Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1 mu var): Mortality, viral load and detection of viral transcripts by in situ hybridization
Ostreid herpesvirus 1 (OsHV-1) infections have been reported in several bivalve species. Mortality of Pacific oyster Crassostrea gigas spat has increased considerably in Europe since 2008 linked to the spread of a variant of OsHV-1 called var. In the present study we demonstrated that O. edulis juve...
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Online Access: | http://hdl.handle.net/10400.1/9432 https://doi.org/10.1016/j.virusres.2016.01.023 |
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ftunivalgarve:oai:sapientia.ualg.pt:10400.1/9432 2023-05-15T15:58:41+02:00 Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1 mu var): Mortality, viral load and detection of viral transcripts by in situ hybridization Sanmartin, Monserrat Lopez Power, Deborah de la Herran, Roberto Navas, Jose I. Batista, Frederico 2016-06 http://hdl.handle.net/10400.1/9432 https://doi.org/10.1016/j.virusres.2016.01.023 eng eng Elsevier WOS:000375498900008 0168-1702 AUT: DPO00386; http://hdl.handle.net/10400.1/9432 doi:10.1016/j.virusres.2016.01.023 restrictedAccess article 2016 ftunivalgarve https://doi.org/10.1016/j.virusres.2016.01.023 2022-05-30T08:47:36Z Ostreid herpesvirus 1 (OsHV-1) infections have been reported in several bivalve species. Mortality of Pacific oyster Crassostrea gigas spat has increased considerably in Europe since 2008 linked to the spread of a variant of OsHV-1 called var. In the present study we demonstrated that O. edulis juveniles can be infected by OsHV-1 mu var when administered as an intramuscular injection. Mortality in the oysters injected with OsHV-1 mu var was first detected 4 days after injection and reached 25% mortality at day 10. Moreover, the high viral load observed and the detection of viral transcripts by in situ hybridization in several tissues of dying oysters suggested that OsHV-1 mu var was the cause of mortality in the O. edulis juveniles. This is therefore the first study to provide evidence about the pathogenicity of OsHV-1 var in a species that does not belong to the Crassostrea genus. Additionally, we present a novel method to detect OsHV-1 transcripts in infected individuals' using in situ hybridization. (C) 2016 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Universidade do Algarve: Sapienta Pacific Virus Research 217 55 62 |
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Open Polar |
collection |
Universidade do Algarve: Sapienta |
op_collection_id |
ftunivalgarve |
language |
English |
description |
Ostreid herpesvirus 1 (OsHV-1) infections have been reported in several bivalve species. Mortality of Pacific oyster Crassostrea gigas spat has increased considerably in Europe since 2008 linked to the spread of a variant of OsHV-1 called var. In the present study we demonstrated that O. edulis juveniles can be infected by OsHV-1 mu var when administered as an intramuscular injection. Mortality in the oysters injected with OsHV-1 mu var was first detected 4 days after injection and reached 25% mortality at day 10. Moreover, the high viral load observed and the detection of viral transcripts by in situ hybridization in several tissues of dying oysters suggested that OsHV-1 mu var was the cause of mortality in the O. edulis juveniles. This is therefore the first study to provide evidence about the pathogenicity of OsHV-1 var in a species that does not belong to the Crassostrea genus. Additionally, we present a novel method to detect OsHV-1 transcripts in infected individuals' using in situ hybridization. (C) 2016 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Sanmartin, Monserrat Lopez Power, Deborah de la Herran, Roberto Navas, Jose I. Batista, Frederico |
spellingShingle |
Sanmartin, Monserrat Lopez Power, Deborah de la Herran, Roberto Navas, Jose I. Batista, Frederico Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1 mu var): Mortality, viral load and detection of viral transcripts by in situ hybridization |
author_facet |
Sanmartin, Monserrat Lopez Power, Deborah de la Herran, Roberto Navas, Jose I. Batista, Frederico |
author_sort |
Sanmartin, Monserrat Lopez |
title |
Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1 mu var): Mortality, viral load and detection of viral transcripts by in situ hybridization |
title_short |
Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1 mu var): Mortality, viral load and detection of viral transcripts by in situ hybridization |
title_full |
Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1 mu var): Mortality, viral load and detection of viral transcripts by in situ hybridization |
title_fullStr |
Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1 mu var): Mortality, viral load and detection of viral transcripts by in situ hybridization |
title_full_unstemmed |
Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1 mu var): Mortality, viral load and detection of viral transcripts by in situ hybridization |
title_sort |
experimental infection of european flat oyster ostrea edulis with ostreid herpesvirus 1 microvar (oshv-1 mu var): mortality, viral load and detection of viral transcripts by in situ hybridization |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://hdl.handle.net/10400.1/9432 https://doi.org/10.1016/j.virusres.2016.01.023 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
WOS:000375498900008 0168-1702 AUT: DPO00386; http://hdl.handle.net/10400.1/9432 doi:10.1016/j.virusres.2016.01.023 |
op_rights |
restrictedAccess |
op_doi |
https://doi.org/10.1016/j.virusres.2016.01.023 |
container_title |
Virus Research |
container_volume |
217 |
container_start_page |
55 |
op_container_end_page |
62 |
_version_ |
1766394453953609728 |