The calcitonin-like system is an ancient regulatory system of biomineralization
Biomineralization is the process by which living organisms acquired the capacity to accumulate minerals in tissues. Shells are the biomineralized exoskeleton of marine molluscs produced by the mantle but factors that regulate mantle shell building are still enigmatic. This study sought to identify c...
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ftunivalgarve:oai:sapientia.ualg.pt:10400.1/13965 2023-05-15T17:51:36+02:00 The calcitonin-like system is an ancient regulatory system of biomineralization Cardoso, João CR Félix, Rute C. Ferreira, Vinícius Peng, Maoxiao Zhang, Xushuai Power, Deborah 2020 http://hdl.handle.net/10400.1/13965 https://doi.org/10.1038/s41598-020-64118-w eng eng 2045-2322 http://hdl.handle.net/10400.1/13965 doi:10.1038/s41598-020-64118-w openAccess Gene-related peptide Proteins Resistant acid-phosphatase Bone Oyster pinctada-fucata Receptors Ocean acidification Teleost fish article 2020 ftunivalgarve https://doi.org/10.1038/s41598-020-64118-w 2022-05-30T08:49:08Z Biomineralization is the process by which living organisms acquired the capacity to accumulate minerals in tissues. Shells are the biomineralized exoskeleton of marine molluscs produced by the mantle but factors that regulate mantle shell building are still enigmatic. This study sought to identify candidate regulatory factors of molluscan shell mineralization and targeted family B G-protein coupled receptors (GPCRs) and ligands that include calcium regulatory factors in vertebrates, such as calcitonin (CALC). In molluscs, CALC receptor (CALCR) number was variable and arose through lineage and species-specific duplications. The Mediterranean mussel (Mytilus galloprovincialis) mantle transcriptome expresses six CALCR-like and two CALC-precursors encoding four putative mature peptides. Mussel CALCR-like are activated in vitro by vertebrate CALC but only receptor CALCRIIc is activated by the mussel CALCIIa peptide (EC50 = 2.6 ×10-5 M). Ex-vivo incubations of mantle edge tissue and mantle cells with CALCIIa revealed they accumulated significantly more calcium than untreated tissue and cells. Mussel CALCIIa also significantly decreased mantle acid phosphatase activity, which is associated with shell remodelling. Our data indicate the CALC-like system as candidate regulatory factors of shell mineralization. The identification of the CALC system from molluscs to vertebrates suggests it is an ancient and conserved calcium regulatory system of mineralization. CCMAR UIDB/04326/2020; CRESC Algarve 2020 and COMPETE 2020 through project EMBRC.PT ALG-01-0145-FEDER-022121. FCT: DL57/2016/CP1361/CT0020 info:eu-repo/semantics/publishedVersion Article in Journal/Newspaper Ocean acidification Universidade do Algarve: Sapienta Scientific Reports 10 1 |
institution |
Open Polar |
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Universidade do Algarve: Sapienta |
op_collection_id |
ftunivalgarve |
language |
English |
topic |
Gene-related peptide Proteins Resistant acid-phosphatase Bone Oyster pinctada-fucata Receptors Ocean acidification Teleost fish |
spellingShingle |
Gene-related peptide Proteins Resistant acid-phosphatase Bone Oyster pinctada-fucata Receptors Ocean acidification Teleost fish Cardoso, João CR Félix, Rute C. Ferreira, Vinícius Peng, Maoxiao Zhang, Xushuai Power, Deborah The calcitonin-like system is an ancient regulatory system of biomineralization |
topic_facet |
Gene-related peptide Proteins Resistant acid-phosphatase Bone Oyster pinctada-fucata Receptors Ocean acidification Teleost fish |
description |
Biomineralization is the process by which living organisms acquired the capacity to accumulate minerals in tissues. Shells are the biomineralized exoskeleton of marine molluscs produced by the mantle but factors that regulate mantle shell building are still enigmatic. This study sought to identify candidate regulatory factors of molluscan shell mineralization and targeted family B G-protein coupled receptors (GPCRs) and ligands that include calcium regulatory factors in vertebrates, such as calcitonin (CALC). In molluscs, CALC receptor (CALCR) number was variable and arose through lineage and species-specific duplications. The Mediterranean mussel (Mytilus galloprovincialis) mantle transcriptome expresses six CALCR-like and two CALC-precursors encoding four putative mature peptides. Mussel CALCR-like are activated in vitro by vertebrate CALC but only receptor CALCRIIc is activated by the mussel CALCIIa peptide (EC50 = 2.6 ×10-5 M). Ex-vivo incubations of mantle edge tissue and mantle cells with CALCIIa revealed they accumulated significantly more calcium than untreated tissue and cells. Mussel CALCIIa also significantly decreased mantle acid phosphatase activity, which is associated with shell remodelling. Our data indicate the CALC-like system as candidate regulatory factors of shell mineralization. The identification of the CALC system from molluscs to vertebrates suggests it is an ancient and conserved calcium regulatory system of mineralization. CCMAR UIDB/04326/2020; CRESC Algarve 2020 and COMPETE 2020 through project EMBRC.PT ALG-01-0145-FEDER-022121. FCT: DL57/2016/CP1361/CT0020 info:eu-repo/semantics/publishedVersion |
format |
Article in Journal/Newspaper |
author |
Cardoso, João CR Félix, Rute C. Ferreira, Vinícius Peng, Maoxiao Zhang, Xushuai Power, Deborah |
author_facet |
Cardoso, João CR Félix, Rute C. Ferreira, Vinícius Peng, Maoxiao Zhang, Xushuai Power, Deborah |
author_sort |
Cardoso, João CR |
title |
The calcitonin-like system is an ancient regulatory system of biomineralization |
title_short |
The calcitonin-like system is an ancient regulatory system of biomineralization |
title_full |
The calcitonin-like system is an ancient regulatory system of biomineralization |
title_fullStr |
The calcitonin-like system is an ancient regulatory system of biomineralization |
title_full_unstemmed |
The calcitonin-like system is an ancient regulatory system of biomineralization |
title_sort |
calcitonin-like system is an ancient regulatory system of biomineralization |
publishDate |
2020 |
url |
http://hdl.handle.net/10400.1/13965 https://doi.org/10.1038/s41598-020-64118-w |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
2045-2322 http://hdl.handle.net/10400.1/13965 doi:10.1038/s41598-020-64118-w |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1038/s41598-020-64118-w |
container_title |
Scientific Reports |
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10 |
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1 |
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1766158804308721664 |