Differential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofen
Pharmaceuticals, such as anti-inflammatory nonsteroidal drugs, are frequently detected in aquatic ecosystems. Studies about the effects of these substances in nontarget organisms, such as bivalves, are relevant. The aim of this study was to evaluate the effects on antioxidant status caused by ibupro...
Published in: | Environmental Science and Pollution Research |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | http://hdl.handle.net/10400.1/13739 https://doi.org/10.1007/s11356-014-4023-0 |
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ftunivalgarve:oai:sapientia.ualg.pt:10400.1/13739 2023-05-15T15:58:22+02:00 Differential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofen Serrano, Miguel A. S. Gonzalez-Rey, Maria Mattos, Jacó J. Flores-Nunes, Fabrício Mello, Álvaro C. P. Zacchi, Flávia L. Piazza, Clei E. Siebert, Marília N. Piazza, Rômi S. Alvarez-Muñoz, Diana Rodriguez-Mozaz, Sara Barceló, Damià Bebianno, Maria João Gomes, Carlos H. A. M. Melo, Cláudio M. R. Bainy, Afonso C. D. 2015 http://hdl.handle.net/10400.1/13739 https://doi.org/10.1007/s11356-014-4023-0 eng eng Springer 0944-1344 http://hdl.handle.net/10400.1/13739 doi:10.1007/s11356-014-4023-0 restrictedAccess http://creativecommons.org/licenses/by/4.0/ CC-BY Animals Antioxidants Cell survival Crassostrea Cytotoxins Dose-response relationship Gills Hemocytes Ibuprofen Transcription Water pollutants Ecotoxicology Chemical Drug article 2015 ftunivalgarve https://doi.org/10.1007/s11356-014-4023-0 2022-05-30T08:49:04Z Pharmaceuticals, such as anti-inflammatory nonsteroidal drugs, are frequently detected in aquatic ecosystems. Studies about the effects of these substances in nontarget organisms, such as bivalves, are relevant. The aim of this study was to evaluate the effects on antioxidant status caused by ibuprofen (IBU) in oysters Crassostrea gigas exposed for 1, 4, and 7 days at concentrations 1 and 100 μg L(-1). Levels of IBU in tissues of oysters, as well as cell viability of hemocytes, were measured. The transcription of cytochrome P450 genes (CYP2AU2, CYP356A1, CYP3071A1, CYP30C1), glutathione S-transferase isoforms (GST-ω-like and GST-π-like), cyclooxygenase-like (COX-like), fatty acid binding protein-like (FABP-like), caspase-like, heat shock protein-like (HSP70-like), catalase-like (CAT-like), and the activity of catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione S-transferase (GST) were also evaluated in the gills of oysters. The highest levels of IBU were observed in animals exposed to 100 μg L(-1). A significant upregulation of CYP2AU1, CYP356A1, CYP3071A1, GST-ω-like, GST-π-like, COX-like, and FABP-like was observed in oysters exposed to IBU under different experimental conditions. Oysters exposed to 1 μg L(-1) for 7 days showed a significantly higher transcription of CYP2AU2, CYP356A1, CYP3071A1, GST-ω-like, and GST-π-like but lower GR activity. In conclusion, C. gigas exposed to environmentally relevant concentrations of IBU (1 μg L(-1)) exhibited increased transcription of certain genes and alterations on antioxidant and auxiliary enzymes, which could, in the the long term, cause damages to exposed organisms. info:eu-repo/semantics/publishedVersion Article in Journal/Newspaper Crassostrea gigas Pacific oyster Universidade do Algarve: Sapienta Pacific Environmental Science and Pollution Research 22 22 17375 17385 |
institution |
Open Polar |
collection |
Universidade do Algarve: Sapienta |
op_collection_id |
ftunivalgarve |
language |
English |
topic |
Animals Antioxidants Cell survival Crassostrea Cytotoxins Dose-response relationship Gills Hemocytes Ibuprofen Transcription Water pollutants Ecotoxicology Chemical Drug |
spellingShingle |
Animals Antioxidants Cell survival Crassostrea Cytotoxins Dose-response relationship Gills Hemocytes Ibuprofen Transcription Water pollutants Ecotoxicology Chemical Drug Serrano, Miguel A. S. Gonzalez-Rey, Maria Mattos, Jacó J. Flores-Nunes, Fabrício Mello, Álvaro C. P. Zacchi, Flávia L. Piazza, Clei E. Siebert, Marília N. Piazza, Rômi S. Alvarez-Muñoz, Diana Rodriguez-Mozaz, Sara Barceló, Damià Bebianno, Maria João Gomes, Carlos H. A. M. Melo, Cláudio M. R. Bainy, Afonso C. D. Differential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofen |
