Susceptibility of juvenile European lobster Homarus gammarus to shrimp products infected with high and low doses of white spot syndrome virus
White spot syndrome virus (WSSV) is the most important pathogen known to affect the sustainability and growth of the global penaeid shrimp farming industry. Although most commonly associated with penaeid shrimp farmed in warm waters, WSSV is also able to infect, cause disease in and kill a wide rang...
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/73611 2023-12-24T10:16:29+01:00 Susceptibility of juvenile European lobster Homarus gammarus to shrimp products infected with high and low doses of white spot syndrome virus Bateman, K. Munro, J. Uglow, B. Small, H. Stentiford, G. 2012 http://hdl.handle.net/2440/73611 https://doi.org/10.3354/dao02474 en eng Inter-research Diseases of Aquatic Organisms, 2012; 100(2):169-184 0177-5103 1616-1580 http://hdl.handle.net/2440/73611 doi:10.3354/dao02474 © The Crown 2012 http://dx.doi.org/10.3354/dao02474 White spot syndrome virus WSSV commodity transmission risk assessment Journal article 2012 ftunivadelaidedl https://doi.org/10.3354/dao02474 2023-11-27T23:23:43Z White spot syndrome virus (WSSV) is the most important pathogen known to affect the sustainability and growth of the global penaeid shrimp farming industry. Although most commonly associated with penaeid shrimp farmed in warm waters, WSSV is also able to infect, cause disease in and kill a wide range of other decapod crustaceans, including lobsters, from temperate regions. In 2005, the European Union imported US$500 million worth of raw frozen or cooked frozen commodity products, much of which originated in regions positive for white spot disease (WSD). The presence of WSSV within the UK food market was verified by means of nested PCR performed on samples collected from a small-scale survey of supermarket commodity shrimp. Passage trials using inoculum derived from commodity shrimp from supermarkets and delivered by injection to specific pathogen-free Pacific white shrimp Litopenaeus vannamei led to rapid mortality and pathognomonic signs of WSD in the shrimp, demonstrating that WSSV present within commodity shrimp was viable. We exposed a representative European decapod crustacean, the European lobster Homarus gammarus, to a single feeding of WSSV-positive, supermarket-derived commodity shrimp, and to positive control material (L. vannamei infected with a high dose of WSSV). These trials demonstrated that lobsters fed positive control (high dose) frozen raw products succumbed to WSD and displayed pathognomonic signs associated with the disease as determined by means of histology and transmission electron microscopy. Lobsters fed WSSV-positive, supermarket-derived commodity shrimp (low dose) did not succumb to WSD (no mortality or pathognomonic signs of WSD) but demonstrated a low level or latent infection via PCR. This study confirms susceptibility of H. gammarus to WSSV via single feedings of previously frozen raw shrimp products obtained directly from supermarkets. K.S. Bateman, J. Munro, B. Uglow, H.J. Small and G.D. Stentiford Article in Journal/Newspaper European lobster Homarus gammarus The University of Adelaide: Digital Library Pacific Diseases of Aquatic Organisms 100 2 169 184 |
institution |
Open Polar |
collection |
The University of Adelaide: Digital Library |
op_collection_id |
ftunivadelaidedl |
language |
English |
topic |
White spot syndrome virus WSSV commodity transmission risk assessment |
spellingShingle |
White spot syndrome virus WSSV commodity transmission risk assessment Bateman, K. Munro, J. Uglow, B. Small, H. Stentiford, G. Susceptibility of juvenile European lobster Homarus gammarus to shrimp products infected with high and low doses of white spot syndrome virus |
topic_facet |
White spot syndrome virus WSSV commodity transmission risk assessment |
description |
White spot syndrome virus (WSSV) is the most important pathogen known to affect the sustainability and growth of the global penaeid shrimp farming industry. Although most commonly associated with penaeid shrimp farmed in warm waters, WSSV is also able to infect, cause disease in and kill a wide range of other decapod crustaceans, including lobsters, from temperate regions. In 2005, the European Union imported US$500 million worth of raw frozen or cooked frozen commodity products, much of which originated in regions positive for white spot disease (WSD). The presence of WSSV within the UK food market was verified by means of nested PCR performed on samples collected from a small-scale survey of supermarket commodity shrimp. Passage trials using inoculum derived from commodity shrimp from supermarkets and delivered by injection to specific pathogen-free Pacific white shrimp Litopenaeus vannamei led to rapid mortality and pathognomonic signs of WSD in the shrimp, demonstrating that WSSV present within commodity shrimp was viable. We exposed a representative European decapod crustacean, the European lobster Homarus gammarus, to a single feeding of WSSV-positive, supermarket-derived commodity shrimp, and to positive control material (L. vannamei infected with a high dose of WSSV). These trials demonstrated that lobsters fed positive control (high dose) frozen raw products succumbed to WSD and displayed pathognomonic signs associated with the disease as determined by means of histology and transmission electron microscopy. Lobsters fed WSSV-positive, supermarket-derived commodity shrimp (low dose) did not succumb to WSD (no mortality or pathognomonic signs of WSD) but demonstrated a low level or latent infection via PCR. This study confirms susceptibility of H. gammarus to WSSV via single feedings of previously frozen raw shrimp products obtained directly from supermarkets. K.S. Bateman, J. Munro, B. Uglow, H.J. Small and G.D. Stentiford |
format |
Article in Journal/Newspaper |
author |
Bateman, K. Munro, J. Uglow, B. Small, H. Stentiford, G. |
author_facet |
Bateman, K. Munro, J. Uglow, B. Small, H. Stentiford, G. |
author_sort |
Bateman, K. |
title |
Susceptibility of juvenile European lobster Homarus gammarus to shrimp products infected with high and low doses of white spot syndrome virus |
title_short |
Susceptibility of juvenile European lobster Homarus gammarus to shrimp products infected with high and low doses of white spot syndrome virus |
title_full |
Susceptibility of juvenile European lobster Homarus gammarus to shrimp products infected with high and low doses of white spot syndrome virus |
title_fullStr |
Susceptibility of juvenile European lobster Homarus gammarus to shrimp products infected with high and low doses of white spot syndrome virus |
title_full_unstemmed |
Susceptibility of juvenile European lobster Homarus gammarus to shrimp products infected with high and low doses of white spot syndrome virus |
title_sort |
susceptibility of juvenile european lobster homarus gammarus to shrimp products infected with high and low doses of white spot syndrome virus |
publisher |
Inter-research |
publishDate |
2012 |
url |
http://hdl.handle.net/2440/73611 https://doi.org/10.3354/dao02474 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
European lobster Homarus gammarus |
genre_facet |
European lobster Homarus gammarus |
op_source |
http://dx.doi.org/10.3354/dao02474 |
op_relation |
Diseases of Aquatic Organisms, 2012; 100(2):169-184 0177-5103 1616-1580 http://hdl.handle.net/2440/73611 doi:10.3354/dao02474 |
op_rights |
© The Crown 2012 |
op_doi |
https://doi.org/10.3354/dao02474 |
container_title |
Diseases of Aquatic Organisms |
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100 |
container_issue |
2 |
container_start_page |
169 |
op_container_end_page |
184 |
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1786204099295838208 |