An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic
Signatories to the Antarctic Treaty System’s Environmental Protocol are committed to preventing incursions of non-native species into Antarctica, but systematic surveillance is rare. Environmental DNA (eDNA) methods provide new opportunities for enhancing detection of non-native species and biosecur...
Published in: | Management of Biological Invasions |
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Regional Euro-Asian Biological Invasions Centre - REABIC
2023
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Online Access: | http://hdl.handle.net/10045/134555 https://doi.org/10.3391/mbi.2023.14.3.01 |
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ftunivalicante:oai:rua.ua.es:10045/134555 2023-10-25T01:30:37+02:00 An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic Clarke, Laurence J. Shaw, Justine D. Suter, Leonie Atalah, Javier Bergstrom, Dana M. Biersma, Elisabeth Machteld Convey, Peter Greve, Michelle Holland, Oakes Houghton, Melissa J. Hughes, Kevin A. Johnston, Emma L. King, Catherine K. McCarthy, Arlie H. McGaughran, Angela Pertierra, Luis R. Robinson, Sharon A. Sherman, Craig D.H. Stark, Jonathan S. Stevens, Mark I. Strugnell, Jan M. von Ammon, Ulla Wilson, Nerida G. Zaiko, Anastasija MacDonald, Anna J. Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada 2023-05-07 http://hdl.handle.net/10045/134555 https://doi.org/10.3391/mbi.2023.14.3.01 eng eng Regional Euro-Asian Biological Invasions Centre - REABIC https://doi.org/10.3391/mbi.2023.14.3.01 Management of Biological Invasions. 2023, 14(3): 379-402. https://doi.org/10.3391/mbi.2023.14.3.01 1989-8649 http://hdl.handle.net/10045/134555 doi:10.3391/mbi.2023.14.3.01 © Clarke et al. This is an open access article distributed under terms of the Creative Commons Attribution License (Attribution 4.0 International - CC BY 4.0). info:eu-repo/semantics/openAccess Biofouling Environmental DNA Non-native species Marine Southern Ocean Terrestrial info:eu-repo/semantics/article 2023 ftunivalicante https://doi.org/10.3391/mbi.2023.14.3.01 2023-09-26T23:22:42Z Signatories to the Antarctic Treaty System’s Environmental Protocol are committed to preventing incursions of non-native species into Antarctica, but systematic surveillance is rare. Environmental DNA (eDNA) methods provide new opportunities for enhancing detection of non-native species and biosecurity monitoring. To be effective for Antarctic biosecurity, eDNA tests must have appropriate sensitivity and specificity to distinguish non-native from native Antarctic species, and be fit-for-purpose. This requires knowledge of the priority risk species or taxonomic groups for which eDNA surveillance will be informative, validated eDNA assays for those species or groups, and reference DNA sequences for both target non-native and related native Antarctic species. Here, we used an expert elicitation process and decision-by-consensus approach to identify and assess priority biosecurity risks for the Australian Antarctic Program (AAP) in East Antarctica, including identifying high priority non-native species and their potential transport pathways. We determined that the priority targets for biosecurity monitoring were not individual species, but rather broader taxonomic groups such as mussels (Mytilus species), tunicates (Ascidiacea), springtails (Collembola), and grasses (Poaceae). These groups each include multiple species with high risks of introduction to and/or establishment in Antarctica. The most appropriate eDNA methods for the AAP must be capable of detecting a range of species within these high-risk groups (e.g., eDNA metabarcoding). We conclude that the most beneficial Antarctic eDNA biosecurity applications include surveillance of marine species in nearshore environments, terrestrial invertebrates, and biofouling species on vessels visiting Antarctica. An urgent need exists to identify suitable genetic markers for detecting priority species groups, establish baseline terrestrial and marine biodiversity for Antarctic stations, and develop eDNA sampling methods for detecting biofouling organisms. This work was ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Australian Antarctic Program East Antarctica Southern Ocean RUA - Repositorio Institucional de la Universidad de Alicante Antarctic East Antarctica Southern Ocean The Antarctic Management of Biological Invasions 14 3 379 402 |
institution |
Open Polar |
collection |
RUA - Repositorio Institucional de la Universidad de Alicante |
op_collection_id |
ftunivalicante |
language |
English |
topic |
Biofouling Environmental DNA Non-native species Marine Southern Ocean Terrestrial |
spellingShingle |
Biofouling Environmental DNA Non-native species Marine Southern Ocean Terrestrial Clarke, Laurence J. Shaw, Justine D. Suter, Leonie Atalah, Javier Bergstrom, Dana M. Biersma, Elisabeth Machteld Convey, Peter Greve, Michelle Holland, Oakes Houghton, Melissa J. Hughes, Kevin A. Johnston, Emma L. King, Catherine K. McCarthy, Arlie H. McGaughran, Angela Pertierra, Luis R. Robinson, Sharon A. Sherman, Craig D.H. Stark, Jonathan S. Stevens, Mark I. Strugnell, Jan M. von Ammon, Ulla Wilson, Nerida G. Zaiko, Anastasija MacDonald, Anna J. An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
