Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients
International audience Abstract Polar regions are relatively isolated from human activity and thus could offer insight into anthropogenic and ecological drivers of the spread of antibiotic resistance. Plasmids are of particular interest in this context given the central role that they are thought to...
Published in: | Global Change Biology |
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Online Access: | https://hal.science/hal-04621011 https://hal.science/hal-04621011/document https://hal.science/hal-04621011/file/Makowska-Zawierucha%20Global%20Change%20Biology%20-%202024%20-%20Arctic%20plasmidome%20analysis%20reveals%20distinct%20relationships%20among.pdf https://doi.org/10.1111/gcb.17293 |
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ftunivaixmarseil:oai:HAL:hal-04621011v1 2024-09-15T17:52:14+00:00 Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients Makowska‐zawierucha, Nicoletta Trzebny, Artur Zawierucha, Krzysztof Manthapuri, Vineeth Bradley, James, A Pruden, Amy Institut méditerranéen d'océanologie (MIO) Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS) Queen Mary University of London (QMUL) 2024-04-30 https://hal.science/hal-04621011 https://hal.science/hal-04621011/document https://hal.science/hal-04621011/file/Makowska-Zawierucha%20Global%20Change%20Biology%20-%202024%20-%20Arctic%20plasmidome%20analysis%20reveals%20distinct%20relationships%20among.pdf https://doi.org/10.1111/gcb.17293 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/gcb.17293 hal-04621011 https://hal.science/hal-04621011 https://hal.science/hal-04621011/document https://hal.science/hal-04621011/file/Makowska-Zawierucha%20Global%20Change%20Biology%20-%202024%20-%20Arctic%20plasmidome%20analysis%20reveals%20distinct%20relationships%20among.pdf doi:10.1111/gcb.17293 info:eu-repo/semantics/OpenAccess ISSN: 1354-1013 EISSN: 1365-2486 Global Change Biology https://hal.science/hal-04621011 Global Change Biology, 2024, 30 (5), ⟨10.1111/gcb.17293⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2024 ftunivaixmarseil https://doi.org/10.1111/gcb.17293 2024-08-07T23:37:47Z International audience Abstract Polar regions are relatively isolated from human activity and thus could offer insight into anthropogenic and ecological drivers of the spread of antibiotic resistance. Plasmids are of particular interest in this context given the central role that they are thought to play in the dissemination of antibiotic resistance genes (ARGs). However, plasmidomes are challenging to profile in environmental samples. The objective of this study was to compare various aspects of the plasmidome associated with glacial ice and adjacent aquatic environments across the high Arctic archipelago of Svalbard, representing a gradient of anthropogenic inputs and specific treated and untreated wastewater outflows to the sea. We accessed plasmidomes by applying enrichment cultures, plasmid isolation and shotgun Illumina sequencing of environmental samples. We examined the abundance and diversity of ARGs and other stress‐response genes that might be co/cross‐selected or co‐transported in these environments, including biocide resistance genes (BRGs), metal resistance genes (MRGs), virulence genes (VGs) and integrons. We found striking differences between glacial ice and aquatic environments in terms of the ARGs carried by plasmids. We found a strong correlation between MRGs and ARGs in plasmids in the wastewaters and fjords. Alternatively, in glacial ice, VGs and BRGs genes were dominant, suggesting that glacial ice may be a repository of pathogenic strains. Moreover, ARGs were not found within the cassettes of integrons carried by the plasmids, which is suggestive of unique adaptive features of the microbial communities to their extreme environment. This study provides insight into the role of plasmids in facilitating bacterial adaptation to Arctic ecosystems as well as in shaping corresponding resistomes. Increasing human activity, warming of Arctic regions and associated increases in the meltwater run‐off from glaciers could contribute to the release and spread of plasmid‐related genes from Svalbard to ... Article in Journal/Newspaper Arctic Archipelago Svalbard Aix-Marseille Université: HAL Global Change Biology 30 5 |
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Open Polar |
