Gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in Crassostrea gigas, a sequential hermaphrodite mollusc
International audience Abstract Background In the animal kingdom, mollusca is an important phylum of the Lophotrochozoa. However, few studies have investigated the molecular cascade of sex determination/early gonadal differentiation within this phylum. The oyster Crassostrea gigas is a sequential ir...
Published in: | BMC Genomics |
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Main Authors: | , , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2021
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Online Access: | https://hal.science/hal-03326966 https://hal.science/hal-03326966/document https://hal.science/hal-03326966/file/Broquard%20et%20al%202021.pdf https://doi.org/10.1186/s12864-021-07838-1 |
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ftunivantilles:oai:HAL:hal-03326966v1 |
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openpolar |
institution |
Open Polar |
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Université des Antilles (UAG): HAL |
op_collection_id |
ftunivantilles |
language |
English |
topic |
Early sex differentiation Gonad transcriptome Irregular hermaphrodite Oyster RNA-Seq Sex determination [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction |
spellingShingle |
Early sex differentiation Gonad transcriptome Irregular hermaphrodite Oyster RNA-Seq Sex determination [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction Broquard, Coralie Saowaros, Suwansa-Ard Lepoittevin, Mélanie Degremont, Lionel Lamy, Jean-Baptiste Morga, Benjamin Elizur, Abigail Martinez, Anne-Sophie Gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in Crassostrea gigas, a sequential hermaphrodite mollusc |
topic_facet |
Early sex differentiation Gonad transcriptome Irregular hermaphrodite Oyster RNA-Seq Sex determination [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction |
description |
International audience Abstract Background In the animal kingdom, mollusca is an important phylum of the Lophotrochozoa. However, few studies have investigated the molecular cascade of sex determination/early gonadal differentiation within this phylum. The oyster Crassostrea gigas is a sequential irregular hermaphrodite mollusc of economic, physiological and phylogenetic importance. Although some studies identified genes of its sex-determining/−differentiating pathway, this particular topic remains to be further deepened, in particular with regard to the expression patterns. Indeed, these patterns need to cover the entire period of sex lability and have to be associated to future sex phenotypes, usually impossible to establish in this sequential hermaphrodite. This is why we performed a gonadal RNA-Seq analysis of diploid male and female oysters that have not changed sex for 4 years, sampled during the entire time-window of sex determination/early sex differentiation (stages 0 and 3 of the gametogenetic cycle). This individual long-term monitoring gave us the opportunity to explain the molecular expression patterns in the light of the most statistically likely future sex of each oyster. Results The differential gene expression analysis of gonadal transcriptomes revealed that 9723 genes were differentially expressed between gametogenetic stages, and 141 between sexes (98 and 43 genes highly expressed in females and males, respectively). Eighty-four genes were both stage- and sex-specific, 57 of them being highly expressed at the time of sex determination/early sex differentiation. These 4 novel genes including Trophoblast glycoprotein-like, Protein PML-like, Protein singed-like and PREDICTED: paramyosin, while being supported by RT-qPCR, displayed sexually dimorphic gene expression patterns. Conclusions This gonadal transcriptome analysis, the first one associated with sex phenotypes in C. gigas , revealed 57 genes highly expressed in stage 0 or 3 of gametogenesis and which could be linked to the future sex of ... |
author2 |
Université de Caen Normandie (UNICAEN) Normandie Université (NU) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Mahidol University Bangkok University of the Sunshine Coast (USC) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
format |
Article in Journal/Newspaper |
author |
Broquard, Coralie Saowaros, Suwansa-Ard Lepoittevin, Mélanie Degremont, Lionel Lamy, Jean-Baptiste Morga, Benjamin Elizur, Abigail Martinez, Anne-Sophie |
author_facet |
Broquard, Coralie Saowaros, Suwansa-Ard Lepoittevin, Mélanie Degremont, Lionel Lamy, Jean-Baptiste Morga, Benjamin Elizur, Abigail Martinez, Anne-Sophie |
author_sort |
Broquard, Coralie |
title |
Gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in Crassostrea gigas, a sequential hermaphrodite mollusc |
title_short |
Gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in Crassostrea gigas, a sequential hermaphrodite mollusc |
title_full |
Gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in Crassostrea gigas, a sequential hermaphrodite mollusc |
