Molecular, morphological and chemical diversity of two new species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov.
The current study focuses on the biological diversity of two strains of Antarctic diatoms (strains IMA082A and IMA088A) collected and isolated from the Ross Sea (Antarctica) during the XXXIV Italian Antarctic Expedition. Both species presented the typical morphological characters of the genus Craspe...
Published in: | Journal of Marine Science and Engineering |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI
2022
|
Subjects: | |
Online Access: | http://hdl.handle.net/10400.1/18500 https://doi.org/10.3390/jmse10111656 |
id |
ftunivalgarve:oai:sapientia.ualg.pt:10400.1/18500 |
---|---|
record_format |
openpolar |
spelling |
ftunivalgarve:oai:sapientia.ualg.pt:10400.1/18500 2023-05-15T14:03:09+02:00 Molecular, morphological and chemical diversity of two new species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. Trentin, Riccardo Moschin, Emanuela Lopes, André Duarte Schiaparelli, Stefano L, Custódio Moro, Isabella 2022-11-10T14:28:01Z http://hdl.handle.net/10400.1/18500 https://doi.org/10.3390/jmse10111656 eng eng MDPI info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00425%2F2017%2FCP1391%2FCT0001/PT Journal of Marine Science and Engineering 10 (11): 1656 (2022) http://hdl.handle.net/10400.1/18500 doi:10.3390/jmse10111656 2077-1312 openAccess http://creativecommons.org/licenses/by/4.0/ CC-BY Antarctica Biodiversity Ross Sea Diatoms Craspedostauros Craspedostauros ineffabilis sp. nov Craspedostauros zucchellii sp. nov Morphology Molecular phylogeny Chemical diversity article 2022 ftunivalgarve https://doi.org/10.3390/jmse10111656 2022-11-16T01:01:59Z The current study focuses on the biological diversity of two strains of Antarctic diatoms (strains IMA082A and IMA088A) collected and isolated from the Ross Sea (Antarctica) during the XXXIV Italian Antarctic Expedition. Both species presented the typical morphological characters of the genus Craspedostauros: cribrate areolae, two “fore-and-aft” chloroplasts and a narrow “stauros”. This classification is congruent with the molecular phylogeny based on the concatenated 18S rDNArbcL-psbC alignment, which showed that these algae formed a monophyletic lineage including six taxonomically accepted species of Craspedostauros. Since the study of the evolution of this genus and of others raphe-bearing diatoms with a “stauros” is particularly challenging and their phylogeny is still debated, we tested alternative tree topologies to evaluate the relationships among these taxa. The metabolic fingerprinting approach was implemented for the assessment of the chemical diversity of IMA082A and IMA088A. In conclusion, combining (1) traditional morphological features used in diatoms identification, (2) phylogenetic analyses of the small subunit rDNA (18S rDNA), rbcL and psbC genes, and (3) metabolic fingerprint, we described the strains IMA082A and IMA088A as Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. as new species, respectively info:eu-repo/semantics/publishedVersion Article in Journal/Newspaper Antarc* Antarctic Antarctica Ross Sea Universidade do Algarve: Sapienta Antarctic Ross Sea Journal of Marine Science and Engineering 10 11 1656 |
institution |
Open Polar |
collection |
Universidade do Algarve: Sapienta |
op_collection_id |
ftunivalgarve |
language |
English |
topic |
Antarctica Biodiversity Ross Sea Diatoms Craspedostauros Craspedostauros ineffabilis sp. nov Craspedostauros zucchellii sp. nov Morphology Molecular phylogeny Chemical diversity |
spellingShingle |
Antarctica Biodiversity Ross Sea Diatoms Craspedostauros Craspedostauros ineffabilis sp. nov Craspedostauros zucchellii sp. nov Morphology Molecular phylogeny Chemical diversity Trentin, Riccardo Moschin, Emanuela Lopes, André Duarte Schiaparelli, Stefano L, Custódio Moro, Isabella Molecular, morphological and chemical diversity of two new species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
topic_facet |
Antarctica Biodiversity Ross Sea Diatoms Craspedostauros Craspedostauros ineffabilis sp. nov Craspedostauros zucchellii sp. nov Morphology Molecular phylogeny Chemical diversity |
description |
The current study focuses on the biological diversity of two strains of Antarctic diatoms (strains IMA082A and IMA088A) collected and isolated from the Ross Sea (Antarctica) during the XXXIV Italian Antarctic Expedition. Both species presented the typical morphological characters of the genus Craspedostauros: cribrate areolae, two “fore-and-aft” chloroplasts and a narrow “stauros”. This classification is congruent with the molecular phylogeny based on the concatenated 18S rDNArbcL-psbC alignment, which showed that these algae formed a monophyletic lineage including six taxonomically accepted species of Craspedostauros. Since the study of the evolution of this genus and of others raphe-bearing diatoms with a “stauros” is particularly challenging and their phylogeny is still debated, we tested alternative tree topologies to evaluate the relationships among these taxa. The metabolic fingerprinting approach was implemented for the assessment of the chemical diversity of IMA082A and IMA088A. In conclusion, combining (1) traditional morphological features used in diatoms identification, (2) phylogenetic analyses of the small subunit rDNA (18S rDNA), rbcL and psbC genes, and (3) metabolic fingerprint, we described the strains IMA082A and IMA088A as Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. as new species, respectively info:eu-repo/semantics/publishedVersion |
format |
Article in Journal/Newspaper |
author |
Trentin, Riccardo Moschin, Emanuela Lopes, André Duarte Schiaparelli, Stefano L, Custódio Moro, Isabella |
author_facet |
Trentin, Riccardo Moschin, Emanuela Lopes, André Duarte Schiaparelli, Stefano L, Custódio Moro, Isabella |
author_sort |
Trentin, Riccardo |
title |
Molecular, morphological and chemical diversity of two new species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_short |
Molecular, morphological and chemical diversity of two new species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_full |
Molecular, morphological and chemical diversity of two new species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_fullStr |
Molecular, morphological and chemical diversity of two new species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_full_unstemmed |
Molecular, morphological and chemical diversity of two new species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_sort |
molecular, morphological and chemical diversity of two new species of antarctic diatoms, craspedostauros ineffabilis sp. nov. and craspedostauros zucchellii sp. nov. |
publisher |
MDPI |
publishDate |
2022 |
url |
http://hdl.handle.net/10400.1/18500 https://doi.org/10.3390/jmse10111656 |
geographic |
Antarctic Ross Sea |
geographic_facet |
Antarctic Ross Sea |
genre |
Antarc* Antarctic Antarctica Ross Sea |
genre_facet |
Antarc* Antarctic Antarctica Ross Sea |
op_relation |
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00425%2F2017%2FCP1391%2FCT0001/PT Journal of Marine Science and Engineering 10 (11): 1656 (2022) http://hdl.handle.net/10400.1/18500 doi:10.3390/jmse10111656 2077-1312 |
op_rights |
openAccess http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/jmse10111656 |
container_title |
Journal of Marine Science and Engineering |
container_volume |
10 |
container_issue |
11 |
container_start_page |
1656 |
_version_ |
1766273694898847744 |