Development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis

Thesis (M.S.) University of Alaska Fairbanks, 2005 To elucidate the roles of key neurochemicals in the metabolic suppression of Arctic ground squirrels, a technique is required that is capable of on-line, in vivo, high temporal resolution, quantitative detection of neurotransmitters. A capillary ele...

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Main Author: Swearingen, Kristian Edward
Format: Thesis
Language:English
Published: 2005
Subjects:
Arctic
Fairbanks
Alaska
Online Access:http://hdl.handle.net/11122/6112
id ftunivalaska:oai:scholarworks.alaska.edu:11122/6112
record_format openpolar
spelling ftunivalaska:oai:scholarworks.alaska.edu:11122/6112 2023-05-15T15:00:22+02:00 Development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis Swearingen, Kristian Edward 2005-12 http://hdl.handle.net/11122/6112 en_US eng http://hdl.handle.net/11122/6112 Department of Chemistry & Biochemistry Thesis ms 2005 ftunivalaska 2023-02-23T21:36:35Z Thesis (M.S.) University of Alaska Fairbanks, 2005 To elucidate the roles of key neurochemicals in the metabolic suppression of Arctic ground squirrels, a technique is required that is capable of on-line, in vivo, high temporal resolution, quantitative detection of neurotransmitters. A capillary electrophoresis instrument has been built that is coupled to microdialysis by flow-gated injection. Primary amines recovered in vivo via microdialysis are derivatized on-column by cyanide-catalyzed formation of fluorescent isoindoles with naphthalene-2,3-dialdehyde for laser-induced fluorescence detection. The system is capable of detecting base-line glutamate in vivo in 15-second intervals. Thesis Arctic Alaska University of Alaska: ScholarWorks@UA Arctic Fairbanks
institution Open Polar
collection University of Alaska: ScholarWorks@UA
op_collection_id ftunivalaska
language English
description Thesis (M.S.) University of Alaska Fairbanks, 2005 To elucidate the roles of key neurochemicals in the metabolic suppression of Arctic ground squirrels, a technique is required that is capable of on-line, in vivo, high temporal resolution, quantitative detection of neurotransmitters. A capillary electrophoresis instrument has been built that is coupled to microdialysis by flow-gated injection. Primary amines recovered in vivo via microdialysis are derivatized on-column by cyanide-catalyzed formation of fluorescent isoindoles with naphthalene-2,3-dialdehyde for laser-induced fluorescence detection. The system is capable of detecting base-line glutamate in vivo in 15-second intervals.
format Thesis
author Swearingen, Kristian Edward
spellingShingle Swearingen, Kristian Edward
Development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis
author_facet Swearingen, Kristian Edward
author_sort Swearingen, Kristian Edward
title Development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis
title_short Development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis
title_full Development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis
title_fullStr Development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis
title_full_unstemmed Development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis
title_sort development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis
publishDate 2005
url http://hdl.handle.net/11122/6112
geographic Arctic
Fairbanks
geographic_facet Arctic
Fairbanks
genre Arctic
Alaska
genre_facet Arctic
Alaska
op_relation http://hdl.handle.net/11122/6112
Department of Chemistry & Biochemistry
_version_ 1766332466421825536

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