Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis
Background: The identification of DNA methyltransferases (Dnmt) expression patterns during development and their regulation is important to understand the epigenetic mechanisms that modulate larval plasticity in marine fish. In this study, dnmt1 and dnmt3 paralogs were identified in the flatfish Sol...
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Biomed Central Ltd
2017
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ftunivalgarve:oai:sapientia.ualg.pt:10400.1/11609 2023-08-27T04:11:45+02:00 Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis Firmino, Joana Carballo, Carlos Armesto, Paula Campinho, Marco António Power, Deborah M. Manchado, Manuel 2017 http://hdl.handle.net/10400.1/11609 https://doi.org/10.1186/s12861-017-0154-0 eng eng Biomed Central Ltd info:eu-repo/grantAgreement/EC/H2020/691102/EU 1471-213X http://hdl.handle.net/10400.1/11609 doi:10.1186/s12861-017-0154-0 openAccess Muscle-Fiber Recruitment Salmon Salmo-Salar Gene-Expression Teleost Fish Embryonic-Development Molecular Characterization Epigenetic Regulation Dicentrarchus-Labrax Thermal Plasticity Somatic Growth article 2017 ftunivalgarve https://doi.org/10.1186/s12861-017-0154-0 2023-08-09T00:03:58Z Background: The identification of DNA methyltransferases (Dnmt) expression patterns during development and their regulation is important to understand the epigenetic mechanisms that modulate larval plasticity in marine fish. In this study, dnmt1 and dnmt3 paralogs were identified in the flatfish Solea senegalensis and expression patterns in early developmental stages and juveniles were determined. Additionally, the regulation of Dnmt transcription by a specific inhibitor (5-aza-2 '-deoxycytidine) and temperature was evaluated. Results: Five paralog genes of dnmt3, namely dnmt3aa, dnmt3ab, dnmt3ba, dnmt3bb. 1 and dnmt3bb. 2 and one gene for dnmt1 were identified. Phylogenetic analysis revealed that the dnmt gene family was highly conserved in teleosts and three fish-specific genes, dnmt3aa, dnmt3ba and dnmt3bb. 2 have evolved. The spatio-temporal expression patterns of four dnmts (dnmt1, dnmt3aa, dnmt3ab and dnmt3bb. 1) were different in early larval stages although all of them reduced expression with the age and were detected in neural organs and dnmt3aa appeared specific to somites. In juveniles, the four dnmt genes were expressed in brain and hematopoietic tissues such as kidney, spleen and gills. Treatment of sole embryos with 5-aza-2 '-deoxycytidine down-regulated dntm1 and up-regulated dntm3aa. Moreover, in lecithotrophic larval stages, dnmt3aa and dnmt3ab were temperature sensitive and their expression was higher in larvae incubated at 16 degrees C relative to 20 degrees C. Conclusion: Five dnmt3 and one dnmt1 paralog were identified in sole and their distinct developmental and tissue-specific expression patterns indicate that they may have different roles during development. The inhibitor 5-aza-2 '-deoxycytidine modified the transcript abundance of dntm1 and dntm3aa in embryos, which suggests that a regulatory feedback mechanism exists for these genes. The impact of thermal regime on expression levels of dnmt3aa and dnmt3ab in lecithotrophic larval stages suggests that these paralogs might be involved in ... Article in Journal/Newspaper Salmo salar Universidade do Algarve: Sapienta BMC Developmental Biology 17 1 |
institution |
Open Polar |
collection |
Universidade do Algarve: Sapienta |
op_collection_id |
ftunivalgarve |
language |
English |
topic |
Muscle-Fiber Recruitment Salmon Salmo-Salar Gene-Expression Teleost Fish Embryonic-Development Molecular Characterization Epigenetic Regulation Dicentrarchus-Labrax Thermal Plasticity Somatic Growth |
spellingShingle |
Muscle-Fiber Recruitment Salmon Salmo-Salar Gene-Expression Teleost Fish Embryonic-Development Molecular Characterization Epigenetic Regulation Dicentrarchus-Labrax Thermal Plasticity Somatic Growth Firmino, Joana Carballo, Carlos Armesto, Paula Campinho, Marco António Power, Deborah M. Manchado, Manuel Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis |
topic_facet |
Muscle-Fiber Recruitment Salmon Salmo-Salar Gene-Expression Teleost Fish Embryonic-Development Molecular Characterization Epigenetic Regulation Dicentrarchus-Labrax Thermal Plasticity Somatic Growth |
description |
Background: The identification of DNA methyltransferases (Dnmt) expression patterns during development and their regulation is important to understand the epigenetic mechanisms that modulate larval plasticity in marine fish. In this study, dnmt1 and dnmt3 paralogs were identified in the flatfish Solea senegalensis and expression patterns in early developmental stages and juveniles were determined. Additionally, the regulation of Dnmt transcription by a specific inhibitor (5-aza-2 '-deoxycytidine) and temperature was evaluated. Results: Five paralog genes of dnmt3, namely dnmt3aa, dnmt3ab, dnmt3ba, dnmt3bb. 1 and dnmt3bb. 2 and one gene for dnmt1 were identified. Phylogenetic analysis revealed that the dnmt gene family was highly conserved in teleosts and three fish-specific genes, dnmt3aa, dnmt3ba and dnmt3bb. 2 have evolved. The spatio-temporal expression patterns of four dnmts (dnmt1, dnmt3aa, dnmt3ab and dnmt3bb. 1) were different in early larval stages although all of them reduced expression with the age and were detected in neural organs and dnmt3aa appeared specific to somites. In juveniles, the four dnmt genes were expressed in brain and hematopoietic tissues such as kidney, spleen and gills. Treatment of sole embryos with 5-aza-2 '-deoxycytidine down-regulated dntm1 and up-regulated dntm3aa. Moreover, in lecithotrophic larval stages, dnmt3aa and dnmt3ab were temperature sensitive and their expression was higher in larvae incubated at 16 degrees C relative to 20 degrees C. Conclusion: Five dnmt3 and one dnmt1 paralog were identified in sole and their distinct developmental and tissue-specific expression patterns indicate that they may have different roles during development. The inhibitor 5-aza-2 '-deoxycytidine modified the transcript abundance of dntm1 and dntm3aa in embryos, which suggests that a regulatory feedback mechanism exists for these genes. The impact of thermal regime on expression levels of dnmt3aa and dnmt3ab in lecithotrophic larval stages suggests that these paralogs might be involved in ... |
format |
Article in Journal/Newspaper |
author |
Firmino, Joana Carballo, Carlos Armesto, Paula Campinho, Marco António Power, Deborah M. Manchado, Manuel |
author_facet |
Firmino, Joana Carballo, Carlos Armesto, Paula Campinho, Marco António Power, Deborah M. Manchado, Manuel |
author_sort |
Firmino, Joana |
title |
Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis |
title_short |
Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis |
title_full |
Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis |
title_fullStr |
Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis |
title_full_unstemmed |
Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis |
title_sort |
phylogeny, expression patterns and regulation of dna methyltransferases in early development of the flatfish, solea senegalensis |
publisher |
Biomed Central Ltd |
publishDate |
2017 |
url |
http://hdl.handle.net/10400.1/11609 https://doi.org/10.1186/s12861-017-0154-0 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
info:eu-repo/grantAgreement/EC/H2020/691102/EU 1471-213X http://hdl.handle.net/10400.1/11609 doi:10.1186/s12861-017-0154-0 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1186/s12861-017-0154-0 |
container_title |
BMC Developmental Biology |
container_volume |
17 |
container_issue |
1 |
_version_ |
1775354808820039680 |