Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP
In marine fish, high epithelial bicarbonate secretion by the intestine generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. In vitro studies highlight the involvement of the calciotropic hormones PTHrP (parathyroid hormone-related protein) a...
Published in: | Journal of Experimental Biology |
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Company Of Biologists Ltd
2014
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Online Access: | http://hdl.handle.net/10400.1/11706 https://doi.org/10.1242/jeb.098517 |
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ftunivalgarve:oai:sapientia.ualg.pt:10400.1/11706 2023-05-15T18:10:01+02:00 Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP Gregorio, Silvia F. Carvalho, Edison S. M. Campinho, Marco A. Power, Deborah M. Canario, Adelino V. M. Fuentes, Juan 2014-05 http://hdl.handle.net/10400.1/11706 https://doi.org/10.1242/jeb.098517 eng eng Company Of Biologists Ltd info:eu-repo/grantAgreement/FCT/3599-PPCDT/104008/PT info:eu-repo/grantAgreement/FCT/3599-PPCDT/130351/PT info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F66808%2F2009/PT 0022-0949 1477-9145 http://hdl.handle.net/10400.1/11706 doi:10.1242/jeb.098517 openAccess http://creativecommons.org/licenses/by/4.0/ CC-BY Hormone-related protein Sparus-Aurata L Soluble Adenylyl-Cyclase Salmo-Salar L Bicarbonate secretion Sea bream Parathyroid-hormone Hco3-secretion Teleost Fish Water-absorption article 2014 ftunivalgarve https://doi.org/10.1242/jeb.098517 2022-05-30T08:48:23Z In marine fish, high epithelial bicarbonate secretion by the intestine generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. In vitro studies highlight the involvement of the calciotropic hormones PTHrP (parathyroid hormone-related protein) and stanniocalcin (STC) in the regulation of epithelial bicarbonate transport. The present study tested the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo. Sea bream (Sparus aurata) juveniles received single intraperitoneal injections of piscine PTHrP(1-34), the PTH/PTHrP receptor antagonist PTHrP(7-34) or purified sea bream STC, or were passively immunized with polyclonal rabbit antisera raised against sea bream STC (STC-Ab). Endocrine effects on the expression of the basolateral sodium bicarbonate cotransporter (Slc4a4.A), the apical anion exchangers Slc26a6.A and Slc26a3.B, and the V-type proton pump beta-subunit (Atp6v1b) in the anterior intestine were evaluated. In keeping with their calciotropic nature, the hypocalcaemic factors PTHrP(7-34) and STC upregulated gene expression of all transporters. In contrast, the hypercalcaemic factor PTHrP(1-34) and STC antibodies downregulated transporters involved in the bicarbonate secretion cascade. Changes in intestine luminal precipitate contents provoked by calcaemic endocrine factors validated these results: 24 h postinjection either PTHrP(1-34) or immunization with STC-Ab reduced the carbonate precipitate content in the sea bream intestine. In contrast, the PTH/PTHrP receptor antagonist PTHrP(7-34) increased not only the precipitated fraction but also the concentration of HCO3 equivalents in the intestinal fluid. These results confirm the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo in the intestine of marine fish. Furthermore, they illustrate for the first time in fish the counteracting effect of PTHrP and STC, and reveal an unexpected contribution of ... Article in Journal/Newspaper Salmo salar Universidade do Algarve: Sapienta Journal of Experimental Biology |
institution |
Open Polar |
collection |
Universidade do Algarve: Sapienta |
op_collection_id |
ftunivalgarve |
language |
English |
topic |
Hormone-related protein Sparus-Aurata L Soluble Adenylyl-Cyclase Salmo-Salar L Bicarbonate secretion Sea bream Parathyroid-hormone Hco3-secretion Teleost Fish Water-absorption |
spellingShingle |
Hormone-related protein Sparus-Aurata L Soluble Adenylyl-Cyclase Salmo-Salar L Bicarbonate secretion Sea bream Parathyroid-hormone Hco3-secretion Teleost Fish Water-absorption Gregorio, Silvia F. Carvalho, Edison S. M. Campinho, Marco A. Power, Deborah M. Canario, Adelino V. M. Fuentes, Juan Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP |
topic_facet |
Hormone-related protein Sparus-Aurata L Soluble Adenylyl-Cyclase Salmo-Salar L Bicarbonate secretion Sea bream Parathyroid-hormone Hco3-secretion Teleost Fish Water-absorption |
description |
In marine fish, high epithelial bicarbonate secretion by the intestine generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. In vitro studies highlight the involvement of the calciotropic hormones PTHrP (parathyroid hormone-related protein) and stanniocalcin (STC) in the regulation of epithelial bicarbonate transport. The present study tested the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo. Sea bream (Sparus aurata) juveniles received single intraperitoneal injections of piscine PTHrP(1-34), the PTH/PTHrP receptor antagonist PTHrP(7-34) or purified sea bream STC, or were passively immunized with polyclonal rabbit antisera raised against sea bream STC (STC-Ab). Endocrine effects on the expression of the basolateral sodium bicarbonate cotransporter (Slc4a4.A), the apical anion exchangers Slc26a6.A and Slc26a3.B, and the V-type proton pump beta-subunit (Atp6v1b) in the anterior intestine were evaluated. In keeping with their calciotropic nature, the hypocalcaemic factors PTHrP(7-34) and STC upregulated gene expression of all transporters. In contrast, the hypercalcaemic factor PTHrP(1-34) and STC antibodies downregulated transporters involved in the bicarbonate secretion cascade. Changes in intestine luminal precipitate contents provoked by calcaemic endocrine factors validated these results: 24 h postinjection either PTHrP(1-34) or immunization with STC-Ab reduced the carbonate precipitate content in the sea bream intestine. In contrast, the PTH/PTHrP receptor antagonist PTHrP(7-34) increased not only the precipitated fraction but also the concentration of HCO3 equivalents in the intestinal fluid. These results confirm the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo in the intestine of marine fish. Furthermore, they illustrate for the first time in fish the counteracting effect of PTHrP and STC, and reveal an unexpected contribution of ... |
format |
Article in Journal/Newspaper |
author |
Gregorio, Silvia F. Carvalho, Edison S. M. Campinho, Marco A. Power, Deborah M. Canario, Adelino V. M. Fuentes, Juan |
author_facet |
Gregorio, Silvia F. Carvalho, Edison S. M. Campinho, Marco A. Power, Deborah M. Canario, Adelino V. M. Fuentes, Juan |
author_sort |
Gregorio, Silvia F. |
title |
Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP |
title_short |
Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP |
title_full |
Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP |
title_fullStr |
Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP |
title_full_unstemmed |
Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP |
title_sort |
endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and pthrp |
publisher |
Company Of Biologists Ltd |
publishDate |
2014 |
url |
http://hdl.handle.net/10400.1/11706 https://doi.org/10.1242/jeb.098517 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
info:eu-repo/grantAgreement/FCT/3599-PPCDT/104008/PT info:eu-repo/grantAgreement/FCT/3599-PPCDT/130351/PT info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F66808%2F2009/PT 0022-0949 1477-9145 http://hdl.handle.net/10400.1/11706 doi:10.1242/jeb.098517 |
op_rights |
openAccess http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1242/jeb.098517 |
container_title |
Journal of Experimental Biology |
_version_ |
1766182735152414720 |