Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification
The aim of this work was to obtain biodiesel (methyl esters) from the saponifiable lipids (SLs) fraction of the microalga Nannochloropsis gaditana, whose biomass dry weight contains 12.1 wt% of these lipids. SLs were extracted from the microalga as free fatty acids (FFAs) for subsequent transformati...
Published in: | Journal of Bioscience and Bioengineering |
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2015
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Online Access: | http://hdl.handle.net/10835/15043 |
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ftunivalmeria:oai:repositorio.ual.es:10835/15043 2024-02-11T09:56:13+01:00 Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification Castillo López, Beatriz Esteban Cerdán, Luis Robles Medina, Alfonso Navarro López, Elvira Martín Valverde, Lorena Hita Peña, Estrella González Moreno, Pedro Antonio Molina Grima, Emilio 2015-06-01 http://hdl.handle.net/10835/15043 en eng https://doi.org/10.1016/j.jbiosc.2014.11.002 13891723 http://hdl.handle.net/10835/15043 info:eu-repo/semantics/openAccess biodiesel microalga Nannochloropsis gaditana lipase Novozym 435 free fatty acid used vegetable oil info:eu-repo/semantics/article 2015 ftunivalmeria https://doi.org/10.1016/j.jbiosc.2014.11.002 2024-01-17T00:19:03Z The aim of this work was to obtain biodiesel (methyl esters) from the saponifiable lipids (SLs) fraction of the microalga Nannochloropsis gaditana, whose biomass dry weight contains 12.1 wt% of these lipids. SLs were extracted from the microalga as free fatty acids (FFAs) for subsequent transformation to methyl esters (biodiesel) by enzymatic esterification. Extraction as FFAs rather than as SLs allows them to be obtained with higher purity. Microalgal FFAs were obtained by direct saponification of lipids in the biomass and subsequent extraction-purification with hexane. Esterification of FFAs with methanol was catalysed by lipase Novozym 435 from Candida antarctica. Stability studies of this lipase in the operational conditions showed that the esterification degree (ED) attained with the same batch of lipase remained constant over six reaction cycles (36 h total reaction time). The optimal conditions attained for 4 g of FFAs were 25 ºC, 200 rpm, methanol/FFA molar ratio of 1.5:1, Novozym 435/FFA ratio of 0.025:1 w/w and 4 h reaction time. In these conditions the ED attained was 92.6%, producing a biodiesel with 83 wt% purity from microalgal FFAs. Several experimental scales were tested (from 4 to 40 g FFAs), and in all cases similar EDs were obtained. Article in Journal/Newspaper Antarc* Antarctica Universidad de Almería: Repositorio Institucional Journal of Bioscience and Bioengineering 119 6 706 711 |
institution |
Open Polar |
collection |
Universidad de Almería: Repositorio Institucional |
op_collection_id |
ftunivalmeria |
language |
English |
topic |
biodiesel microalga Nannochloropsis gaditana lipase Novozym 435 free fatty acid used vegetable oil |
spellingShingle |
biodiesel microalga Nannochloropsis gaditana lipase Novozym 435 free fatty acid used vegetable oil Castillo López, Beatriz Esteban Cerdán, Luis Robles Medina, Alfonso Navarro López, Elvira Martín Valverde, Lorena Hita Peña, Estrella González Moreno, Pedro Antonio Molina Grima, Emilio Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
topic_facet |
biodiesel microalga Nannochloropsis gaditana lipase Novozym 435 free fatty acid used vegetable oil |
description |
The aim of this work was to obtain biodiesel (methyl esters) from the saponifiable lipids (SLs) fraction of the microalga Nannochloropsis gaditana, whose biomass dry weight contains 12.1 wt% of these lipids. SLs were extracted from the microalga as free fatty acids (FFAs) for subsequent transformation to methyl esters (biodiesel) by enzymatic esterification. Extraction as FFAs rather than as SLs allows them to be obtained with higher purity. Microalgal FFAs were obtained by direct saponification of lipids in the biomass and subsequent extraction-purification with hexane. Esterification of FFAs with methanol was catalysed by lipase Novozym 435 from Candida antarctica. Stability studies of this lipase in the operational conditions showed that the esterification degree (ED) attained with the same batch of lipase remained constant over six reaction cycles (36 h total reaction time). The optimal conditions attained for 4 g of FFAs were 25 ºC, 200 rpm, methanol/FFA molar ratio of 1.5:1, Novozym 435/FFA ratio of 0.025:1 w/w and 4 h reaction time. In these conditions the ED attained was 92.6%, producing a biodiesel with 83 wt% purity from microalgal FFAs. Several experimental scales were tested (from 4 to 40 g FFAs), and in all cases similar EDs were obtained. |
format |
Article in Journal/Newspaper |
author |
Castillo López, Beatriz Esteban Cerdán, Luis Robles Medina, Alfonso Navarro López, Elvira Martín Valverde, Lorena Hita Peña, Estrella González Moreno, Pedro Antonio Molina Grima, Emilio |
author_facet |
Castillo López, Beatriz Esteban Cerdán, Luis Robles Medina, Alfonso Navarro López, Elvira Martín Valverde, Lorena Hita Peña, Estrella González Moreno, Pedro Antonio Molina Grima, Emilio |
author_sort |
Castillo López, Beatriz |
title |
Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
title_short |
Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
title_full |
Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
title_fullStr |
Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
title_full_unstemmed |
Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
title_sort |
production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
publishDate |
2015 |
url |
http://hdl.handle.net/10835/15043 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://doi.org/10.1016/j.jbiosc.2014.11.002 13891723 http://hdl.handle.net/10835/15043 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.jbiosc.2014.11.002 |
container_title |
Journal of Bioscience and Bioengineering |
container_volume |
119 |
container_issue |
6 |
container_start_page |
706 |
op_container_end_page |
711 |
_version_ |
1790601415834468352 |