Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE)
The pressurized liquid extraction (PLE) technique was used to obtain protein extracts with antioxidant capacity from salmon muscle remains, heads, viscera, skin, and tailfins. A protein recovery percentage ≈28% was obtained for all samples except for viscera, which was ≈92%. These values represented...
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Language: | English |
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MDPI AG
2023
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Online Access: | https://hdl.handle.net/10550/91950 https://doi.org/10.3390/md19060323 |
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ftunivalencia:oai:roderic.uv.es:10550/91950 2024-02-04T10:04:16+01:00 Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) de la Fuente Miguel, Beatriz Pallarés Barrachina, Noelia Berrada Ramdani, Houda Barba Orellana, Francisco José 2023-12-27T10:23:07Z application/pdf https://hdl.handle.net/10550/91950 https://doi.org/10.3390/md19060323 eng eng MDPI AG Marine Drugs, 2021, vol. 19, num. 6, p. Art. 323 de la Fuente, B., Pallarés, N., Berrada, H., & Barba, F. J. (2021). Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE). En Marine Drugs (Vol. 19, Issue 6, p. 323). MDPI AG. https://doi.org/10.3390/md19060323 1660-3397 https://hdl.handle.net/10550/91950 doi:10.3390/md19060323 150057 open access de la Fuente Miguel, Beatriz Pallarés Barrachina, Noelia Berrada Ramdani, Houda Barba Orellana, Francisco José 2021 Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) Marine Drugs 19 6 Art. 323 peix pèptids farmacologia aliments deshidratació UNESCO::CIENCIAS MÉDICAS journal article NA 2023 ftunivalencia https://doi.org/10.3390/md19060323 2024-01-10T00:07:42Z The pressurized liquid extraction (PLE) technique was used to obtain protein extracts with antioxidant capacity from salmon muscle remains, heads, viscera, skin, and tailfins. A protein recovery percentage ≈28% was obtained for all samples except for viscera, which was ≈92%. These values represented an increase of 1.5-4.8-fold compared to stirring extraction (control). Different SDS-PAGE profiles in control and PLE extracts revealed that extraction conditions affected the protein molecular weight distribution of the obtained extracts. Both TEAC (Trolox equivalent antioxidant capacity) and ORAC (oxygen radical antioxidant capacity) assays showed an outstanding antioxidant activity for viscera PLE extract. Through liquid chromatography coupled with electrospray ionization triple time-of-flight (nanoESI qQTOF) mass spectrometry, 137 and 67 peptides were identified in control and PLE extracts from salmon viscera, respectively None of these peptides was found among the antioxidant peptides inputted in the BIOPEP-UMP database. However, bioinformatics analysis showed several antioxidant small peptides encrypted in amino acid sequences of viscera extracts, especially GPP (glycine-proline-proline) and GAA (glycine-alanine-alanine) for PLE extracts. Further research on the relationship between antioxidant activity and specific peptides from salmon viscera PLE extracts is required. In addition, the salmon side streams studied presented non-toxic levels of As, Hg, Cd, and Pb, as well as the absence of mycotoxins or related metabolites. Overall, these results confirm the feasible use of farmed salmon processing side streams as alternative sources of protein and bioactive compounds for human consumption. Article in Journal/Newspaper Salmo salar Universitat de València: Roderic - Repositorio de contenido libre Marine Drugs 19 6 323 |
institution |
Open Polar |
collection |
Universitat de València: Roderic - Repositorio de contenido libre |
op_collection_id |
ftunivalencia |
language |
English |
topic |
peix pèptids farmacologia aliments deshidratació UNESCO::CIENCIAS MÉDICAS |
spellingShingle |
peix pèptids farmacologia aliments deshidratació UNESCO::CIENCIAS MÉDICAS de la Fuente Miguel, Beatriz Pallarés Barrachina, Noelia Berrada Ramdani, Houda Barba Orellana, Francisco José Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
topic_facet |
peix pèptids farmacologia aliments deshidratació UNESCO::CIENCIAS MÉDICAS |
description |
The pressurized liquid extraction (PLE) technique was used to obtain protein extracts with antioxidant capacity from salmon muscle remains, heads, viscera, skin, and tailfins. A protein recovery percentage ≈28% was obtained for all samples except for viscera, which was ≈92%. These values represented an increase of 1.5-4.8-fold compared to stirring extraction (control). Different SDS-PAGE profiles in control and PLE extracts revealed that extraction conditions affected the protein molecular weight distribution of the obtained extracts. Both TEAC (Trolox equivalent antioxidant capacity) and ORAC (oxygen radical antioxidant capacity) assays showed an outstanding antioxidant activity for viscera PLE extract. Through liquid chromatography coupled with electrospray ionization triple time-of-flight (nanoESI qQTOF) mass spectrometry, 137 and 67 peptides were identified in control and PLE extracts from salmon viscera, respectively None of these peptides was found among the antioxidant peptides inputted in the BIOPEP-UMP database. However, bioinformatics analysis showed several antioxidant small peptides encrypted in amino acid sequences of viscera extracts, especially GPP (glycine-proline-proline) and GAA (glycine-alanine-alanine) for PLE extracts. Further research on the relationship between antioxidant activity and specific peptides from salmon viscera PLE extracts is required. In addition, the salmon side streams studied presented non-toxic levels of As, Hg, Cd, and Pb, as well as the absence of mycotoxins or related metabolites. Overall, these results confirm the feasible use of farmed salmon processing side streams as alternative sources of protein and bioactive compounds for human consumption. |
format |
Article in Journal/Newspaper |
author |
de la Fuente Miguel, Beatriz Pallarés Barrachina, Noelia Berrada Ramdani, Houda Barba Orellana, Francisco José |
author_facet |
de la Fuente Miguel, Beatriz Pallarés Barrachina, Noelia Berrada Ramdani, Houda Barba Orellana, Francisco José |
author_sort |
de la Fuente Miguel, Beatriz |
title |
Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_short |
Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_full |
Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_fullStr |
Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_full_unstemmed |
Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_sort |
salmon (salmo salar) side streams as a bioresource to obtain potential antioxidant peptides after applying pressurized liquid extraction (ple) |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://hdl.handle.net/10550/91950 https://doi.org/10.3390/md19060323 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
de la Fuente Miguel, Beatriz Pallarés Barrachina, Noelia Berrada Ramdani, Houda Barba Orellana, Francisco José 2021 Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) Marine Drugs 19 6 Art. 323 |
op_relation |
Marine Drugs, 2021, vol. 19, num. 6, p. Art. 323 de la Fuente, B., Pallarés, N., Berrada, H., & Barba, F. J. (2021). Salmon (Salmo salar) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE). En Marine Drugs (Vol. 19, Issue 6, p. 323). MDPI AG. https://doi.org/10.3390/md19060323 1660-3397 https://hdl.handle.net/10550/91950 doi:10.3390/md19060323 150057 |
op_rights |
open access |
op_doi |
https://doi.org/10.3390/md19060323 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
6 |
container_start_page |
323 |
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1789972414080221184 |