Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges
Sponges produce distinct fatty acids (FAs) that (potentially) can be used as chemotaxonomic and ecological biomarkers to study endosymbiont-host interactions and the functional ecology of sponges. Here, we present FA profiles of five common habitat-building deep-sea sponges (class Demospongiae, orde...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2021
|
Subjects: | |
Online Access: | https://dare.uva.nl/personal/pure/en/publications/bacterial-precursors-and-unsaturated-longchain-fatty-acids-are-biomarkers-of-northatlantic-deepsea-demosponges(55ee1f9b-a3e6-4f0c-a12c-77525ce40df0).html https://doi.org/10.1371/journal.pone.0241095 https://hdl.handle.net/11245.1/55ee1f9b-a3e6-4f0c-a12c-77525ce40df0 https://pure.uva.nl/ws/files/68942315/journal.pone.0241095_2_.pdf http://www.scopus.com/inward/record.url?scp=85100387946&partnerID=8YFLogxK |
id |
ftunivamstpubl:oai:dare.uva.nl:openaire_cris_publications/55ee1f9b-a3e6-4f0c-a12c-77525ce40df0 |
---|---|
record_format |
openpolar |
spelling |
ftunivamstpubl:oai:dare.uva.nl:openaire_cris_publications/55ee1f9b-a3e6-4f0c-a12c-77525ce40df0 2024-09-30T14:31:37+00:00 Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges de Kluijver, A. Nierop, K.G.J. Morganti, T.M. Bart, M.C. Slaby, B.M. Hanz, U. de Goeij, J.M. Mienis, F. Middelburg, J.J. 2021-01-27 application/pdf https://dare.uva.nl/personal/pure/en/publications/bacterial-precursors-and-unsaturated-longchain-fatty-acids-are-biomarkers-of-northatlantic-deepsea-demosponges(55ee1f9b-a3e6-4f0c-a12c-77525ce40df0).html https://doi.org/10.1371/journal.pone.0241095 https://hdl.handle.net/11245.1/55ee1f9b-a3e6-4f0c-a12c-77525ce40df0 https://pure.uva.nl/ws/files/68942315/journal.pone.0241095_2_.pdf http://www.scopus.com/inward/record.url?scp=85100387946&partnerID=8YFLogxK eng eng https://dare.uva.nl/personal/pure/en/publications/bacterial-precursors-and-unsaturated-longchain-fatty-acids-are-biomarkers-of-northatlantic-deepsea-demosponges(55ee1f9b-a3e6-4f0c-a12c-77525ce40df0).html info:eu-repo/semantics/openAccess de Kluijver , A , Nierop , K G J , Morganti , T M , Bart , M C , Slaby , B M , Hanz , U , de Goeij , J M , Mienis , F & Middelburg , J J 2021 , ' Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges ' , PLoS ONE , vol. 16 , no. 1 , e0241095 . https://doi.org/10.1371/journal.pone.0241095 article 2021 ftunivamstpubl https://doi.org/10.1371/journal.pone.0241095 2024-09-12T16:38:40Z Sponges produce distinct fatty acids (FAs) that (potentially) can be used as chemotaxonomic and ecological biomarkers to study endosymbiont-host interactions and the functional ecology of sponges. Here, we present FA profiles of five common habitat-building deep-sea sponges (class Demospongiae, order Tetractinellida), which are classified as high microbial abundance (HMA) species. Geodia hentscheli , G. parva, G. atlantica, G. barretti, and Stelletta rhaphidiophora were collected from boreal and Arctic sponge grounds in the North-Atlantic Ocean. Bacterial FAs dominated in all five species and particularly isomeric mixtures of mid-chain branched FAs (MBFAs, 8- and 9-Me-C 16:0 and 10- and 11-Me-C 18:0 ) were found in high abundance (together ≥ 20% of total FAs) aside more common bacterial markers. In addition, the sponges produced long-chain linear, mid- and a(i)-branched unsaturated FAs (LCFAs) with a chain length of 24–28 C atoms and had predominantly the typical Δ 5,9 unsaturation, although the Δ 9,19 and (yet undescribed) Δ 11,21 unsaturations were also identified. G. parva and S. rhaphidiophora each produced distinct LCFAs, while G. atlantica, G. barretti, and G. hentscheli produced similar LCFAs, but in different ratios. The different bacterial precursors varied in carbon isotopic composition (δ 13 C), with MBFAs being more enriched compared to other bacterial (linear and a(i)-branched) FAs. We propose biosynthetic pathways for different LCFAs from their bacterial precursors, that are consistent with small isotopic differences found in LCFAs. Indeed, FA profiles of deep-sea sponges can serve as chemotaxonomic markers and support the concept that sponges acquire building blocks from their endosymbiotic bacteria. Article in Journal/Newspaper Arctic North Atlantic Universiteit van Amsterdam: Digital Academic Repository (UvA DARE) Arctic PLOS ONE 16 1 e0241095 |
