δ13C and δ15N Signatures in Muscle and Fin Tissues: Nonlethal Sampling Methods for Stable Isotope Analysis of Salmonid
Stable isotope analysis has emerged as an important tool in aquatic ecology. For fish, dorsal muscle from sacrificed individuals has traditionally been used in stable isotope studies; however, there are many instances when lethal sampling is undesirable. We evaluated the feasibility of using adipose...
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ftunivalberta:oai:era.library.ualberta.ca:d6c2b0cc-7476-4982-951d-ee66a0190106 2023-05-15T15:32:44+02:00 δ13C and δ15N Signatures in Muscle and Fin Tissues: Nonlethal Sampling Methods for Stable Isotope Analysis of Salmonid Paszkowski, C.A. Hanisch, J.R. Tonn, W.M. Scrimgeour, G.J. 2010 https://era.library.ualberta.ca/items/d6c2b0cc-7476-4982-951d-ee66a0190106 https://doi.org/10.7939/R3BV7B61J English eng https://era.library.ualberta.ca/items/d6c2b0cc-7476-4982-951d-ee66a0190106 doi:10.7939/R3BV7B61J © Copyright by the American Fisheries Society 2009 Adipose fin Salar Atlantic salmon Lake food webs Nitrogen isotope Fresh-water Rainbow-trout Carbon isotopes Fish-tissues Ratios Article (Published) 2010 ftunivalberta https://doi.org/10.7939/R3BV7B61J 2022-08-22T20:14:10Z Stable isotope analysis has emerged as an important tool in aquatic ecology. For fish, dorsal muscle from sacrificed individuals has traditionally been used in stable isotope studies; however, there are many instances when lethal sampling is undesirable. We evaluated the feasibility of using adipose and caudal fin clips as alternatives to muscle in stable isotope studies for five species of salmonids. Because fish size and water temperature can affect stable isotope ratios, we also determined whether fish length and sampling date affected the difference in isotope signatures between fins and muscle. Biopsied muscle plugs and fin clips were collected from rainbow trout Oncorhynchus mykiss, brook trout Salvelinus fontinalis, and lake trout S. namaycush as well as lake whitefish Coregonus clupeaformis and pygmy whitefish Prosopium coulterii and analyzed for stable isotopes of carbon and nitrogen. The isotope signatures of both adipose and caudal fins were significantly correlated (0.33 , R2 , 0.97) with those of dorsal muscle from the five salmonid species studied, and in some cases the signatures of fins did not differ from those of muscle. Fish length and sampling date occasionally had a small effect (0.042 , R2 , 0.49) on the relationship between the isotope signatures of fin and muscle. Although muscle biopsy provides a viable, nonlethal method of collecting muscle tissue from suitably sized fish, the strong relationships between the isotope signatures of fin and muscle demonstrate that fin clips should be considered good surrogates for muscle in stable isotope studies of salmonids. Other/Unknown Material Atlantic salmon Pygmy whitefish University of Alberta: Era - Education and Research Archive |
institution |
Open Polar |
collection |
University of Alberta: Era - Education and Research Archive |
op_collection_id |
ftunivalberta |
language |
English |
topic |
Adipose fin Salar Atlantic salmon Lake food webs Nitrogen isotope Fresh-water Rainbow-trout Carbon isotopes Fish-tissues Ratios |
spellingShingle |
Adipose fin Salar Atlantic salmon Lake food webs Nitrogen isotope Fresh-water Rainbow-trout Carbon isotopes Fish-tissues Ratios Paszkowski, C.A. Hanisch, J.R. Tonn, W.M. Scrimgeour, G.J. δ13C and δ15N Signatures in Muscle and Fin Tissues: Nonlethal Sampling Methods for Stable Isotope Analysis of Salmonid |
topic_facet |
Adipose fin Salar Atlantic salmon Lake food webs Nitrogen isotope Fresh-water Rainbow-trout Carbon isotopes Fish-tissues Ratios |
description |
Stable isotope analysis has emerged as an important tool in aquatic ecology. For fish, dorsal muscle from sacrificed individuals has traditionally been used in stable isotope studies; however, there are many instances when lethal sampling is undesirable. We evaluated the feasibility of using adipose and caudal fin clips as alternatives to muscle in stable isotope studies for five species of salmonids. Because fish size and water temperature can affect stable isotope ratios, we also determined whether fish length and sampling date affected the difference in isotope signatures between fins and muscle. Biopsied muscle plugs and fin clips were collected from rainbow trout Oncorhynchus mykiss, brook trout Salvelinus fontinalis, and lake trout S. namaycush as well as lake whitefish Coregonus clupeaformis and pygmy whitefish Prosopium coulterii and analyzed for stable isotopes of carbon and nitrogen. The isotope signatures of both adipose and caudal fins were significantly correlated (0.33 , R2 , 0.97) with those of dorsal muscle from the five salmonid species studied, and in some cases the signatures of fins did not differ from those of muscle. Fish length and sampling date occasionally had a small effect (0.042 , R2 , 0.49) on the relationship between the isotope signatures of fin and muscle. Although muscle biopsy provides a viable, nonlethal method of collecting muscle tissue from suitably sized fish, the strong relationships between the isotope signatures of fin and muscle demonstrate that fin clips should be considered good surrogates for muscle in stable isotope studies of salmonids. |
format |
Other/Unknown Material |
author |
Paszkowski, C.A. Hanisch, J.R. Tonn, W.M. Scrimgeour, G.J. |
author_facet |
Paszkowski, C.A. Hanisch, J.R. Tonn, W.M. Scrimgeour, G.J. |
author_sort |
Paszkowski, C.A. |
title |
δ13C and δ15N Signatures in Muscle and Fin Tissues: Nonlethal Sampling Methods for Stable Isotope Analysis of Salmonid |
title_short |
δ13C and δ15N Signatures in Muscle and Fin Tissues: Nonlethal Sampling Methods for Stable Isotope Analysis of Salmonid |
title_full |
δ13C and δ15N Signatures in Muscle and Fin Tissues: Nonlethal Sampling Methods for Stable Isotope Analysis of Salmonid |
title_fullStr |
δ13C and δ15N Signatures in Muscle and Fin Tissues: Nonlethal Sampling Methods for Stable Isotope Analysis of Salmonid |
title_full_unstemmed |
δ13C and δ15N Signatures in Muscle and Fin Tissues: Nonlethal Sampling Methods for Stable Isotope Analysis of Salmonid |
title_sort |
δ13c and δ15n signatures in muscle and fin tissues: nonlethal sampling methods for stable isotope analysis of salmonid |
publishDate |
2010 |
url |
https://era.library.ualberta.ca/items/d6c2b0cc-7476-4982-951d-ee66a0190106 https://doi.org/10.7939/R3BV7B61J |
genre |
Atlantic salmon Pygmy whitefish |
genre_facet |
Atlantic salmon Pygmy whitefish |
op_relation |
https://era.library.ualberta.ca/items/d6c2b0cc-7476-4982-951d-ee66a0190106 doi:10.7939/R3BV7B61J |
op_rights |
© Copyright by the American Fisheries Society 2009 |
op_doi |
https://doi.org/10.7939/R3BV7B61J |
_version_ |
1766363228013592576 |