Nmda Receptors In Hibernating Arctic Ground Squirrels
Dissertation (Ph.D.) University of Alaska Fairbanks, 2005 Hibernation is a unique physiological state characterized by suppressed metabolism and body temperature that is interrupted by multiple, brief periods of arousal throughout the hibernation season. Blood flow fluctuates during hibernation and...
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ftunivalaska:oai:scholarworks.alaska.edu:11122/8886 2023-05-15T15:00:55+02:00 Nmda Receptors In Hibernating Arctic Ground Squirrels Zhao, Huiwen Drew, Kelly 2005 http://hdl.handle.net/11122/8886 unknown http://hdl.handle.net/11122/8886 Department of Chemistry and Biochemistry Neurosciences Animal Physiology Zoology Dissertation phd 2005 ftunivalaska 2023-02-23T21:37:09Z Dissertation (Ph.D.) University of Alaska Fairbanks, 2005 Hibernation is a unique physiological state characterized by suppressed metabolism and body temperature that is interrupted by multiple, brief periods of arousal throughout the hibernation season. Blood flow fluctuates during hibernation and arousal in a reperfusion-like manner without causing neurological damage. Previous studies show that hippocampal slices from hibernating animals tolerate experimental oxygen nutrient deprivation and N-methyl-D-aspartate (NMDA) toxicity better than slices from euthermic animals. However, the cellular mechanisms underlying these examples of tolerance remain unclear. Tolerance to NMDA toxicity suggests that modulation of NMDA receptors (NMDAR) contributes to intrinsic tissue tolerance in slices from hibernating Arctic ground squirrels (hAGS, Spermophilus parryii). NMDAR are one subtype of glutamate receptors. NMDAR play critical roles in excitatory synaptic transmission, synaptic plasticity, learning and memory, and excitotoxicity. NMDAR1 (NR1) is a fundamental subunit of NMDAR and required for receptor function. The main focus of the current project was to test the hypothesis that NMDAR are down-regulated in hAGS compared with interbout euthermic AGS (ibeAGS) and to explore the potential mechanisms of this down-regulation. NMDAR function can be modulated by protein phosphorylation, subunit composition, and internalization. Hence, the aim of chapter 2 was to determine the distribution of NRl in hAGS and ibeAGS using immunohistochemistry. The aim of chapter 3 was to examine NMDAR function in cultured hippocampal slices from hAGS, ibeAGS, and rats using calcium imaging, and to investigate potential modulation of NMDAR such as phosphorylation and internalization for altered function using western blot analysis. Given that synaptic remodeling and functional changes after arousal from hibernation, and NMDAR play an important role in learning and memory, the aim of chapter 4 was to address the effects of hibernation on ... Doctoral or Postdoctoral Thesis Arctic Alaska University of Alaska: ScholarWorks@UA Arctic Fairbanks |
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Open Polar |
collection |
University of Alaska: ScholarWorks@UA |
op_collection_id |
ftunivalaska |
language |
unknown |
topic |
Neurosciences Animal Physiology Zoology |
spellingShingle |
Neurosciences Animal Physiology Zoology Zhao, Huiwen Nmda Receptors In Hibernating Arctic Ground Squirrels |
topic_facet |
Neurosciences Animal Physiology Zoology |
description |
Dissertation (Ph.D.) University of Alaska Fairbanks, 2005 Hibernation is a unique physiological state characterized by suppressed metabolism and body temperature that is interrupted by multiple, brief periods of arousal throughout the hibernation season. Blood flow fluctuates during hibernation and arousal in a reperfusion-like manner without causing neurological damage. Previous studies show that hippocampal slices from hibernating animals tolerate experimental oxygen nutrient deprivation and N-methyl-D-aspartate (NMDA) toxicity better than slices from euthermic animals. However, the cellular mechanisms underlying these examples of tolerance remain unclear. Tolerance to NMDA toxicity suggests that modulation of NMDA receptors (NMDAR) contributes to intrinsic tissue tolerance in slices from hibernating Arctic ground squirrels (hAGS, Spermophilus parryii). NMDAR are one subtype of glutamate receptors. NMDAR play critical roles in excitatory synaptic transmission, synaptic plasticity, learning and memory, and excitotoxicity. NMDAR1 (NR1) is a fundamental subunit of NMDAR and required for receptor function. The main focus of the current project was to test the hypothesis that NMDAR are down-regulated in hAGS compared with interbout euthermic AGS (ibeAGS) and to explore the potential mechanisms of this down-regulation. NMDAR function can be modulated by protein phosphorylation, subunit composition, and internalization. Hence, the aim of chapter 2 was to determine the distribution of NRl in hAGS and ibeAGS using immunohistochemistry. The aim of chapter 3 was to examine NMDAR function in cultured hippocampal slices from hAGS, ibeAGS, and rats using calcium imaging, and to investigate potential modulation of NMDAR such as phosphorylation and internalization for altered function using western blot analysis. Given that synaptic remodeling and functional changes after arousal from hibernation, and NMDAR play an important role in learning and memory, the aim of chapter 4 was to address the effects of hibernation on ... |
author2 |
Drew, Kelly |
format |
Doctoral or Postdoctoral Thesis |
author |
Zhao, Huiwen |
author_facet |
Zhao, Huiwen |
author_sort |
Zhao, Huiwen |
title |
Nmda Receptors In Hibernating Arctic Ground Squirrels |
title_short |
Nmda Receptors In Hibernating Arctic Ground Squirrels |
title_full |
Nmda Receptors In Hibernating Arctic Ground Squirrels |
title_fullStr |
Nmda Receptors In Hibernating Arctic Ground Squirrels |
title_full_unstemmed |
Nmda Receptors In Hibernating Arctic Ground Squirrels |
title_sort |
nmda receptors in hibernating arctic ground squirrels |
publishDate |
2005 |
url |
http://hdl.handle.net/11122/8886 |
geographic |
Arctic Fairbanks |
geographic_facet |
Arctic Fairbanks |
genre |
Arctic Alaska |
genre_facet |
Arctic Alaska |
op_relation |
http://hdl.handle.net/11122/8886 Department of Chemistry and Biochemistry |
_version_ |
1766332963959603200 |