New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves
The signs of climate change are undeniable, and the impact of these changes on ecosystem function heavily depends on the response of microbes that underpin the food web. Antarctic ice shelf is a massive mass of floating ice that extends from the continent into the ocean, exerting a profound influenc...
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American Society for Microbiology
2024
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Online Access: | http://hdl.handle.net/10045/142503 https://doi.org/10.1128/msphere.00073-24 |
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ftunivalicante:oai:rua.ua.es:10045/142503 2024-05-19T07:30:39+00:00 New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves Llorenç-Vicedo, Aitana Lluesma Gómez, Mónica Zeising, Ole Kleiner, Thomas Freitag, Johannes Martinez-Hernandez, Francisco Wilhelms, Frank Martinez-Garcia, Manuel Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" Ecología Microbiana Molecular 2024-04-26 http://hdl.handle.net/10045/142503 https://doi.org/10.1128/msphere.00073-24 eng eng American Society for Microbiology https://doi.org/10.1128/msphere.00073-24 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2021-125175OB-I00 mSphere. 2024. https://doi.org/10.1128/msphere.00073-24 2379-5042 http://hdl.handle.net/10045/142503 doi:10.1128/msphere.00073-24 © 2024 Llorenç Vicedo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. info:eu-repo/semantics/openAccess Antarctic Ice shelf Single-cell genomics Marine ice Microbiome Bacteria Metagenomics DNA sequencing info:eu-repo/semantics/article 2024 ftunivalicante https://doi.org/10.1128/msphere.00073-24 2024-04-30T23:33:25Z The signs of climate change are undeniable, and the impact of these changes on ecosystem function heavily depends on the response of microbes that underpin the food web. Antarctic ice shelf is a massive mass of floating ice that extends from the continent into the ocean, exerting a profound influence on global carbon cycles. Beneath Antarctic ice shelves, marine ice stores valuable genetic information, where marine microbial communities before the industrial revolution are archived. Here, in this proof-of-concept, by employing a combination of single-cell technologiesand metagenomics, we have been able to sequence frozen microbial DNA (≈300 years old) stored in the marine ice core B15 collected from the Filchnner-Ronne Ice Shelf. Metagenomic data indicated that Proteobacteria and Thaumarchaeota (e.g., Nitrosopumilus spp.), followed by Actinobacteria (e.g., Actinomarinales), were abundant. Remarkably, our data allow us to “travel to the past” and calibrate genomic and genetic evolutionary changes for ecologically relevant microbes and functions, such as Nitrosopumilus spp., preserved in the marine ice (≈300 years old) with those collected recently in seawater under an ice shelf (year 2017). The evolutionary divergence for the ammonia monooxygenase gene amoA involved in chemolithoautotrophy was about 0.88 amino acid and 2.8 nucleotide substitution rate per 100 sites in a century, while the accumulated rate of genomic SNPs was 2,467 per 1 Mb of genome and 100 years. Whether these evolutionary changes remained constant over the last 300 years or accelerated during post-industrial periods remains an open question that will be further elucidated. The authors thank the research grants funded by the Spanish Ministry of Science and Innovation and Agencia Estatal de Investigación (PID2021-125175OB-I00). Article in Journal/Newspaper Antarc* Antarctic ice core Ice Shelf Ice Shelves Ronne Ice Shelf RUA - Repositorio Institucional de la Universidad de Alicante mSphere |
institution |
Open Polar |
collection |
RUA - Repositorio Institucional de la Universidad de Alicante |
op_collection_id |
ftunivalicante |
language |
English |
topic |
Antarctic Ice shelf Single-cell genomics Marine ice Microbiome Bacteria Metagenomics DNA sequencing |
spellingShingle |
Antarctic Ice shelf Single-cell genomics Marine ice Microbiome Bacteria Metagenomics DNA sequencing Llorenç-Vicedo, Aitana Lluesma Gómez, Mónica Zeising, Ole Kleiner, Thomas Freitag, Johannes Martinez-Hernandez, Francisco Wilhelms, Frank Martinez-Garcia, Manuel New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
topic_facet |
Antarctic Ice shelf Single-cell genomics Marine ice Microbiome Bacteria Metagenomics DNA sequencing |
description |
The signs of climate change are undeniable, and the impact of these changes on ecosystem function heavily depends on the response of microbes that underpin the food web. Antarctic ice shelf is a massive mass of floating ice that extends from the continent into the ocean, exerting a profound influence on global carbon cycles. Beneath Antarctic ice shelves, marine ice stores valuable genetic information, where marine microbial communities before the industrial revolution are archived. Here, in this proof-of-concept, by employing a combination of single-cell technologiesand metagenomics, we have been able to sequence frozen microbial DNA (≈300 years old) stored in the marine ice core B15 collected from the Filchnner-Ronne Ice Shelf. Metagenomic data indicated that Proteobacteria and Thaumarchaeota (e.g., Nitrosopumilus spp.), followed by Actinobacteria (e.g., Actinomarinales), were abundant. Remarkably, our data allow us to “travel to the past” and calibrate genomic and genetic evolutionary changes for ecologically relevant microbes and functions, such as Nitrosopumilus spp., preserved in the marine ice (≈300 years old) with those collected recently in seawater under an ice shelf (year 2017). The evolutionary divergence for the ammonia monooxygenase gene amoA involved in chemolithoautotrophy was about 0.88 amino acid and 2.8 nucleotide substitution rate per 100 sites in a century, while the accumulated rate of genomic SNPs was 2,467 per 1 Mb of genome and 100 years. Whether these evolutionary changes remained constant over the last 300 years or accelerated during post-industrial periods remains an open question that will be further elucidated. The authors thank the research grants funded by the Spanish Ministry of Science and Innovation and Agencia Estatal de Investigación (PID2021-125175OB-I00). |
author2 |
Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" Ecología Microbiana Molecular |
format |
Article in Journal/Newspaper |
author |
Llorenç-Vicedo, Aitana Lluesma Gómez, Mónica Zeising, Ole Kleiner, Thomas Freitag, Johannes Martinez-Hernandez, Francisco Wilhelms, Frank Martinez-Garcia, Manuel |
author_facet |
Llorenç-Vicedo, Aitana Lluesma Gómez, Mónica Zeising, Ole Kleiner, Thomas Freitag, Johannes Martinez-Hernandez, Francisco Wilhelms, Frank Martinez-Garcia, Manuel |
author_sort |
Llorenç-Vicedo, Aitana |
title |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_short |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_full |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_fullStr |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_full_unstemmed |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves |
title_sort |
new avenues for potentially seeking microbial responses to climate change beneath antarctic ice shelves |
publisher |
American Society for Microbiology |
publishDate |
2024 |
url |
http://hdl.handle.net/10045/142503 https://doi.org/10.1128/msphere.00073-24 |
genre |
Antarc* Antarctic ice core Ice Shelf Ice Shelves Ronne Ice Shelf |
genre_facet |
Antarc* Antarctic ice core Ice Shelf Ice Shelves Ronne Ice Shelf |
op_relation |
https://doi.org/10.1128/msphere.00073-24 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2021-125175OB-I00 mSphere. 2024. https://doi.org/10.1128/msphere.00073-24 2379-5042 http://hdl.handle.net/10045/142503 doi:10.1128/msphere.00073-24 |
op_rights |
© 2024 Llorenç Vicedo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/msphere.00073-24 |
container_title |
mSphere |
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