Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome
Wild relatives of wheat are important resources for new rust resistance genes but underutilized because the valuable resistances are often linked to negative traits that prevent deployment of these genes in commercial wheats. Here, we report ph1b-induced recombinants with reduced alien chromatin der...
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/87140 2023-12-17T10:49:33+01:00 Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome Mago, R. Verlin, D. Zhang, P. Bansal, U. Bariana, H. Jin, Y. Ellis, J. Hoxha, S. Dundas, I. 2013 http://hdl.handle.net/2440/87140 https://doi.org/10.1007/s00122-013-2184-8 en eng Springer-Verlag Theoretical and Applied Genetics: international journal of plant breeding research, 2013; 126(12):2943-2955 0040-5752 1432-2242 http://hdl.handle.net/2440/87140 doi:10.1007/s00122-013-2184-8 © Springer-Verlag Berlin Heidelberg 2013 http://dx.doi.org/10.1007/s00122-013-2184-8 Chromosomes Plant Chromatin Basidiomycota Triticum Plant Stems Genetic Markers In Situ Hybridization Fluorescence Polymerase Chain Reaction Plant Diseases Genes Homologous Recombination Journal article 2013 ftunivadelaidedl https://doi.org/10.1007/s00122-013-2184-8 2023-11-20T23:32:07Z Wild relatives of wheat are important resources for new rust resistance genes but underutilized because the valuable resistances are often linked to negative traits that prevent deployment of these genes in commercial wheats. Here, we report ph1b-induced recombinants with reduced alien chromatin derived from E.R. Sears’ wheat–Aegilops speltoides 2D-2S#1 translocation line C82.2, which carries the widely effective stem rust resistance gene Sr32. Infection type assessments of the recombinants showed that the original translocation in fact carries two stem rust resistance genes, Sr32 on the short arm and a previously undescribed gene SrAes1t on the long arm of chromosome 2S#1. Recombinants with substantially shortened alien chromatin were produced for both genes, which confer resistance to stem rust races in the TTKSK (Ug99) lineage and representative races of all Australian stem rust lineages. Selected recombinants were back crossed into adapted Australian cultivars and PCR markers were developed to facilitate the incorporation of these genes into future wheat varieties. Our recombinants and those from several other labs now show that Sr32, Sr39, and SrAes7t on the short arm and Sr47 and SrAes1t on the long arm of 2S#1 form two linkage groups and at present no rust races are described that can distinguish these resistance specificities. Rohit Mago, Dawn Verlin, Peng Zhang, Urmil Bansal, Harbans Bariana, Yue Jin, Jeffrey Ellis, Sami Hoxha, Ian Dundas Article in Journal/Newspaper sami The University of Adelaide: Digital Library Long Arm ENVELOPE(-55.648,-55.648,49.517,49.517) Theoretical and Applied Genetics 126 12 2943 2955 |
institution |
Open Polar |
collection |
The University of Adelaide: Digital Library |
op_collection_id |
ftunivadelaidedl |
language |
English |
topic |
Chromosomes Plant Chromatin Basidiomycota Triticum Plant Stems Genetic Markers In Situ Hybridization Fluorescence Polymerase Chain Reaction Plant Diseases Genes Homologous Recombination |
spellingShingle |
Chromosomes Plant Chromatin Basidiomycota Triticum Plant Stems Genetic Markers In Situ Hybridization Fluorescence Polymerase Chain Reaction Plant Diseases Genes Homologous Recombination Mago, R. Verlin, D. Zhang, P. Bansal, U. Bariana, H. Jin, Y. Ellis, J. Hoxha, S. Dundas, I. Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome |
topic_facet |
Chromosomes Plant Chromatin Basidiomycota Triticum Plant Stems Genetic Markers In Situ Hybridization Fluorescence Polymerase Chain Reaction Plant Diseases Genes Homologous Recombination |
description |
Wild relatives of wheat are important resources for new rust resistance genes but underutilized because the valuable resistances are often linked to negative traits that prevent deployment of these genes in commercial wheats. Here, we report ph1b-induced recombinants with reduced alien chromatin derived from E.R. Sears’ wheat–Aegilops speltoides 2D-2S#1 translocation line C82.2, which carries the widely effective stem rust resistance gene Sr32. Infection type assessments of the recombinants showed that the original translocation in fact carries two stem rust resistance genes, Sr32 on the short arm and a previously undescribed gene SrAes1t on the long arm of chromosome 2S#1. Recombinants with substantially shortened alien chromatin were produced for both genes, which confer resistance to stem rust races in the TTKSK (Ug99) lineage and representative races of all Australian stem rust lineages. Selected recombinants were back crossed into adapted Australian cultivars and PCR markers were developed to facilitate the incorporation of these genes into future wheat varieties. Our recombinants and those from several other labs now show that Sr32, Sr39, and SrAes7t on the short arm and Sr47 and SrAes1t on the long arm of 2S#1 form two linkage groups and at present no rust races are described that can distinguish these resistance specificities. Rohit Mago, Dawn Verlin, Peng Zhang, Urmil Bansal, Harbans Bariana, Yue Jin, Jeffrey Ellis, Sami Hoxha, Ian Dundas |
format |
Article in Journal/Newspaper |
author |
Mago, R. Verlin, D. Zhang, P. Bansal, U. Bariana, H. Jin, Y. Ellis, J. Hoxha, S. Dundas, I. |
author_facet |
Mago, R. Verlin, D. Zhang, P. Bansal, U. Bariana, H. Jin, Y. Ellis, J. Hoxha, S. Dundas, I. |
author_sort |
Mago, R. |
title |
Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome |
title_short |
Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome |
title_full |
Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome |
title_fullStr |
Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome |
title_full_unstemmed |
Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome |
title_sort |
development of wheat-aegilops speltoides recombinants and simple pcr-based markers for sr32 and a new stem rust resistance gene on the 2s#1 chromosome |
publisher |
Springer-Verlag |
publishDate |
2013 |
url |
http://hdl.handle.net/2440/87140 https://doi.org/10.1007/s00122-013-2184-8 |
long_lat |
ENVELOPE(-55.648,-55.648,49.517,49.517) |
geographic |
Long Arm |
geographic_facet |
Long Arm |
genre |
sami |
genre_facet |
sami |
op_source |
http://dx.doi.org/10.1007/s00122-013-2184-8 |
op_relation |
Theoretical and Applied Genetics: international journal of plant breeding research, 2013; 126(12):2943-2955 0040-5752 1432-2242 http://hdl.handle.net/2440/87140 doi:10.1007/s00122-013-2184-8 |
op_rights |
© Springer-Verlag Berlin Heidelberg 2013 |
op_doi |
https://doi.org/10.1007/s00122-013-2184-8 |
container_title |
Theoretical and Applied Genetics |
container_volume |
126 |
container_issue |
12 |
container_start_page |
2943 |
op_container_end_page |
2955 |
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1785574045760094208 |