Vasotocin and isotocin regulate aquaporin 1 function in the sea bream
Aquaporins (AQPs) are specific transmembrane water channels with an important function in water homeostasis. In terrestrial vertebrates, AQP2 function is regulated by vasopressin (AVP) to accomplish key functions in osmoregulation. The endocrine control of aquaporin function in teleosts remains litt...
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Company Of Biologists Ltd
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Online Access: | http://hdl.handle.net/10400.1/11637 https://doi.org/10.1242/jeb.114546 |
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ftunivalgarve:oai:sapientia.ualg.pt:10400.1/11637 2023-05-15T13:28:08+02:00 Vasotocin and isotocin regulate aquaporin 1 function in the sea bream Martos-Sitcha, Juan Antonio Campinho, Marco António Miguel Mancera, Juan Martinez-Rodriguez, Gonzalo Fuentes, Juan 2015-03 http://hdl.handle.net/10400.1/11637 https://doi.org/10.1242/jeb.114546 eng eng Company Of Biologists Ltd FPU AP2008-01194 info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F66808%2F2009/PT AGL2010-14876 info:eu-repo/grantAgreement/FCT/3599-PPCDT/130351/PT 0022-0949 1477-9145 http://hdl.handle.net/10400.1/11637 doi:10.1242/jeb.114546 openAccess Collecting Duct Cells Eel Anguilla-Anguilla Flounder Platichthys-Flesus Water Channel Arginine-Vasotocin Sparus-Aurata Seawater Acclimation Molecular-Cloning Xenopus Oocytes Messenger-Rna article 2015 ftunivalgarve https://doi.org/10.1242/jeb.114546 2022-05-30T08:48:21Z Aquaporins (AQPs) are specific transmembrane water channels with an important function in water homeostasis. In terrestrial vertebrates, AQP2 function is regulated by vasopressin (AVP) to accomplish key functions in osmoregulation. The endocrine control of aquaporin function in teleosts remains little studied. Therefore, in this study we investigated the regulatory role of vasotocin (AVTR) and isotocin (ITR) receptors in Aqp1 paralog gene function in the teleost gilthead sea bream (Sparus aurata). The complete coding regions of Aqp1a, Aqp1b, AVTR V1a2-type, AVTR V2-type and ITR from sea bream were isolated. A Xenopus oocyte-swelling assay was used to functionally characterize AQP1 function and regulation by AVT and IT through their cognate receptors. Microinjection of oocytes with Aqp1b mRNA revealed regulation of water transport via PKA (IBMX+forskolin sensitive), whereas Aqp1a mRNA injection had the same effect via PKC signaling (PDBU sensitive). In the absence of expressed receptors, AVT and IT (10(-8) mol l(-1)) were unable to modify AQP1 function. AVT regulated AQP1a and AQP1b function only when the AVTR V2-type was co-expressed. IT regulated AQP1a function, but not AQP1b, only when ITR was present. Considering that Aqp1a and Aqp1b gene expression in the sea bream intestine is highly salinity dependent in vivo, our results in ovo demonstrate a regulatory role for AVT and IT in AQP1 function in the sea bream in the processing of intestinal fluid to achieve osmoregulation. Ministry of Education (Spain) [FPU AP2008-01194]; Portuguese National Science Foundation [SFRH/BPD/66808/2009]; Ministry of Science and Education, Spain [AGL2010-14876]; National Science Foundation (FCT, Portugal) [PTDC/MAR-BIO/3811/2012] Article in Journal/Newspaper Anguilla anguilla Universidade do Algarve: Sapienta Journal of Experimental Biology 218 5 684 693 |
institution |
Open Polar |
collection |
Universidade do Algarve: Sapienta |
op_collection_id |
ftunivalgarve |
language |
English |
topic |
Collecting Duct Cells Eel Anguilla-Anguilla Flounder Platichthys-Flesus Water Channel Arginine-Vasotocin Sparus-Aurata Seawater Acclimation Molecular-Cloning Xenopus Oocytes Messenger-Rna |
spellingShingle |
