Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula
In Antarctic coastal waters where nutrient limitations are low, viruses are expected to play a major role in the regulation of bloom events. Despite this, research in viral identification and dynamics is scarce, with limited information available for the Southern Ocean (SO). This study presents an i...
Published in: | Frontiers in Microbiology |
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Online Access: | http://hdl.handle.net/10045/91765 https://doi.org/10.3389/fmicb.2019.01014 |
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ftunivalicante:oai:rua.ua.es:10045/91765 2023-05-15T14:04:56+02:00 Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula Alarcón-Schumacher, Tomás Guajardo-Leiva, Sergio Anton, Josefa Díez, Beatriz Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología Ecología Microbiana Molecular 2019-05-14 http://hdl.handle.net/10045/91765 https://doi.org/10.3389/fmicb.2019.01014 eng eng Frontiers Media https://doi.org/10.3389/fmicb.2019.01014 Alarcón-Schumacher T, Guajardo-Leiva S, Antón J and Díez B (2019) Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula. Front. Microbiol. 10:1014. doi:10.3389/fmicb.2019.01014 1664-302X http://hdl.handle.net/10045/91765 doi:10.3389/fmicb.2019.01014 © 2019 Alarcón-Schumacher, Guajardo-Leiva, Antón and Díez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. info:eu-repo/semantics/openAccess CC-BY Phytoplankton blooms Southern Ocean Viruses Environmental genomics Viral ecology Microbiología info:eu-repo/semantics/article 2019 ftunivalicante https://doi.org/10.3389/fmicb.2019.01014 2020-06-05T13:20:45Z In Antarctic coastal waters where nutrient limitations are low, viruses are expected to play a major role in the regulation of bloom events. Despite this, research in viral identification and dynamics is scarce, with limited information available for the Southern Ocean (SO). This study presents an integrative-omics approach, comparing variation in the viral and microbial active communities on two contrasting sample conditions from a diatom-dominated phytoplankton bloom occurring in Chile Bay in the West Antarctic Peninsula (WAP) in the summer of 2014. The known viral community, initially dominated by Myoviridae family (∼82% of the total assigned reads), changed to become dominated by Phycodnaviridae (∼90%), while viral activity was predominantly driven by dsDNA members of the Phycodnaviridae (∼50%) and diatom infecting ssRNA viruses (∼38%), becoming more significant as chlorophyll a increased. A genomic and phylogenetic characterization allowed the identification of a new viral lineage within the Myoviridae family. This new lineage of viruses infects Pseudoalteromonas and was dominant in the phage community. In addition, a new Phycodnavirus (PaV) was described, which is predicted to infect Phaeocystis antarctica, the main blooming haptophyte in the SO. This work was able to identify the changes in the main viral players during a bloom development and suggests that the changes observed in the virioplankton could be used as a model to understand the development and decay of blooms that occur throughout the WAP. This work was financially supported by a Ph.D. scholarship CONICYT N° 21130667; INACH15-10, INACH RG_09-17, CONICYT for international cooperation DPI20140044, FONDAP N° 15110009, and FONDECYT N° 1190998. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Southern Ocean RUA - Repositorio Institucional de la Universidad de Alicante Antarctic Antarctic Peninsula Inach ENVELOPE(-60.783,-60.783,-62.467,-62.467) Southern Ocean Frontiers in Microbiology 10 |
institution |
Open Polar |
collection |
RUA - Repositorio Institucional de la Universidad de Alicante |
op_collection_id |
ftunivalicante |
language |
English |
topic |
Phytoplankton blooms Southern Ocean Viruses Environmental genomics Viral ecology Microbiología |
spellingShingle |
Phytoplankton blooms Southern Ocean Viruses Environmental genomics Viral ecology Microbiología Alarcón-Schumacher, Tomás Guajardo-Leiva, Sergio Anton, Josefa Díez, Beatriz Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula |
topic_facet |
Phytoplankton blooms Southern Ocean Viruses Environmental genomics Viral ecology Microbiología |
description |
In Antarctic coastal waters where nutrient limitations are low, viruses are expected to play a major role in the regulation of bloom events. Despite this, research in viral identification and dynamics is scarce, with limited information available for the Southern Ocean (SO). This study presents an integrative-omics approach, comparing variation in the viral and microbial active communities on two contrasting sample conditions from a diatom-dominated phytoplankton bloom occurring in Chile Bay in the West Antarctic Peninsula (WAP) in the summer of 2014. The known viral community, initially dominated by Myoviridae family (∼82% of the total assigned reads), changed to become dominated by Phycodnaviridae (∼90%), while viral activity was predominantly driven by dsDNA members of the Phycodnaviridae (∼50%) and diatom infecting ssRNA viruses (∼38%), becoming more significant as chlorophyll a increased. A genomic and phylogenetic characterization allowed the identification of a new viral lineage within the Myoviridae family. This new lineage of viruses infects Pseudoalteromonas and was dominant in the phage community. In addition, a new Phycodnavirus (PaV) was described, which is predicted to infect Phaeocystis antarctica, the main blooming haptophyte in the SO. This work was able to identify the changes in the main viral players during a bloom development and suggests that the changes observed in the virioplankton could be used as a model to understand the development and decay of blooms that occur throughout the WAP. This work was financially supported by a Ph.D. scholarship CONICYT N° 21130667; INACH15-10, INACH RG_09-17, CONICYT for international cooperation DPI20140044, FONDAP N° 15110009, and FONDECYT N° 1190998. |
author2 |
Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología Ecología Microbiana Molecular |
format |
Article in Journal/Newspaper |
author |
Alarcón-Schumacher, Tomás Guajardo-Leiva, Sergio Anton, Josefa Díez, Beatriz |
author_facet |
Alarcón-Schumacher, Tomás Guajardo-Leiva, Sergio Anton, Josefa Díez, Beatriz |
author_sort |
Alarcón-Schumacher, Tomás |
title |
Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula |
title_short |
Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula |
title_full |
Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula |
title_fullStr |
Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula |
title_full_unstemmed |
Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula |
title_sort |
elucidating viral communities during a phytoplankton bloom on the west antarctic peninsula |
publisher |
Frontiers Media |
publishDate |
2019 |
url |
http://hdl.handle.net/10045/91765 https://doi.org/10.3389/fmicb.2019.01014 |
long_lat |
ENVELOPE(-60.783,-60.783,-62.467,-62.467) |
geographic |
Antarctic Antarctic Peninsula Inach Southern Ocean |
geographic_facet |
Antarctic Antarctic Peninsula Inach Southern Ocean |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Southern Ocean |
op_relation |
https://doi.org/10.3389/fmicb.2019.01014 Alarcón-Schumacher T, Guajardo-Leiva S, Antón J and Díez B (2019) Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula. Front. Microbiol. 10:1014. doi:10.3389/fmicb.2019.01014 1664-302X http://hdl.handle.net/10045/91765 doi:10.3389/fmicb.2019.01014 |
op_rights |
© 2019 Alarcón-Schumacher, Guajardo-Leiva, Antón and Díez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2019.01014 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1766276396162744320 |