Extracellular multi-unit recording from the olfactory nerve of teleosts
Recent studies have shown that ocean acidification affects olfactory-driven behavior in fish. This may be due in part to a reduction in olfactory sensitivity in high PCO2/low pH water. To assess the effects of ocean acidification, or olfactory sensitivity in marine fish in general, we propose that e...
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ftunivalgarve:oai:sapientia.ualg.pt:10400.1/14946 2023-05-15T17:50:10+02:00 Extracellular multi-unit recording from the olfactory nerve of teleosts Hubbard, Peter Velez, Zélia 2020 http://hdl.handle.net/10400.1/14946 https://doi.org/10.3791/60962 eng eng MyJove Corporation 1940-087X http://hdl.handle.net/10400.1/14946 doi:10.3791/60962 openAccess http://creativecommons.org/licenses/by/4.0/ CC-BY Animals Carbon dioxide Electrophysiological phenomena Extracellular space Fishes Glutamine Hydrogen-ion concentration Leucine Olfactory nerve Seawater Smell Software article 2020 ftunivalgarve https://doi.org/10.3791/60962 2022-05-30T08:49:27Z Recent studies have shown that ocean acidification affects olfactory-driven behavior in fish. This may be due in part to a reduction in olfactory sensitivity in high PCO2/low pH water. To assess the effects of ocean acidification, or olfactory sensitivity in marine fish in general, we propose that extracellular multi-unit recording from the olfactory nerve is the method of choice. Although invasive, it is sensitive, robust, reproducible and independent of external salinity (unlike the electro-olfactogram [EOG], for example). Furthermore, it records a primary sensory input into the CNS, prior to any central processing. We show that this method can show a reduction in olfactory sensitivity that is both temporary and odorant-dependent, using a range of amino acids to construct concentration-response curves and calculate the thresholds of detection. FCT: PTDC/BIA-BMA/30262/2017/ DL57/2016/CP1361/CT0041 info:eu-repo/semantics/publishedVersion Article in Journal/Newspaper Ocean acidification Universidade do Algarve: Sapienta Bia ENVELOPE(22.891,22.891,70.317,70.317) Journal of Visualized Experiments 164 |
institution |
Open Polar |
collection |
Universidade do Algarve: Sapienta |
op_collection_id |
ftunivalgarve |
language |
English |
topic |
Animals Carbon dioxide Electrophysiological phenomena Extracellular space Fishes Glutamine Hydrogen-ion concentration Leucine Olfactory nerve Seawater Smell Software |
spellingShingle |
Animals Carbon dioxide Electrophysiological phenomena Extracellular space Fishes Glutamine Hydrogen-ion concentration Leucine Olfactory nerve Seawater Smell Software Hubbard, Peter Velez, Zélia Extracellular multi-unit recording from the olfactory nerve of teleosts |
topic_facet |
Animals Carbon dioxide Electrophysiological phenomena Extracellular space Fishes Glutamine Hydrogen-ion concentration Leucine Olfactory nerve Seawater Smell Software |
description |
Recent studies have shown that ocean acidification affects olfactory-driven behavior in fish. This may be due in part to a reduction in olfactory sensitivity in high PCO2/low pH water. To assess the effects of ocean acidification, or olfactory sensitivity in marine fish in general, we propose that extracellular multi-unit recording from the olfactory nerve is the method of choice. Although invasive, it is sensitive, robust, reproducible and independent of external salinity (unlike the electro-olfactogram [EOG], for example). Furthermore, it records a primary sensory input into the CNS, prior to any central processing. We show that this method can show a reduction in olfactory sensitivity that is both temporary and odorant-dependent, using a range of amino acids to construct concentration-response curves and calculate the thresholds of detection. FCT: PTDC/BIA-BMA/30262/2017/ DL57/2016/CP1361/CT0041 info:eu-repo/semantics/publishedVersion |
format |
Article in Journal/Newspaper |
author |
Hubbard, Peter Velez, Zélia |
author_facet |
Hubbard, Peter Velez, Zélia |
author_sort |
Hubbard, Peter |
title |
Extracellular multi-unit recording from the olfactory nerve of teleosts |
title_short |
Extracellular multi-unit recording from the olfactory nerve of teleosts |
title_full |
Extracellular multi-unit recording from the olfactory nerve of teleosts |
title_fullStr |
Extracellular multi-unit recording from the olfactory nerve of teleosts |
title_full_unstemmed |
Extracellular multi-unit recording from the olfactory nerve of teleosts |
title_sort |
extracellular multi-unit recording from the olfactory nerve of teleosts |
publisher |
MyJove Corporation |
publishDate |
2020 |
url |
http://hdl.handle.net/10400.1/14946 https://doi.org/10.3791/60962 |
long_lat |
ENVELOPE(22.891,22.891,70.317,70.317) |
geographic |
Bia |
geographic_facet |
Bia |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
1940-087X http://hdl.handle.net/10400.1/14946 doi:10.3791/60962 |
op_rights |
openAccess http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3791/60962 |
container_title |
Journal of Visualized Experiments |
container_issue |
164 |
_version_ |
1766156811055923200 |