Redefinition of Aureobasidium pullulans and its varieties
Using media with low water activity, a large numbers of aureobasidium-like black yeasts were isolated from glacial and subglacial ice of three polythermal glaciers from the coastal Arctic environment of Kongsfjorden (Svalbard, Spitsbergen), as well as from adjacent sea water, sea ice and glacial mel...
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ftunivamstpubl:oai:uvapub:308520 2023-05-15T14:51:37+02:00 Redefinition of Aureobasidium pullulans and its varieties P. Zalar C. Gostinčar G.S. de Hoog V. Uršič M. Sudhadham N. Gunde-Cimerman 2008 http://hdl.handle.net/11245/1.308520 unknown 10.3114/sim.2008.61.02 It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content licence (like Creative Commons). Studies in Mycology (01660616) vol.61 (2008) nr.1 p.21-38 article 2008 ftunivamstpubl 2016-08-10T22:13:22Z Using media with low water activity, a large numbers of aureobasidium-like black yeasts were isolated from glacial and subglacial ice of three polythermal glaciers from the coastal Arctic environment of Kongsfjorden (Svalbard, Spitsbergen), as well as from adjacent sea water, sea ice and glacial meltwaters. To characterise the genetic variability of Aureobasidium pullulans strains originating from the Arctic and strains originating pan-globally, a multilocus molecular analysis was performed, through rDNA (internal transcribed spacers, partial 28 S rDNA), and partial introns and exons of genes encoding β-tubulin (TUB), translation elongation factor (EF1{alpha}) and elongase (ELO). Two globally ubiquitous varieties were distinguished: var. pullulans, occurring particularly in slightly osmotic substrates and in the phyllosphere; and var. melanogenum, mainly isolated from watery habitats. Both varieties were commonly isolated from the sampled Arctic habitats. However, some aureobasidium-like strains from subglacial ice from three different glaciers in Kongsfjorden (Svalbard, Spitsbergen), appeared to represent a new variety of A. pullulans. A strain from dolomitic marble in Namibia was found to belong to yet another variety. No molecular support has as yet been found for the previously described var. aubasidani. A partial elongase-encoding gene was successfully used as a phylogenetic marker at the (infra-)specific level. Article in Journal/Newspaper Arctic Kongsfjord* Kongsfjorden Sea ice Svalbard Spitsbergen Universiteit van Amsterdam: Digital Academic Repository (UvA DARE) Arctic Svalbard |
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Open Polar |
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Universiteit van Amsterdam: Digital Academic Repository (UvA DARE) |
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ftunivamstpubl |
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unknown |
description |
Using media with low water activity, a large numbers of aureobasidium-like black yeasts were isolated from glacial and subglacial ice of three polythermal glaciers from the coastal Arctic environment of Kongsfjorden (Svalbard, Spitsbergen), as well as from adjacent sea water, sea ice and glacial meltwaters. To characterise the genetic variability of Aureobasidium pullulans strains originating from the Arctic and strains originating pan-globally, a multilocus molecular analysis was performed, through rDNA (internal transcribed spacers, partial 28 S rDNA), and partial introns and exons of genes encoding β-tubulin (TUB), translation elongation factor (EF1{alpha}) and elongase (ELO). Two globally ubiquitous varieties were distinguished: var. pullulans, occurring particularly in slightly osmotic substrates and in the phyllosphere; and var. melanogenum, mainly isolated from watery habitats. Both varieties were commonly isolated from the sampled Arctic habitats. However, some aureobasidium-like strains from subglacial ice from three different glaciers in Kongsfjorden (Svalbard, Spitsbergen), appeared to represent a new variety of A. pullulans. A strain from dolomitic marble in Namibia was found to belong to yet another variety. No molecular support has as yet been found for the previously described var. aubasidani. A partial elongase-encoding gene was successfully used as a phylogenetic marker at the (infra-)specific level. |
format |
Article in Journal/Newspaper |
author |
P. Zalar C. Gostinčar G.S. de Hoog V. Uršič M. Sudhadham N. Gunde-Cimerman |
spellingShingle |
P. Zalar C. Gostinčar G.S. de Hoog V. Uršič M. Sudhadham N. Gunde-Cimerman Redefinition of Aureobasidium pullulans and its varieties |
author_facet |
P. Zalar C. Gostinčar G.S. de Hoog V. Uršič M. Sudhadham N. Gunde-Cimerman |
author_sort |
P. Zalar |
title |
Redefinition of Aureobasidium pullulans and its varieties |
title_short |
Redefinition of Aureobasidium pullulans and its varieties |
title_full |
Redefinition of Aureobasidium pullulans and its varieties |
title_fullStr |
Redefinition of Aureobasidium pullulans and its varieties |
title_full_unstemmed |
Redefinition of Aureobasidium pullulans and its varieties |
title_sort |
redefinition of aureobasidium pullulans and its varieties |
publishDate |
2008 |
url |
http://hdl.handle.net/11245/1.308520 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Kongsfjord* Kongsfjorden Sea ice Svalbard Spitsbergen |
genre_facet |
Arctic Kongsfjord* Kongsfjorden Sea ice Svalbard Spitsbergen |
op_source |
Studies in Mycology (01660616) vol.61 (2008) nr.1 p.21-38 |
op_relation |
10.3114/sim.2008.61.02 |
op_rights |
It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content licence (like Creative Commons). |
_version_ |
1766322755382280192 |