Past and present giant viruses diversity explored through permafrost metagenomics
International audience Giant viruses are abundant in aquatic environments and ecologically important through the metabolic reprogramming of their hosts. Less is known about giant viruses from soil even though two of them, belonging to two different viral families, were reactivated from 30,000-y-old...
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ftunivaixmarseil:oai:HAL:hal-03807788v1 2024-05-12T08:09:46+00:00 Past and present giant viruses diversity explored through permafrost metagenomics Rigou, Sofia Santini, Sébastien Abergel, Chantal Claverie, Jean-Michel Legendre, Matthieu Information génomique et structurale (IGS) Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) 2022-10-07 https://hal.science/hal-03807788 https://hal.science/hal-03807788/document https://hal.science/hal-03807788/file/s41467-022-33633-x.pdf https://doi.org/10.1038/s41467-022-33633-x en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-022-33633-x hal-03807788 https://hal.science/hal-03807788 https://hal.science/hal-03807788/document https://hal.science/hal-03807788/file/s41467-022-33633-x.pdf doi:10.1038/s41467-022-33633-x http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 2041-1723 EISSN: 2041-1723 Nature Communications https://hal.science/hal-03807788 Nature Communications, 2022, 13 (1), pp.5853. ⟨10.1038/s41467-022-33633-x⟩ Metagenomics Phylogenetics Phylogeny Soil microbiology [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2022 ftunivaixmarseil https://doi.org/10.1038/s41467-022-33633-x 2024-04-18T00:31:06Z International audience Giant viruses are abundant in aquatic environments and ecologically important through the metabolic reprogramming of their hosts. Less is known about giant viruses from soil even though two of them, belonging to two different viral families, were reactivated from 30,000-y-old permafrost samples. This suggests an untapped diversity of Nucleocytoviricota in this environment. Through permafrost metagenomics we reveal a unique diversity pattern and a high heterogeneity in the abundance of giant viruses, representing up to 12% of the sum of sequence coverage in one sample. Pithoviridae and Orpheoviridae -like viruses were the most important contributors. A complete 1.6 Mb Pithoviridae -like circular genome was also assembled from a 42,000-y-old sample. The annotation of the permafrost viral sequences revealed a patchwork of predicted functions amidst a larger reservoir of genes of unknown functions. Finally, the phylogenetic reconstructions not only revealed gene transfers between cells and viruses, but also between viruses from different families. Article in Journal/Newspaper permafrost Aix-Marseille Université: HAL Nature Communications 13 1 |
institution |
Open Polar |
collection |
Aix-Marseille Université: HAL |
op_collection_id |
ftunivaixmarseil |
language |
English |
topic |
Metagenomics Phylogenetics Phylogeny Soil microbiology [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences |
spellingShingle |
Metagenomics Phylogenetics Phylogeny Soil microbiology [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences Rigou, Sofia Santini, Sébastien Abergel, Chantal Claverie, Jean-Michel Legendre, Matthieu Past and present giant viruses diversity explored through permafrost metagenomics |
topic_facet |
Metagenomics Phylogenetics Phylogeny Soil microbiology [SDV]Life Sciences [q-bio] [SDE]Environmental Sciences |
description |
International audience Giant viruses are abundant in aquatic environments and ecologically important through the metabolic reprogramming of their hosts. Less is known about giant viruses from soil even though two of them, belonging to two different viral families, were reactivated from 30,000-y-old permafrost samples. This suggests an untapped diversity of Nucleocytoviricota in this environment. Through permafrost metagenomics we reveal a unique diversity pattern and a high heterogeneity in the abundance of giant viruses, representing up to 12% of the sum of sequence coverage in one sample. Pithoviridae and Orpheoviridae -like viruses were the most important contributors. A complete 1.6 Mb Pithoviridae -like circular genome was also assembled from a 42,000-y-old sample. The annotation of the permafrost viral sequences revealed a patchwork of predicted functions amidst a larger reservoir of genes of unknown functions. Finally, the phylogenetic reconstructions not only revealed gene transfers between cells and viruses, but also between viruses from different families. |
author2 |
Information génomique et structurale (IGS) Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Rigou, Sofia Santini, Sébastien Abergel, Chantal Claverie, Jean-Michel Legendre, Matthieu |
author_facet |
Rigou, Sofia Santini, Sébastien Abergel, Chantal Claverie, Jean-Michel Legendre, Matthieu |
author_sort |
Rigou, Sofia |
title |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_short |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_full |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_fullStr |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_full_unstemmed |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_sort |
past and present giant viruses diversity explored through permafrost metagenomics |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://hal.science/hal-03807788 https://hal.science/hal-03807788/document https://hal.science/hal-03807788/file/s41467-022-33633-x.pdf https://doi.org/10.1038/s41467-022-33633-x |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
ISSN: 2041-1723 EISSN: 2041-1723 Nature Communications https://hal.science/hal-03807788 Nature Communications, 2022, 13 (1), pp.5853. ⟨10.1038/s41467-022-33633-x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-022-33633-x hal-03807788 https://hal.science/hal-03807788 https://hal.science/hal-03807788/document https://hal.science/hal-03807788/file/s41467-022-33633-x.pdf doi:10.1038/s41467-022-33633-x |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1038/s41467-022-33633-x |
container_title |
Nature Communications |
container_volume |
13 |
container_issue |
1 |
_version_ |
1798853085487955968 |