Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA

1 liter water samples collected around Fairbanks/North Pole for eDNA analysis. Filters were cut in half and DNA isolated via phenol-chloroform from one half. Other half is archived. qPCR (SYBR Green) conducted for detection of wood frog cytB (Rasy_00). We developed and describe a qPCR assay for the...

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Bibliographic Details
Main Authors: Spangler, Mark A., Huettmann, Falk, Herriott, Ian C., López, J. Andrés
Format: Dataset
Language:English
Published: Springer 2017
Subjects:
eDNA
wood frog
Rana sylvatica
cytochrome B
Alaska
Arctic
Calvin
Fairbanks
North Pole
Institute of Arctic Biology
Online Access:http://hdl.handle.net/11122/8005

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