Vibrio vulnificus: from water to host
Vibrio vulnificus is an aquatic pathogen autochthonous from temperate, tropical and subtropical ecosystems where it lives either as a sessile cell, forming biofilms or as a free-swimming cell. From these locations, the pathogen can occasionally infect humans and fish causing a disease named vibriosi...
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ftunivalencia:oai:roderic.uv.es:10550/56878 2023-06-11T04:03:47+02:00 Vibrio vulnificus: from water to host Carda Diéguez, Miguel Amaro González, Carmen Rodríguez Valera, Francisco Eduardo Departament de Microbiologia i Ecologia 2017 230 p. http://hdl.handle.net/10550/56878 en eng http://hdl.handle.net/10550/56878 microbiología microbiology metagenomics Vibrio Vibrio vulnificus Anguilla anguilla anguila European eel transposon insertion sequencing microbiota host-associated microbiota UNESCO::CIENCIAS DE LA VIDA doctoral thesis 2017 ftunivalencia 2023-04-18T23:40:35Z Vibrio vulnificus is an aquatic pathogen autochthonous from temperate, tropical and subtropical ecosystems where it lives either as a sessile cell, forming biofilms or as a free-swimming cell. From these locations, the pathogen can occasionally infect humans and fish causing a disease named vibriosis. The most severe form of human and fish vibriosis is associated with the pathogen’s ability to spread from the infection site to the bloodstream and multiply, process known as invasion. Before invasion, the pathogen has to colonize the mucosal host surface, process that involves not only bacterial attachment/adhesion but also resistance to mucosal immunity, commensal microbiota (competitors) and bacterial predators (mainly amoeba and phages). Recently, Amaro and cols. obtained evidence that supports that mucin, main protein in mucus, can activate horizontal gene transference in V. vulnificus, which could lead to the emergence of new virulent clones in natural mucosal environments. The objective of this thesis was to study the colonization and invasion processes under the global perspective that allow the “omic” technologies. In the first chapter, we focused our attention on a selected host for the pathogen in the aquatic environment, the eel, and analyzed its microbiome by using metagenomics. We describe for the first time the microbiome of the skin mucus of wild- and farmed-eels and compared it with that of the water. We discovered that mucus concentrates, selectively, bacteria present in water and identified the genes involved in a successful colonization process, most of which could be considered virulence genes. Then, we developed a protocol to identify MGE and prophages in the metagenomes and described a series of putative ICEs, pathogenicity islands and prophages some of which contained virulence and antibiotic resistance genes. Finally, we were able to describe multiple lytic phages, which could be considered as a part of the mucosal immunity. The second chapter of this thesis is focused on the invasion, and, ... Doctoral or Postdoctoral Thesis Anguilla anguilla Universitat de València: Roderic - Repositorio de contenido libre |
institution |
Open Polar |
collection |
Universitat de València: Roderic - Repositorio de contenido libre |
op_collection_id |
ftunivalencia |
language |
English |
topic |
microbiología microbiology metagenomics Vibrio Vibrio vulnificus Anguilla anguilla anguila European eel transposon insertion sequencing microbiota host-associated microbiota UNESCO::CIENCIAS DE LA VIDA |
spellingShingle |
microbiología microbiology metagenomics Vibrio Vibrio vulnificus Anguilla anguilla anguila European eel transposon insertion sequencing microbiota host-associated microbiota UNESCO::CIENCIAS DE LA VIDA Carda Diéguez, Miguel Vibrio vulnificus: from water to host |
topic_facet |
microbiología microbiology metagenomics Vibrio Vibrio vulnificus Anguilla anguilla anguila European eel transposon insertion sequencing microbiota host-associated microbiota UNESCO::CIENCIAS DE LA VIDA |
description |
Vibrio vulnificus is an aquatic pathogen autochthonous from temperate, tropical and subtropical ecosystems where it lives either as a sessile cell, forming biofilms or as a free-swimming cell. From these locations, the pathogen can occasionally infect humans and fish causing a disease named vibriosis. The most severe form of human and fish vibriosis is associated with the pathogen’s ability to spread from the infection site to the bloodstream and multiply, process known as invasion. Before invasion, the pathogen has to colonize the mucosal host surface, process that involves not only bacterial attachment/adhesion but also resistance to mucosal immunity, commensal microbiota (competitors) and bacterial predators (mainly amoeba and phages). Recently, Amaro and cols. obtained evidence that supports that mucin, main protein in mucus, can activate horizontal gene transference in V. vulnificus, which could lead to the emergence of new virulent clones in natural mucosal environments. The objective of this thesis was to study the colonization and invasion processes under the global perspective that allow the “omic” technologies. In the first chapter, we focused our attention on a selected host for the pathogen in the aquatic environment, the eel, and analyzed its microbiome by using metagenomics. We describe for the first time the microbiome of the skin mucus of wild- and farmed-eels and compared it with that of the water. We discovered that mucus concentrates, selectively, bacteria present in water and identified the genes involved in a successful colonization process, most of which could be considered virulence genes. Then, we developed a protocol to identify MGE and prophages in the metagenomes and described a series of putative ICEs, pathogenicity islands and prophages some of which contained virulence and antibiotic resistance genes. Finally, we were able to describe multiple lytic phages, which could be considered as a part of the mucosal immunity. The second chapter of this thesis is focused on the invasion, and, ... |
author2 |
Amaro González, Carmen Rodríguez Valera, Francisco Eduardo Departament de Microbiologia i Ecologia |
format |
Doctoral or Postdoctoral Thesis |
author |
Carda Diéguez, Miguel |
author_facet |
Carda Diéguez, Miguel |
author_sort |
Carda Diéguez, Miguel |
title |
Vibrio vulnificus: from water to host |
title_short |
Vibrio vulnificus: from water to host |
title_full |
Vibrio vulnificus: from water to host |
title_fullStr |
Vibrio vulnificus: from water to host |
title_full_unstemmed |
Vibrio vulnificus: from water to host |
title_sort |
vibrio vulnificus: from water to host |
publishDate |
2017 |
url |
http://hdl.handle.net/10550/56878 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
http://hdl.handle.net/10550/56878 |
_version_ |
1768383376886071296 |