Extracellular multi-unit recording from the olfactory nerve of teleosts
Recent studies have shown that ocean acidification affects olfactory-driven behavior in fish. This may be due in part to a reduction in olfactory sensitivity in high PCO2/low pH water. To assess the effects of ocean acidification, or olfactory sensitivity in marine fish in general, we propose that e...
Published in: | Journal of Visualized Experiments |
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Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MyJove Corporation
2020
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Subjects: | |
Online Access: | http://hdl.handle.net/10400.1/14946 https://doi.org/10.3791/60962 |
Summary: | Recent studies have shown that ocean acidification affects olfactory-driven behavior in fish. This may be due in part to a reduction in olfactory sensitivity in high PCO2/low pH water. To assess the effects of ocean acidification, or olfactory sensitivity in marine fish in general, we propose that extracellular multi-unit recording from the olfactory nerve is the method of choice. Although invasive, it is sensitive, robust, reproducible and independent of external salinity (unlike the electro-olfactogram [EOG], for example). Furthermore, it records a primary sensory input into the CNS, prior to any central processing. We show that this method can show a reduction in olfactory sensitivity that is both temporary and odorant-dependent, using a range of amino acids to construct concentration-response curves and calculate the thresholds of detection. FCT: PTDC/BIA-BMA/30262/2017/ DL57/2016/CP1361/CT0041 info:eu-repo/semantics/publishedVersion |
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