topic_facet |
Biofouling Environmental DNA Non-native species Marine Southern Ocean Terrestrial |
description |
Signatories to the Antarctic Treaty System’s Environmental Protocol are committed to preventing incursions of non-native species into Antarctica, but systematic surveillance is rare. Environmental DNA (eDNA) methods provide new opportunities for enhancing detection of non-native species and biosecurity monitoring. To be effective for Antarctic biosecurity, eDNA tests must have appropriate sensitivity and specificity to distinguish non-native from native Antarctic species, and be fit-for-purpose. This requires knowledge of the priority risk species or taxonomic groups for which eDNA surveillance will be informative, validated eDNA assays for those species or groups, and reference DNA sequences for both target non-native and related native Antarctic species. Here, we used an expert elicitation process and decision-by-consensus approach to identify and assess priority biosecurity risks for the Australian Antarctic Program (AAP) in East Antarctica, including identifying high priority non-native species and their potential transport pathways. We determined that the priority targets for biosecurity monitoring were not individual species, but rather broader taxonomic groups such as mussels (Mytilus species), tunicates (Ascidiacea), springtails (Collembola), and grasses (Poaceae). These groups each include multiple species with high risks of introduction to and/or establishment in Antarctica. The most appropriate eDNA methods for the AAP must be capable of detecting a range of species within these high-risk groups (e.g., eDNA metabarcoding). We conclude that the most beneficial Antarctic eDNA biosecurity applications include surveillance of marine species in nearshore environments, terrestrial invertebrates, and biofouling species on vessels visiting Antarctica. An urgent need exists to identify suitable genetic markers for detecting priority species groups, establish baseline terrestrial and marine biodiversity for Antarctic stations, and develop eDNA sampling methods for detecting biofouling organisms. This work was ... |
author2 |
Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada |
format |
Article in Journal/Newspaper |
author |
Clarke, Laurence J. Shaw, Justine D. Suter, Leonie Atalah, Javier Bergstrom, Dana M. Biersma, Elisabeth Machteld Convey, Peter Greve, Michelle Holland, Oakes Houghton, Melissa J. Hughes, Kevin A. Johnston, Emma L. King, Catherine K. McCarthy, Arlie H. McGaughran, Angela Pertierra, Luis R. Robinson, Sharon A. Sherman, Craig D.H. Stark, Jonathan S. Stevens, Mark I. Strugnell, Jan M. von Ammon, Ulla Wilson, Nerida G. Zaiko, Anastasija MacDonald, Anna J. |
author_facet |
Clarke, Laurence J. Shaw, Justine D. Suter, Leonie Atalah, Javier Bergstrom, Dana M. Biersma, Elisabeth Machteld Convey, Peter Greve, Michelle Holland, Oakes Houghton, Melissa J. Hughes, Kevin A. Johnston, Emma L. King, Catherine K. McCarthy, Arlie H. McGaughran, Angela Pertierra, Luis R. Robinson, Sharon A. Sherman, Craig D.H. Stark, Jonathan S. Stevens, Mark I. Strugnell, Jan M. von Ammon, Ulla Wilson, Nerida G. Zaiko, Anastasija MacDonald, Anna J. |
author_sort |
Clarke, Laurence J. |
title |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_short |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_full |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_fullStr |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_full_unstemmed |
An expert-driven framework for applying eDNA tools to improve biosecurity in the Antarctic |
title_sort |
expert-driven framework for applying edna tools to improve biosecurity in the antarctic |
publisher |
Regional Euro-Asian Biological Invasions Centre - REABIC |
publishDate |
2023 |
url |
http://hdl.handle.net/10045/134555 https://doi.org/10.3391/mbi.2023.14.3.01 |
geographic |
Antarctic East Antarctica Southern Ocean The Antarctic |
geographic_facet |
Antarctic East Antarctica Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctica Australian Antarctic Program East Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Australian Antarctic Program East Antarctica Southern Ocean |
op_relation |
https://doi.org/10.3391/mbi.2023.14.3.01 Management of Biological Invasions. 2023, 14(3): 379-402. https://doi.org/10.3391/mbi.2023.14.3.01 1989-8649 http://hdl.handle.net/10045/134555 doi:10.3391/mbi.2023.14.3.01 |
op_rights |
© Clarke et al. This is an open access article distributed under terms of the Creative Commons Attribution License (Attribution 4.0 International - CC BY 4.0). info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3391/mbi.2023.14.3.01 |
container_title |
Management of Biological Invasions |
container_volume |
14 |
container_issue |
3 |
container_start_page |
379 |
op_container_end_page |
402 |
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1780740941218316288 |