topic_facet |
Animals Antioxidants Cell survival Crassostrea Cytotoxins Dose-response relationship Gills Hemocytes Ibuprofen Transcription Water pollutants Ecotoxicology Chemical Drug |
description |
Pharmaceuticals, such as anti-inflammatory nonsteroidal drugs, are frequently detected in aquatic ecosystems. Studies about the effects of these substances in nontarget organisms, such as bivalves, are relevant. The aim of this study was to evaluate the effects on antioxidant status caused by ibuprofen (IBU) in oysters Crassostrea gigas exposed for 1, 4, and 7 days at concentrations 1 and 100 μg L(-1). Levels of IBU in tissues of oysters, as well as cell viability of hemocytes, were measured. The transcription of cytochrome P450 genes (CYP2AU2, CYP356A1, CYP3071A1, CYP30C1), glutathione S-transferase isoforms (GST-ω-like and GST-π-like), cyclooxygenase-like (COX-like), fatty acid binding protein-like (FABP-like), caspase-like, heat shock protein-like (HSP70-like), catalase-like (CAT-like), and the activity of catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione S-transferase (GST) were also evaluated in the gills of oysters. The highest levels of IBU were observed in animals exposed to 100 μg L(-1). A significant upregulation of CYP2AU1, CYP356A1, CYP3071A1, GST-ω-like, GST-π-like, COX-like, and FABP-like was observed in oysters exposed to IBU under different experimental conditions. Oysters exposed to 1 μg L(-1) for 7 days showed a significantly higher transcription of CYP2AU2, CYP356A1, CYP3071A1, GST-ω-like, and GST-π-like but lower GR activity. In conclusion, C. gigas exposed to environmentally relevant concentrations of IBU (1 μg L(-1)) exhibited increased transcription of certain genes and alterations on antioxidant and auxiliary enzymes, which could, in the the long term, cause damages to exposed organisms. info:eu-repo/semantics/publishedVersion |
format |
Article in Journal/Newspaper |
author |
Serrano, Miguel A. S. Gonzalez-Rey, Maria Mattos, Jacó J. Flores-Nunes, Fabrício Mello, Álvaro C. P. Zacchi, Flávia L. Piazza, Clei E. Siebert, Marília N. Piazza, Rômi S. Alvarez-Muñoz, Diana Rodriguez-Mozaz, Sara Barceló, Damià Bebianno, Maria João Gomes, Carlos H. A. M. Melo, Cláudio M. R. Bainy, Afonso C. D. |
author_facet |
Serrano, Miguel A. S. Gonzalez-Rey, Maria Mattos, Jacó J. Flores-Nunes, Fabrício Mello, Álvaro C. P. Zacchi, Flávia L. Piazza, Clei E. Siebert, Marília N. Piazza, Rômi S. Alvarez-Muñoz, Diana Rodriguez-Mozaz, Sara Barceló, Damià Bebianno, Maria João Gomes, Carlos H. A. M. Melo, Cláudio M. R. Bainy, Afonso C. D. |
author_sort |
Serrano, Miguel A. S. |
title |
Differential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofen |
title_short |
Differential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofen |
title_full |
Differential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofen |
title_fullStr |
Differential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofen |
title_full_unstemmed |
Differential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofen |
title_sort |
differential gene transcription, biochemical responses, and cytotoxicity assessment in pacific oyster crassostrea gigas exposed to ibuprofen |
publisher |
Springer |
publishDate |
2015 |
url |
http://hdl.handle.net/10400.1/13739 https://doi.org/10.1007/s11356-014-4023-0 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
0944-1344 http://hdl.handle.net/10400.1/13739 doi:10.1007/s11356-014-4023-0 |
op_rights |
restrictedAccess http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s11356-014-4023-0 |
container_title |
Environmental Science and Pollution Research |
container_volume |
22 |
container_issue |
22 |
container_start_page |
17375 |
op_container_end_page |
17385 |
_version_ |
1766394100965179392 |