| Summary: | Thesis (M.S.) University of Alaska Fairbanks, 2006 Alaskan Arctic ground squirrels, Spermophilus paryii, hibernate about seven months per year. During two-week torpor periods, respiration, circulation, metabolism, and catabolism are dramatically decreased, except for brief periods of interbout euthermia. These divergent hibernation states provide a particularly compelling model for variance-based studies of global gene expression. A guiding hypothesis in this Thesis is that Arctic ground squirrels exit interbout euthermia and enter torpor with an invariant metabolic scaffolding of various metabolites that are erected to serve as a ready metabalome for the challenges of the next brief return to euthermia. To develop this hypothesis further, I performed an exploratory data analysis of high-density mouse cDNA micro arrays cross-hybridized with Arctic ground squirrel mRNA to measure transcriptomes in brown adipose, skeletal muscle, and liver tissues. The results revealed that variation in transcript expression profiles were tissue specific and may reflect the degree to which tissues are active during hibernation. These results are encouraging. They justify a more thorough evaluation of the utility of using global variation in transcript expression patterns. In combination with a priori biological knowledge, these patterns will guide future studies into more detailed analyses of hibernation-state dependent and functionally relevant transcripts.
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