Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification
Here, we report the first use of massive-scale RNA-sequencing to explore seagrass response to CO2-driven ocean acidification (OA). Large-scale gene expression changes in the seagrass Cymodocea nodosa occurred at CO2 levels projected by the end of the century. C. nodosa transcriptome was obtained usi...
Published in: | Molecular Ecology |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2017
|
Subjects: | |
Online Access: | http://hdl.handle.net/10400.1/13055 https://doi.org/10.1111/mec.14204 |
id |
ftunivalgarve:oai:sapientia.ualg.pt:10400.1/13055 |
---|---|
record_format |
openpolar |
spelling |
ftunivalgarve:oai:sapientia.ualg.pt:10400.1/13055 2023-05-15T17:50:14+02:00 Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification Ruocco, Miriam Musacchia, Francesco Olivé, Irene Costa, Monya Barrote, Isabel Santos, Rui Sanges, Remo Procaccini, Gabriele Silva, João 2017-08 http://hdl.handle.net/10400.1/13055 https://doi.org/10.1111/mec.14204 eng eng Wiley info:eu-repo/grantAgreement/FCT/3599-PPCDT/130156/PT 0962-1083 http://hdl.handle.net/10400.1/13055 doi:10.1111/mec.14204 1365-294X openAccess Elevated atmospheric Co2 Carbon nutrient balance Gene-expression Climate change Posidonia-Oceanica Zostera-Marina Saccharomyces-Cerevisiae Seawater acidification Herbivore performance Calcifying organisms Carbohydrate metabolism Cymodocea nodosa Ocean acidification Protein folding Seagrasses Transcriptome article 2017 ftunivalgarve https://doi.org/10.1111/mec.14204 2022-05-30T08:48:50Z Here, we report the first use of massive-scale RNA-sequencing to explore seagrass response to CO2-driven ocean acidification (OA). Large-scale gene expression changes in the seagrass Cymodocea nodosa occurred at CO2 levels projected by the end of the century. C. nodosa transcriptome was obtained using Illumina RNA-Seq technology and de novo assembly, and differential gene expression was explored in plants exposed to short-term high CO2/low pH conditions. At high pCO(2), there was a significant increased expression of transcripts associated with photosynthesis, including light reaction functions and CO2 fixation, and also to respiratory pathways, specifically for enzymes involved in glycolysis, in the tricarboxylic acid cycle and in the energy metabolism of the mitochondrial electron transport. The upregulation of respiratory metabolism is probably supported by the increased availability of photo-synthates and increased energy demand for biosynthesis and stress-related processes under elevated CO2 and low pH. The upregulation of several chaperones resembling heat stress-induced changes in gene expression highlighted the positive role these proteins play in tolerance to intracellular acid stress in seagrasses. OA further modifies C. nodosa secondary metabolism inducing the transcription of enzymes related to biosynthesis of carbon-based secondary compounds, in particular the synthesis of polyphenols and isoprenoid compounds that have a variety of biological functions including plant defence. By demonstrating which physiological processes are most sensitive to OA, this research provides a major advance in the understanding of seagrass metabolism in the context of altered seawater chemistry from global climate change. Portuguese FCT project HighGrass [PTDC/MAR-EST/3687/2012] Article in Journal/Newspaper Ocean acidification Universidade do Algarve: Sapienta Molecular Ecology 26 16 4241 4259 |
institution |
Open Polar |
collection |
Universidade do Algarve: Sapienta |
op_collection_id |
ftunivalgarve |
language |
English |
topic |
Elevated atmospheric Co2 Carbon nutrient balance Gene-expression Climate change Posidonia-Oceanica Zostera-Marina Saccharomyces-Cerevisiae Seawater acidification Herbivore performance Calcifying organisms Carbohydrate metabolism Cymodocea nodosa Ocean acidification Protein folding Seagrasses Transcriptome |
spellingShingle |
Elevated atmospheric Co2 Carbon nutrient balance Gene-expression Climate change Posidonia-Oceanica Zostera-Marina Saccharomyces-Cerevisiae Seawater acidification Herbivore performance Calcifying organisms Carbohydrate metabolism Cymodocea nodosa Ocean acidification Protein folding Seagrasses Transcriptome Ruocco, Miriam Musacchia, Francesco Olivé, Irene Costa, Monya Barrote, Isabel Santos, Rui Sanges, Remo Procaccini, Gabriele Silva, João Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification |
topic_facet |
Elevated atmospheric Co2 Carbon nutrient balance Gene-expression Climate change Posidonia-Oceanica Zostera-Marina Saccharomyces-Cerevisiae Seawater acidification Herbivore performance Calcifying organisms Carbohydrate metabolism Cymodocea nodosa Ocean acidification Protein folding Seagrasses Transcriptome |
description |
Here, we report the first use of massive-scale RNA-sequencing to explore seagrass response to CO2-driven ocean acidification (OA). Large-scale gene expression changes in the seagrass Cymodocea nodosa occurred at CO2 levels projected by the end of the century. C. nodosa transcriptome was obtained using Illumina RNA-Seq technology and de novo assembly, and differential gene expression was explored in plants exposed to short-term high CO2/low pH conditions. At high pCO(2), there was a significant increased expression of transcripts associated with photosynthesis, including light reaction functions and CO2 fixation, and also to respiratory pathways, specifically for enzymes involved in glycolysis, in the tricarboxylic acid cycle and in the energy metabolism of the mitochondrial electron transport. The upregulation of respiratory metabolism is probably supported by the increased availability of photo-synthates and increased energy demand for biosynthesis and stress-related processes under elevated CO2 and low pH. The upregulation of several chaperones resembling heat stress-induced changes in gene expression highlighted the positive role these proteins play in tolerance to intracellular acid stress in seagrasses. OA further modifies C. nodosa secondary metabolism inducing the transcription of enzymes related to biosynthesis of carbon-based secondary compounds, in particular the synthesis of polyphenols and isoprenoid compounds that have a variety of biological functions including plant defence. By demonstrating which physiological processes are most sensitive to OA, this research provides a major advance in the understanding of seagrass metabolism in the context of altered seawater chemistry from global climate change. Portuguese FCT project HighGrass [PTDC/MAR-EST/3687/2012] |
format |
Article in Journal/Newspaper |
author |
Ruocco, Miriam Musacchia, Francesco Olivé, Irene Costa, Monya Barrote, Isabel Santos, Rui Sanges, Remo Procaccini, Gabriele Silva, João |
author_facet |
Ruocco, Miriam Musacchia, Francesco Olivé, Irene Costa, Monya Barrote, Isabel Santos, Rui Sanges, Remo Procaccini, Gabriele Silva, João |
author_sort |
Ruocco, Miriam |
title |
Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification |
title_short |
Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification |
title_full |
Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification |
title_fullStr |
Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification |
title_full_unstemmed |
Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification |
title_sort |
genomewide transcriptional reprogramming in the seagrass cymodocea nodosa under experimental ocean acidification |
publisher |
Wiley |
publishDate |
2017 |
url |
http://hdl.handle.net/10400.1/13055 https://doi.org/10.1111/mec.14204 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
info:eu-repo/grantAgreement/FCT/3599-PPCDT/130156/PT 0962-1083 http://hdl.handle.net/10400.1/13055 doi:10.1111/mec.14204 1365-294X |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1111/mec.14204 |
container_title |
Molecular Ecology |
container_volume |
26 |
container_issue |
16 |
container_start_page |
4241 |
op_container_end_page |
4259 |
_version_ |
1766156917234728960 |