114417. ⟨10.1016/j.ygcen.2023.114417⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2023.114417 hal-04317342 https://hal.science/hal-04317342 https://hal.science/hal-04317342/document https://hal.science/hal-04317342/file/GCE-D-23-00260_R1-UM.pdf doi:10.1016/j.ygcen.2023.114417 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.ygcen.2023.114417 |
container_title |
General and Comparative Endocrinology |
container_volume |
346 |
container_start_page |
114417 |
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1797581623805345792 |
spelling |
ftunivantilles:oai:HAL:hal-04317342v1 2024-04-28T08:16:32+00:00 Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – The pacific oyster (Crassostrea gigas) Favrel, Pascal Dubos, M.P. Bernay, B. Pasquier, J. Schwartz, J. Lefranc, B. Mouret, L. Rivière, G. Leprince, J. Bondon, A. Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) Neuroendocrine, Endocrine and Germinal Differentiation Communication (NorDic) Université de Rouen Normandie (UNIROUEN) Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM) Institut des Sciences Chimiques de Rennes (ISCR) Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN) Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB) Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN) Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-High-tech Research Infrastructures for Life Sciences (HeRacLeS) Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Council of the Normandy Region (RIN ECUME: 18E01643-18P02383 2023-11-28 https://hal.science/hal-04317342 https://hal.science/hal-04317342/document https://hal.science/hal-04317342/file/GCE-D-23-00260_R1-UM.pdf https://doi.org/10.1016/j.ygcen.2023.114417 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2023.114417 hal-04317342 https://hal.science/hal-04317342 https://hal.science/hal-04317342/document https://hal.science/hal-04317342/file/GCE-D-23-00260_R1-UM.pdf doi:10.1016/j.ygcen.2023.114417 info:eu-repo/semantics/OpenAccess ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.science/hal-04317342 General and Comparative Endocrinology, In press, 346, pp.114417. ⟨10.1016/j.ygcen.2023.114417⟩ Oyster GPCR Egg-laying hormone Reproduction Stress [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2023 ftunivantilles https://doi.org/10.1016/j.ygcen.2023.114417 2024-04-17T23:47:11Z International audience The egg-laying hormones (ELHs) of gastropod mollusks were characterized more than forty years ago. Yet, theyhave remained little explored in other mollusks. To gain insights into the functionality of the ELH signalingsystem in a bivalve mollusk – the oyster Crassostrea gigas, this study investigates the processing of its ELH pre-cursor (Cragi-ELH) by mass spectrometry. Some of the ELH mature peptides identified in this study were sub-sequently investigated by nuclear magnetic resonance and shown to adopt an extended alpha-helix structure in amicellar medium mimicking the plasma membrane. To further characterize the ELH signaling system in C. gigas,a G protein-coupled receptor phylogenetically related to ecdysozoan diuretic hormone DH44 and corticotropin-releasing hormone (CRH) receptors named Cragi-ELHR was also characterized functionally and shown to bespecifically activated by the two predicted mature ELH peptides and their N-terminal fragments. Both Cragi-ELHand Cragi-ELHR encoding genes were mostly expressed in the visceral ganglia (VG). Cragi-ELH expression wassignificantly increased in the VG of both fully mature male and female oysters at the spawning stage. When theoysters were submitted to a nutritional or hyposaline stress, no change in the expression of the ligand or receptorgenes was recorded, except for Cragi-ELHR only during a mild acclimation episode to brackish water. Theseresults suggest a role of Cragi-ELH signaling in the regulation of reproduction but not in mediating the stressresponse in our experimental conditions. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université des Antilles (UAG): HAL General and Comparative Endocrinology 346 114417 |