Virulence of Ostreid herpesvirus 1 μVar in sea water at 16 °C and 25 °C
International audience The Ostreid herpesvirus 1 (OsHV-1) is associated with the mortality of young oysters Crassostrea gigas in France. The virus could infect different marine bivalve species and OsHV-1 DNA was previously detected in crustacean, marine gastropod, and sediment. Nevertheless, the eff...
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ftunivantilles:oai:HAL:hal-03402163v1 2024-04-28T08:16:33+00:00 Virulence of Ostreid herpesvirus 1 μVar in sea water at 16 °C and 25 °C Martenot, Claire Denéchère, Lucie Hubert, Paul Metayer, Laetitia Oden, Elise Trancart, Suzanne Travaillé, Emmanuelle Houssin, Maryline LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) 2015-03 https://normandie-univ.hal.science/hal-03402163 https://doi.org/10.1016/j.aquaculture.2015.01.012 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2015.01.012 hal-03402163 https://normandie-univ.hal.science/hal-03402163 doi:10.1016/j.aquaculture.2015.01.012 ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://normandie-univ.hal.science/hal-03402163 Aquaculture, 2015, 439, pp.1-6. ⟨10.1016/j.aquaculture.2015.01.012⟩ Herpesvirus Real-time RT PCR Crassostrea gigas Injection challenge [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2015 ftunivantilles https://doi.org/10.1016/j.aquaculture.2015.01.012 2024-04-17T23:47:11Z International audience The Ostreid herpesvirus 1 (OsHV-1) is associated with the mortality of young oysters Crassostrea gigas in France. The virus could infect different marine bivalve species and OsHV-1 DNA was previously detected in crustacean, marine gastropod, and sediment. Nevertheless, the effect of the temperature on the stability of infectious viral particles in sea water remains unpublished. During a mortality event, dead oysters may release virus in the sea water such as free viral particles, viral particles included in cells or attached to a support like plankton, suspended particulate matters, and microalgae. Oysters placed around the infectious source may filtrate the virus and then be infected by OsHV-1. In the present study, we investigated the OsHV-1 μVar virulence in sea water at 16 °C and 25 °C. Filtered infected tissue homogenates were kept at 16 °C or 25 °C prior to injection challenge of uninfected spats. A real-time reverse transcription PCR targeting the OsHV-1 polymerase gene has been developed to confirm the death cause oysters collected during the experiment. The RNA detection of herpesvirus in their hosts is a sign of viral replication and indicates that the virus is certainly infectious. Results showed that the OsHV-1 μVar remained infectious and induced mortality after 33 h at 25 °C and 54 h at 16 °C in controlled conditions. The OsHV-1 μVar is thus able to persist in sea water and high temperatures seem to reduce its infectivity. However, the OsHV-1 μVar virulence may be modulated by biological, physical and chemical factors which are present in sea water. Article in Journal/Newspaper Crassostrea gigas Université des Antilles (UAG): HAL Aquaculture 439 1 6 |
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Open Polar |
collection |
Université des Antilles (UAG): HAL |
op_collection_id |
ftunivantilles |
language |
English |
topic |
Herpesvirus Real-time RT PCR Crassostrea gigas Injection challenge [SDV]Life Sciences [q-bio] |
spellingShingle |
Herpesvirus Real-time RT PCR Crassostrea gigas Injection challenge [SDV]Life Sciences [q-bio] Martenot, Claire Denéchère, Lucie Hubert, Paul Metayer, Laetitia Oden, Elise Trancart, Suzanne Travaillé, Emmanuelle Houssin, Maryline Virulence of Ostreid herpesvirus 1 μVar in sea water at 16 °C and 25 °C |
topic_facet |
Herpesvirus Real-time RT PCR Crassostrea gigas Injection challenge [SDV]Life Sciences [q-bio] |
description |
International audience The Ostreid herpesvirus 1 (OsHV-1) is associated with the mortality of young oysters Crassostrea gigas in France. The virus could infect different marine bivalve species and OsHV-1 DNA was previously detected in crustacean, marine gastropod, and sediment. Nevertheless, the effect of the temperature on the stability of infectious viral particles in sea water remains unpublished. During a mortality event, dead oysters may release virus in the sea water such as free viral particles, viral particles included in cells or attached to a support like plankton, suspended particulate matters, and microalgae. Oysters placed around the infectious source may filtrate the virus and then be infected by OsHV-1. In the present study, we investigated the OsHV-1 μVar virulence in sea water at 16 °C and 25 °C. Filtered infected tissue homogenates were kept at 16 °C or 25 °C prior to injection challenge of uninfected spats. A real-time reverse transcription PCR targeting the OsHV-1 polymerase gene has been developed to confirm the death cause oysters collected during the experiment. The RNA detection of herpesvirus in their hosts is a sign of viral replication and indicates that the virus is certainly infectious. Results showed that the OsHV-1 μVar remained infectious and induced mortality after 33 h at 25 °C and 54 h at 16 °C in controlled conditions. The OsHV-1 μVar is thus able to persist in sea water and high temperatures seem to reduce its infectivity. However, the OsHV-1 μVar virulence may be modulated by biological, physical and chemical factors which are present in sea water. |
author2 |
LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Interactions Cellules Organismes Environnement (ICORE) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) |
format |
Article in Journal/Newspaper |
author |
Martenot, Claire Denéchère, Lucie Hubert, Paul Metayer, Laetitia Oden, Elise Trancart, Suzanne Travaillé, Emmanuelle Houssin, Maryline |
author_facet |
Martenot, Claire Denéchère, Lucie Hubert, Paul Metayer, Laetitia Oden, Elise Trancart, Suzanne Travaillé, Emmanuelle Houssin, Maryline |
author_sort |
Martenot, Claire |
title |
Virulence of Ostreid herpesvirus 1 μVar in sea water at 16 °C and 25 °C |
title_short |
Virulence of Ostreid herpesvirus 1 μVar in sea water at 16 °C and 25 °C |
title_full |
Virulence of Ostreid herpesvirus 1 μVar in sea water at 16 °C and 25 °C |
title_fullStr |
Virulence of Ostreid herpesvirus 1 μVar in sea water at 16 °C and 25 °C |
title_full_unstemmed |
Virulence of Ostreid herpesvirus 1 μVar in sea water at 16 °C and 25 °C |
title_sort |
virulence of ostreid herpesvirus 1 μvar in sea water at 16 °c and 25 °c |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://normandie-univ.hal.science/hal-03402163 https://doi.org/10.1016/j.aquaculture.2015.01.012 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://normandie-univ.hal.science/hal-03402163 Aquaculture, 2015, 439, pp.1-6. ⟨10.1016/j.aquaculture.2015.01.012⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2015.01.012 hal-03402163 https://normandie-univ.hal.science/hal-03402163 doi:10.1016/j.aquaculture.2015.01.012 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2015.01.012 |
container_title |
Aquaculture |
container_volume |
439 |
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1 |
op_container_end_page |
6 |
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1797581629821026304 |