Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis
This paper studies the synthesis of triacylglycerols (TAGs) rich in palmitic acid (PA) at sn-2 position from palm stearin (PS), a vegetable oil highly rich in PA (60%, but only 12.8% of this is located at sn-2 position). These PA rich TAGs were obtained by lipase-catalyzed acidolysis of this oil wit...
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Biochemical Engineering Journal
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ftunivalmeria:oai:repositorio.ual.es:10835/63 2023-10-09T21:46:26+02:00 Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis Jiménez Callejón, María José Esteban Cerdán, Luis Robles Medina, Alfonso Hita Peña, Estrella González Moreno, Pedro Antonio Muñío Martínez, María del Mar Molina Grima, Emilio 2010 http://hdl.handle.net/10835/63 en eng Biochemical Engineering Journal Biochemical Engineering Journal, 51 (2010) 172-179 http://hdl.handle.net/10835/63 info:eu-repo/semantics/openAccess palm sterin palmitic acid triacylglycerols acidolysis lipase QLC purification of triacylglycerols info:eu-repo/semantics/article 2010 ftunivalmeria 2023-09-19T15:18:14Z This paper studies the synthesis of triacylglycerols (TAGs) rich in palmitic acid (PA) at sn-2 position from palm stearin (PS), a vegetable oil highly rich in PA (60%, but only 12.8% of this is located at sn-2 position). These PA rich TAGs were obtained by lipase-catalyzed acidolysis of this oil with free fatty acids (FFAs) highly rich in PA, such as commercial PA (98% PA) and a FFA extract obtained by saponification of PS (60% PA). PA has a melting point of 63 ºC and during the acidolysis reaction the substrates, highly rich in this acid, must remain liquid; therefore high temperatures or solvents must be used. An important objective of this work was to operate without solvent and at the lowest possible temperature. In this acidolysis reaction four factors were firstly studied: type of lipase, temperature, solvent amount and the intensity of treatment (IOT = lipase amount × reaction time/PS amount). The influence of these variables was studied in a stirred tank reactor (STR). The lipases tested were Novozym 435 from Candida antarctica (immobilized on a macroporous acrylic resin), and lipases QLC (immobilized on diatomaceous earth), and QLM (non immobilized), both from Alcaligenes sp., and the one selected was lipase QLC. According to the manufacturer the optimum temperature for this lipase is 65-70 ºC, which allows it to operate without solvent. The best results with lipase QLC (TAGs with 80% PA, both total and at sn-2 position) were obtained with commercial PA, at 65 ºC, a 3:1 FFA/PS molar ratio (1:1 w/w), without solvent and an IOT = 7 g lipase × h/g PS (for example 2.5 g PS, 2.5 g commercial PA, 0.75 g lipase and 24 h). These results were the basis for establishing the operational conditions to obtain PA rich TAGs with the lipase immobilized in a packed bed reactor (PBR), operating by recirculation of the reaction mixture through the lipase bed. In this system TAGs with 75% PA were obtained at an IOT = 8 g lipase × h/g PS. This result and the apparent kinetic constants obtained in both reactors show that the ... Article in Journal/Newspaper Antarc* Antarctica Universidad de Almería: Repositorio Institucional |
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Open Polar |
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Universidad de Almería: Repositorio Institucional |
op_collection_id |
ftunivalmeria |
language |
English |
topic |
palm sterin palmitic acid triacylglycerols acidolysis lipase QLC purification of triacylglycerols |
spellingShingle |
palm sterin palmitic acid triacylglycerols acidolysis lipase QLC purification of triacylglycerols Jiménez Callejón, María José Esteban Cerdán, Luis Robles Medina, Alfonso Hita Peña, Estrella González Moreno, Pedro Antonio Muñío Martínez, María del Mar Molina Grima, Emilio Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis |
topic_facet |
palm sterin palmitic acid triacylglycerols acidolysis lipase QLC purification of triacylglycerols |
description |
This paper studies the synthesis of triacylglycerols (TAGs) rich in palmitic acid (PA) at sn-2 position from palm stearin (PS), a vegetable oil highly rich in PA (60%, but only 12.8% of this is located at sn-2 position). These PA rich TAGs were obtained by lipase-catalyzed acidolysis of this oil with free fatty acids (FFAs) highly rich in PA, such as commercial PA (98% PA) and a FFA extract obtained by saponification of PS (60% PA). PA has a melting point of 63 ºC and during the acidolysis reaction the substrates, highly rich in this acid, must remain liquid; therefore high temperatures or solvents must be used. An important objective of this work was to operate without solvent and at the lowest possible temperature. In this acidolysis reaction four factors were firstly studied: type of lipase, temperature, solvent amount and the intensity of treatment (IOT = lipase amount × reaction time/PS amount). The influence of these variables was studied in a stirred tank reactor (STR). The lipases tested were Novozym 435 from Candida antarctica (immobilized on a macroporous acrylic resin), and lipases QLC (immobilized on diatomaceous earth), and QLM (non immobilized), both from Alcaligenes sp., and the one selected was lipase QLC. According to the manufacturer the optimum temperature for this lipase is 65-70 ºC, which allows it to operate without solvent. The best results with lipase QLC (TAGs with 80% PA, both total and at sn-2 position) were obtained with commercial PA, at 65 ºC, a 3:1 FFA/PS molar ratio (1:1 w/w), without solvent and an IOT = 7 g lipase × h/g PS (for example 2.5 g PS, 2.5 g commercial PA, 0.75 g lipase and 24 h). These results were the basis for establishing the operational conditions to obtain PA rich TAGs with the lipase immobilized in a packed bed reactor (PBR), operating by recirculation of the reaction mixture through the lipase bed. In this system TAGs with 75% PA were obtained at an IOT = 8 g lipase × h/g PS. This result and the apparent kinetic constants obtained in both reactors show that the ... |
format |
Article in Journal/Newspaper |
author |
Jiménez Callejón, María José Esteban Cerdán, Luis Robles Medina, Alfonso Hita Peña, Estrella González Moreno, Pedro Antonio Muñío Martínez, María del Mar Molina Grima, Emilio |
author_facet |
Jiménez Callejón, María José Esteban Cerdán, Luis Robles Medina, Alfonso Hita Peña, Estrella González Moreno, Pedro Antonio Muñío Martínez, María del Mar Molina Grima, Emilio |
author_sort |
Jiménez Callejón, María José |
title |
Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis |
title_short |
Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis |
title_full |
Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis |
title_fullStr |
Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis |
title_full_unstemmed |
Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis |
title_sort |
production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis |
publisher |
Biochemical Engineering Journal |
publishDate |
2010 |
url |
http://hdl.handle.net/10835/63 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Biochemical Engineering Journal, 51 (2010) 172-179 http://hdl.handle.net/10835/63 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1779322127359410176 |