title_fullStr |
Gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in Crassostrea gigas, a sequential hermaphrodite mollusc |
title_full_unstemmed |
Gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in Crassostrea gigas, a sequential hermaphrodite mollusc |
title_sort |
gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in crassostrea gigas, a sequential hermaphrodite mollusc |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.science/hal-03326966 https://hal.science/hal-03326966/document https://hal.science/hal-03326966/file/Broquard%20et%20al%202021.pdf https://doi.org/10.1186/s12864-021-07838-1 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1471-2164 BMC Genomics https://hal.science/hal-03326966 BMC Genomics, 2021, 22 (1), ⟨10.1186/s12864-021-07838-1⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-021-07838-1 info:eu-repo/semantics/altIdentifier/pmid/34372770 hal-03326966 https://hal.science/hal-03326966 https://hal.science/hal-03326966/document https://hal.science/hal-03326966/file/Broquard%20et%20al%202021.pdf doi:10.1186/s12864-021-07838-1 PUBMED: 34372770 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/s12864-021-07838-1 |
container_title |
BMC Genomics |
container_volume |
22 |
container_issue |
1 |
_version_ |
1797581660311519232 |
spelling |
ftunivantilles:oai:HAL:hal-03326966v1 2024-04-28T08:16:36+00:00 Gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in Crassostrea gigas, a sequential hermaphrodite mollusc Broquard, Coralie Saowaros, Suwansa-Ard Lepoittevin, Mélanie Degremont, Lionel Lamy, Jean-Baptiste Morga, Benjamin Elizur, Abigail Martinez, Anne-Sophie Université de Caen Normandie (UNICAEN) Normandie Université (NU) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Mahidol University Bangkok University of the Sunshine Coast (USC) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) 2021-12 https://hal.science/hal-03326966 https://hal.science/hal-03326966/document https://hal.science/hal-03326966/file/Broquard%20et%20al%202021.pdf https://doi.org/10.1186/s12864-021-07838-1 en eng HAL CCSD BioMed Central info:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-021-07838-1 info:eu-repo/semantics/altIdentifier/pmid/34372770 hal-03326966 https://hal.science/hal-03326966 https://hal.science/hal-03326966/document https://hal.science/hal-03326966/file/Broquard%20et%20al%202021.pdf doi:10.1186/s12864-021-07838-1 PUBMED: 34372770 info:eu-repo/semantics/OpenAccess ISSN: 1471-2164 BMC Genomics https://hal.science/hal-03326966 BMC Genomics, 2021, 22 (1), ⟨10.1186/s12864-021-07838-1⟩ Early sex differentiation Gonad transcriptome Irregular hermaphrodite Oyster RNA-Seq Sex determination [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction info:eu-repo/semantics/article Journal articles 2021 ftunivantilles https://doi.org/10.1186/s12864-021-07838-1 2024-04-17T23:47:11Z International audience Abstract Background In the animal kingdom, mollusca is an important phylum of the Lophotrochozoa. However, few studies have investigated the molecular cascade of sex determination/early gonadal differentiation within this phylum. The oyster Crassostrea gigas is a sequential irregular hermaphrodite mollusc of economic, physiological and phylogenetic importance. Although some studies identified genes of its sex-determining/−differentiating pathway, this particular topic remains to be further deepened, in particular with regard to the expression patterns. Indeed, these patterns need to cover the entire period of sex lability and have to be associated to future sex phenotypes, usually impossible to establish in this sequential hermaphrodite. This is why we performed a gonadal RNA-Seq analysis of diploid male and female oysters that have not changed sex for 4 years, sampled during the entire time-window of sex determination/early sex differentiation (stages 0 and 3 of the gametogenetic cycle). This individual long-term monitoring gave us the opportunity to explain the molecular expression patterns in the light of the most statistically likely future sex of each oyster. Results The differential gene expression analysis of gonadal transcriptomes revealed that 9723 genes were differentially expressed between gametogenetic stages, and 141 between sexes (98 and 43 genes highly expressed in females and males, respectively). Eighty-four genes were both stage- and sex-specific, 57 of them being highly expressed at the time of sex determination/early sex differentiation. These 4 novel genes including Trophoblast glycoprotein-like, Protein PML-like, Protein singed-like and PREDICTED: paramyosin, while being supported by RT-qPCR, displayed sexually dimorphic gene expression patterns. Conclusions This gonadal transcriptome analysis, the first one associated with sex phenotypes in C. gigas , revealed 57 genes highly expressed in stage 0 or 3 of gametogenesis and which could be linked to the future sex of ... Article in Journal/Newspaper Crassostrea gigas Université des Antilles (UAG): HAL BMC Genomics 22 1 |