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Aix-Marseille Université: HAL |
op_collection_id |
ftunivaixmarseil |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Makowska‐zawierucha, Nicoletta Trzebny, Artur Zawierucha, Krzysztof Manthapuri, Vineeth Bradley, James, A Pruden, Amy Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience Abstract Polar regions are relatively isolated from human activity and thus could offer insight into anthropogenic and ecological drivers of the spread of antibiotic resistance. Plasmids are of particular interest in this context given the central role that they are thought to play in the dissemination of antibiotic resistance genes (ARGs). However, plasmidomes are challenging to profile in environmental samples. The objective of this study was to compare various aspects of the plasmidome associated with glacial ice and adjacent aquatic environments across the high Arctic archipelago of Svalbard, representing a gradient of anthropogenic inputs and specific treated and untreated wastewater outflows to the sea. We accessed plasmidomes by applying enrichment cultures, plasmid isolation and shotgun Illumina sequencing of environmental samples. We examined the abundance and diversity of ARGs and other stress‐response genes that might be co/cross‐selected or co‐transported in these environments, including biocide resistance genes (BRGs), metal resistance genes (MRGs), virulence genes (VGs) and integrons. We found striking differences between glacial ice and aquatic environments in terms of the ARGs carried by plasmids. We found a strong correlation between MRGs and ARGs in plasmids in the wastewaters and fjords. Alternatively, in glacial ice, VGs and BRGs genes were dominant, suggesting that glacial ice may be a repository of pathogenic strains. Moreover, ARGs were not found within the cassettes of integrons carried by the plasmids, which is suggestive of unique adaptive features of the microbial communities to their extreme environment. This study provides insight into the role of plasmids in facilitating bacterial adaptation to Arctic ecosystems as well as in shaping corresponding resistomes. Increasing human activity, warming of Arctic regions and associated increases in the meltwater run‐off from glaciers could contribute to the release and spread of plasmid‐related genes from Svalbard to ... |
author2 |
Institut méditerranéen d'océanologie (MIO) Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS) Queen Mary University of London (QMUL) |
format |
Article in Journal/Newspaper |
author |
Makowska‐zawierucha, Nicoletta Trzebny, Artur Zawierucha, Krzysztof Manthapuri, Vineeth Bradley, James, A Pruden, Amy |
author_facet |
Makowska‐zawierucha, Nicoletta Trzebny, Artur Zawierucha, Krzysztof Manthapuri, Vineeth Bradley, James, A Pruden, Amy |
author_sort |
Makowska‐zawierucha, Nicoletta |
title |
Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients |
title_short |
Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients |
title_full |
Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients |
title_fullStr |
Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients |
title_full_unstemmed |
Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients |
title_sort |
arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients |
publisher |
HAL CCSD |
publishDate |
2024 |
url |
https://hal.science/hal-04621011 https://hal.science/hal-04621011/document https://hal.science/hal-04621011/file/Makowska-Zawierucha%20Global%20Change%20Biology%20-%202024%20-%20Arctic%20plasmidome%20analysis%20reveals%20distinct%20relationships%20among.pdf https://doi.org/10.1111/gcb.17293 |
genre |
Arctic Archipelago Svalbard |
genre_facet |
Arctic Archipelago Svalbard |
op_source |
ISSN: 1354-1013 EISSN: 1365-2486 Global Change Biology https://hal.science/hal-04621011 Global Change Biology, 2024, 30 (5), ⟨10.1111/gcb.17293⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/gcb.17293 hal-04621011 https://hal.science/hal-04621011 https://hal.science/hal-04621011/document https://hal.science/hal-04621011/file/Makowska-Zawierucha%20Global%20Change%20Biology%20-%202024%20-%20Arctic%20plasmidome%20analysis%20reveals%20distinct%20relationships%20among.pdf doi:10.1111/gcb.17293 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1111/gcb.17293 |
container_title |
Global Change Biology |
container_volume |
30 |
container_issue |
5 |
_version_ |
1810294293530673152 |