Collecting Duct Cells Eel Anguilla-Anguilla Flounder Platichthys-Flesus Water Channel Arginine-Vasotocin Sparus-Aurata Seawater Acclimation Molecular-Cloning Xenopus Oocytes Messenger-Rna Martos-Sitcha, Juan Antonio Campinho, Marco António Miguel Mancera, Juan Martinez-Rodriguez, Gonzalo Fuentes, Juan Vasotocin and isotocin regulate aquaporin 1 function in the sea bream |
topic_facet |
Collecting Duct Cells Eel Anguilla-Anguilla Flounder Platichthys-Flesus Water Channel Arginine-Vasotocin Sparus-Aurata Seawater Acclimation Molecular-Cloning Xenopus Oocytes Messenger-Rna |
description |
Aquaporins (AQPs) are specific transmembrane water channels with an important function in water homeostasis. In terrestrial vertebrates, AQP2 function is regulated by vasopressin (AVP) to accomplish key functions in osmoregulation. The endocrine control of aquaporin function in teleosts remains little studied. Therefore, in this study we investigated the regulatory role of vasotocin (AVTR) and isotocin (ITR) receptors in Aqp1 paralog gene function in the teleost gilthead sea bream (Sparus aurata). The complete coding regions of Aqp1a, Aqp1b, AVTR V1a2-type, AVTR V2-type and ITR from sea bream were isolated. A Xenopus oocyte-swelling assay was used to functionally characterize AQP1 function and regulation by AVT and IT through their cognate receptors. Microinjection of oocytes with Aqp1b mRNA revealed regulation of water transport via PKA (IBMX+forskolin sensitive), whereas Aqp1a mRNA injection had the same effect via PKC signaling (PDBU sensitive). In the absence of expressed receptors, AVT and IT (10(-8) mol l(-1)) were unable to modify AQP1 function. AVT regulated AQP1a and AQP1b function only when the AVTR V2-type was co-expressed. IT regulated AQP1a function, but not AQP1b, only when ITR was present. Considering that Aqp1a and Aqp1b gene expression in the sea bream intestine is highly salinity dependent in vivo, our results in ovo demonstrate a regulatory role for AVT and IT in AQP1 function in the sea bream in the processing of intestinal fluid to achieve osmoregulation. Ministry of Education (Spain) [FPU AP2008-01194]; Portuguese National Science Foundation [SFRH/BPD/66808/2009]; Ministry of Science and Education, Spain [AGL2010-14876]; National Science Foundation (FCT, Portugal) [PTDC/MAR-BIO/3811/2012] |
format |
Article in Journal/Newspaper |
author |
Martos-Sitcha, Juan Antonio Campinho, Marco António Miguel Mancera, Juan Martinez-Rodriguez, Gonzalo Fuentes, Juan |
author_facet |
Martos-Sitcha, Juan Antonio Campinho, Marco António Miguel Mancera, Juan Martinez-Rodriguez, Gonzalo Fuentes, Juan |
author_sort |
Martos-Sitcha, Juan Antonio |
title |
Vasotocin and isotocin regulate aquaporin 1 function in the sea bream |
title_short |
Vasotocin and isotocin regulate aquaporin 1 function in the sea bream |
title_full |
Vasotocin and isotocin regulate aquaporin 1 function in the sea bream |
title_fullStr |
Vasotocin and isotocin regulate aquaporin 1 function in the sea bream |
title_full_unstemmed |
Vasotocin and isotocin regulate aquaporin 1 function in the sea bream |
title_sort |
vasotocin and isotocin regulate aquaporin 1 function in the sea bream |
publisher |
Company Of Biologists Ltd |
publishDate |
2015 |
url |
http://hdl.handle.net/10400.1/11637 https://doi.org/10.1242/jeb.114546 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
FPU AP2008-01194 info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F66808%2F2009/PT AGL2010-14876 info:eu-repo/grantAgreement/FCT/3599-PPCDT/130351/PT 0022-0949 1477-9145 http://hdl.handle.net/10400.1/11637 doi:10.1242/jeb.114546 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1242/jeb.114546 |
container_title |
Journal of Experimental Biology |
container_volume |
218 |
container_issue |
5 |
container_start_page |
684 |
op_container_end_page |
693 |
_version_ |
1766402400861552640 |