A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols (TAG) into fatty acid ethyl ester (FAEE) or fatty acid methyl ester (FAME) derivatives was developed. The microreactor was a porous silica monolith fabricated within a 320 μm ID fused silica capillary with lipase from...
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ftunivalberta:oai:era.library.ualberta.ca:1006a99d-e102-4bd6-bf51-63d3c69c563a 2023-05-15T13:43:19+02:00 A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis Mugo, Samuel M. Anuar, Sabiqah T. Curtis, Jonathan M. 2015 https://era.library.ualberta.ca/items/1006a99d-e102-4bd6-bf51-63d3c69c563a https://doi.org/10.7939/R3794173N English eng https://era.library.ualberta.ca/items/1006a99d-e102-4bd6-bf51-63d3c69c563a doi:10.7939/R3794173N © 2015 The Royal Society of Chemistry. This work is licensed according to the Attribution-NonCommercial 4.0 International terms of use. Biodiesel Production Silica Candida-Antarctica Lipase Sunflower Oil Lipid Transformations Vegetable-Oils Transesterification Gas-Chromatography Catalyzed Alcoholysis Liquid-Chromatography Article (Published) 2015 ftunivalberta https://doi.org/10.7939/R3794173N 2022-08-22T20:09:13Z A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols (TAG) into fatty acid ethyl ester (FAEE) or fatty acid methyl ester (FAME) derivatives was developed. The microreactor was a porous silica monolith fabricated within a 320 μm ID fused silica capillary with lipase from Candida antarctica immobilized onto the large surface area of the monolith. The microreactor was used for the room temperature ethanolysis of TAG from edible oils including canola, sesame, soybean and refined-bleached-deodorized palm oil. GC/MS-NCI and GC/FID were used to prove the identification of the FAEE and FAME products. The microreactor completely transformed the starting oils into FAEEs or FAMEs, without the use of any reagents other than alcohol, in quantities suitable for GC analysis. The prototype microreactors were reusable >5 times with ethanol and 2 times with methanol. The FAEE products obtained using the microreactor were similar to those produced using commercial Novozyme 435 enzyme beads as well as by catalysis with ethanolic H2SO4. Other/Unknown Material Antarc* Antarctica University of Alberta: Era - Education and Research Archive Fid ENVELOPE(-65.939,-65.939,-68.664,-68.664) Monolith ENVELOPE(163.283,163.283,-66.950,-66.950) Monolith The ENVELOPE(163.283,163.283,-66.950,-66.950) The Monolith ENVELOPE(-57.955,-57.955,-63.897,-63.897) |
institution |
Open Polar |
collection |
University of Alberta: Era - Education and Research Archive |
op_collection_id |
ftunivalberta |
language |
English |
topic |
Biodiesel Production Silica Candida-Antarctica Lipase Sunflower Oil Lipid Transformations Vegetable-Oils Transesterification Gas-Chromatography Catalyzed Alcoholysis Liquid-Chromatography |
spellingShingle |
Biodiesel Production Silica Candida-Antarctica Lipase Sunflower Oil Lipid Transformations Vegetable-Oils Transesterification Gas-Chromatography Catalyzed Alcoholysis Liquid-Chromatography Mugo, Samuel M. Anuar, Sabiqah T. Curtis, Jonathan M. A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
topic_facet |
Biodiesel Production Silica Candida-Antarctica Lipase Sunflower Oil Lipid Transformations Vegetable-Oils Transesterification Gas-Chromatography Catalyzed Alcoholysis Liquid-Chromatography |
description |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols (TAG) into fatty acid ethyl ester (FAEE) or fatty acid methyl ester (FAME) derivatives was developed. The microreactor was a porous silica monolith fabricated within a 320 μm ID fused silica capillary with lipase from Candida antarctica immobilized onto the large surface area of the monolith. The microreactor was used for the room temperature ethanolysis of TAG from edible oils including canola, sesame, soybean and refined-bleached-deodorized palm oil. GC/MS-NCI and GC/FID were used to prove the identification of the FAEE and FAME products. The microreactor completely transformed the starting oils into FAEEs or FAMEs, without the use of any reagents other than alcohol, in quantities suitable for GC analysis. The prototype microreactors were reusable >5 times with ethanol and 2 times with methanol. The FAEE products obtained using the microreactor were similar to those produced using commercial Novozyme 435 enzyme beads as well as by catalysis with ethanolic H2SO4. |
format |
Other/Unknown Material |
author |
Mugo, Samuel M. Anuar, Sabiqah T. Curtis, Jonathan M. |
author_facet |
Mugo, Samuel M. Anuar, Sabiqah T. Curtis, Jonathan M. |
author_sort |
Mugo, Samuel M. |
title |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_short |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_full |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_fullStr |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_full_unstemmed |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_sort |
flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for gc analysis |
publishDate |
2015 |
url |
https://era.library.ualberta.ca/items/1006a99d-e102-4bd6-bf51-63d3c69c563a https://doi.org/10.7939/R3794173N |
long_lat |
ENVELOPE(-65.939,-65.939,-68.664,-68.664) ENVELOPE(163.283,163.283,-66.950,-66.950) ENVELOPE(163.283,163.283,-66.950,-66.950) ENVELOPE(-57.955,-57.955,-63.897,-63.897) |
geographic |
Fid Monolith Monolith The The Monolith |
geographic_facet |
Fid Monolith Monolith The The Monolith |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://era.library.ualberta.ca/items/1006a99d-e102-4bd6-bf51-63d3c69c563a doi:10.7939/R3794173N |
op_rights |
© 2015 The Royal Society of Chemistry. This work is licensed according to the Attribution-NonCommercial 4.0 International terms of use. |
op_doi |
https://doi.org/10.7939/R3794173N |
_version_ |
1766187515977400320 |