Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)
Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecul...
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Online Access: | http://hdl.handle.net/10045/63432 https://doi.org/10.1017/S0022149X15000218 |
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ftunivalicante:oai:rua.ua.es:10045/63432 2023-05-15T14:05:21+02:00 Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) Vidal, V. Ortiz, J. Diaz, J.I. Zafrilla, Basilio Bonete, María-José Ruiz De Ybañez, M.R. Palacios, M.J. Benzal, J. Valera, F. De La Cruz, C. Motas, M. Bautista, Vanesa Machordom, A. Barbosa, A. Universidad de Alicante. Departamento de Agroquímica y Bioquímica Biotecnología de Extremófilos (BIOTECEXTREM) 2016-03 http://hdl.handle.net/10045/63432 https://doi.org/10.1017/S0022149X15000218 eng eng Cambridge University Press http://dx.doi.org/10.1017/S0022149X15000218 Journal of Helminthology. 2016, 90(2): 214-222. doi:10.1017/S0022149X15000218 0022-149X (Print) 1475-2697 (Online) http://hdl.handle.net/10045/63432 doi:10.1017/S0022149X15000218 © Cambridge University Press 2015 info:eu-repo/semantics/restrictedAccess Spirurid nematode Stegophorus macronectes Gastrointestinal parasite Bioquímica y Biología Molecular info:eu-repo/semantics/article 2016 ftunivalicante https://doi.org/10.1017/S0022149X15000218 2020-06-05T13:12:18Z Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed. This study was funded by the Spanish Ministry of Economy and Competiveness and the European Regional Development Fund Projects CGL2004-01348, POL2006-05175, CGL2007-60369 and CTM2011-24427. V.V. was supported by a PhD grant from the Spanish Council of Scientific Research (CSIC) and the European Social Fund (JAEPre08-01053). M.J.P. was supported by a PhD grant from the Spanish Ministry of Science and Innovation (BES2005-8465). J.I.D. is a member of CONICET and is partially supported by PIP 698 (CONICET), N628 and N726 (UNLP). Article in Journal/Newspaper Antarc* Antarctic Antarctica Chinstrap penguin Deception Island Pygoscelis antarctica RUA - Repositorio Institucional de la Universidad de Alicante Antarctic Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Journal of Helminthology 90 2 214 222 |
institution |
Open Polar |
collection |
RUA - Repositorio Institucional de la Universidad de Alicante |
op_collection_id |
ftunivalicante |
language |
English |
topic |
Spirurid nematode Stegophorus macronectes Gastrointestinal parasite Bioquímica y Biología Molecular |
spellingShingle |
Spirurid nematode Stegophorus macronectes Gastrointestinal parasite Bioquímica y Biología Molecular Vidal, V. Ortiz, J. Diaz, J.I. Zafrilla, Basilio Bonete, María-José Ruiz De Ybañez, M.R. Palacios, M.J. Benzal, J. Valera, F. De La Cruz, C. Motas, M. Bautista, Vanesa Machordom, A. Barbosa, A. Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
topic_facet |
Spirurid nematode Stegophorus macronectes Gastrointestinal parasite Bioquímica y Biología Molecular |
description |
Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed. This study was funded by the Spanish Ministry of Economy and Competiveness and the European Regional Development Fund Projects CGL2004-01348, POL2006-05175, CGL2007-60369 and CTM2011-24427. V.V. was supported by a PhD grant from the Spanish Council of Scientific Research (CSIC) and the European Social Fund (JAEPre08-01053). M.J.P. was supported by a PhD grant from the Spanish Ministry of Science and Innovation (BES2005-8465). J.I.D. is a member of CONICET and is partially supported by PIP 698 (CONICET), N628 and N726 (UNLP). |
author2 |
Universidad de Alicante. Departamento de Agroquímica y Bioquímica Biotecnología de Extremófilos (BIOTECEXTREM) |
format |
Article in Journal/Newspaper |
author |
Vidal, V. Ortiz, J. Diaz, J.I. Zafrilla, Basilio Bonete, María-José Ruiz De Ybañez, M.R. Palacios, M.J. Benzal, J. Valera, F. De La Cruz, C. Motas, M. Bautista, Vanesa Machordom, A. Barbosa, A. |
author_facet |
Vidal, V. Ortiz, J. Diaz, J.I. Zafrilla, Basilio Bonete, María-José Ruiz De Ybañez, M.R. Palacios, M.J. Benzal, J. Valera, F. De La Cruz, C. Motas, M. Bautista, Vanesa Machordom, A. Barbosa, A. |
author_sort |
Vidal, V. |
title |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_short |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_full |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_fullStr |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_full_unstemmed |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_sort |
morphological, molecular and phylogenetic analyses of the spirurid nematode stegophorus macronectes (johnston & mawson, 1942) |
publisher |
Cambridge University Press |
publishDate |
2016 |
url |
http://hdl.handle.net/10045/63432 https://doi.org/10.1017/S0022149X15000218 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) |
geographic |
Antarctic Deception Island |
geographic_facet |
Antarctic Deception Island |
genre |
Antarc* Antarctic Antarctica Chinstrap penguin Deception Island Pygoscelis antarctica |
genre_facet |
Antarc* Antarctic Antarctica Chinstrap penguin Deception Island Pygoscelis antarctica |
op_relation |
http://dx.doi.org/10.1017/S0022149X15000218 Journal of Helminthology. 2016, 90(2): 214-222. doi:10.1017/S0022149X15000218 0022-149X (Print) 1475-2697 (Online) http://hdl.handle.net/10045/63432 doi:10.1017/S0022149X15000218 |
op_rights |
© Cambridge University Press 2015 info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1017/S0022149X15000218 |
container_title |
Journal of Helminthology |
container_volume |
90 |
container_issue |
2 |
container_start_page |
214 |
op_container_end_page |
222 |
_version_ |
1766277174374957056 |