institution |
Open Polar |
collection |
Universiteit van Amsterdam: Digital Academic Repository (UvA DARE) |
op_collection_id |
ftunivamstpubl |
language |
English |
description |
Sponges produce distinct fatty acids (FAs) that (potentially) can be used as chemotaxonomic and ecological biomarkers to study endosymbiont-host interactions and the functional ecology of sponges. Here, we present FA profiles of five common habitat-building deep-sea sponges (class Demospongiae, order Tetractinellida), which are classified as high microbial abundance (HMA) species. Geodia hentscheli , G. parva, G. atlantica, G. barretti, and Stelletta rhaphidiophora were collected from boreal and Arctic sponge grounds in the North-Atlantic Ocean. Bacterial FAs dominated in all five species and particularly isomeric mixtures of mid-chain branched FAs (MBFAs, 8- and 9-Me-C 16:0 and 10- and 11-Me-C 18:0 ) were found in high abundance (together ≥ 20% of total FAs) aside more common bacterial markers. In addition, the sponges produced long-chain linear, mid- and a(i)-branched unsaturated FAs (LCFAs) with a chain length of 24–28 C atoms and had predominantly the typical Δ 5,9 unsaturation, although the Δ 9,19 and (yet undescribed) Δ 11,21 unsaturations were also identified. G. parva and S. rhaphidiophora each produced distinct LCFAs, while G. atlantica, G. barretti, and G. hentscheli produced similar LCFAs, but in different ratios. The different bacterial precursors varied in carbon isotopic composition (δ 13 C), with MBFAs being more enriched compared to other bacterial (linear and a(i)-branched) FAs. We propose biosynthetic pathways for different LCFAs from their bacterial precursors, that are consistent with small isotopic differences found in LCFAs. Indeed, FA profiles of deep-sea sponges can serve as chemotaxonomic markers and support the concept that sponges acquire building blocks from their endosymbiotic bacteria. |
format |
Article in Journal/Newspaper |
author |
de Kluijver, A. Nierop, K.G.J. Morganti, T.M. Bart, M.C. Slaby, B.M. Hanz, U. de Goeij, J.M. Mienis, F. Middelburg, J.J. |
spellingShingle |
de Kluijver, A. Nierop, K.G.J. Morganti, T.M. Bart, M.C. Slaby, B.M. Hanz, U. de Goeij, J.M. Mienis, F. Middelburg, J.J. Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges |
author_facet |
de Kluijver, A. Nierop, K.G.J. Morganti, T.M. Bart, M.C. Slaby, B.M. Hanz, U. de Goeij, J.M. Mienis, F. Middelburg, J.J. |
author_sort |
de Kluijver, A. |
title |
Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges |
title_short |
Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges |
title_full |
Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges |
title_fullStr |
Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges |
title_full_unstemmed |
Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges |
title_sort |
bacterial precursors and unsaturated long-chain fatty acids are biomarkers of north-atlantic deep-sea demosponges |
publishDate |
2021 |
url |
https://dare.uva.nl/personal/pure/en/publications/bacterial-precursors-and-unsaturated-longchain-fatty-acids-are-biomarkers-of-northatlantic-deepsea-demosponges(55ee1f9b-a3e6-4f0c-a12c-77525ce40df0).html https://doi.org/10.1371/journal.pone.0241095 https://hdl.handle.net/11245.1/55ee1f9b-a3e6-4f0c-a12c-77525ce40df0 https://pure.uva.nl/ws/files/68942315/journal.pone.0241095_2_.pdf http://www.scopus.com/inward/record.url?scp=85100387946&partnerID=8YFLogxK |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic North Atlantic |
genre_facet |
Arctic North Atlantic |
op_source |
de Kluijver , A , Nierop , K G J , Morganti , T M , Bart , M C , Slaby , B M , Hanz , U , de Goeij , J M , Mienis , F & Middelburg , J J 2021 , ' Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges ' , PLoS ONE , vol. 16 , no. 1 , e0241095 . https://doi.org/10.1371/journal.pone.0241095 |
op_relation |
https://dare.uva.nl/personal/pure/en/publications/bacterial-precursors-and-unsaturated-longchain-fatty-acids-are-biomarkers-of-northatlantic-deepsea-demosponges(55ee1f9b-a3e6-4f0c-a12c-77525ce40df0).html |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0241095 |
container_title |
PLOS ONE |
container_volume |
16 |
container_issue |
1 |
container_start_page |
e0241095 |
_version_ |
1811636073956114432 |