Eggshell Permeability: A Standard Technique for Determining Interspecific Rates of Water Vapor Conductance
Typically, eggshell water vapor conductance is measured on whole eggs, freshly collected at the commencement of a study. At times, however, it may not be possible to obtain whole fresh eggs but rather egg fragments or previously blown eggs. Here we evaluate and describe in detail a technique for mod...
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Online Access: | http://hdl.handle.net/2440/67158 https://doi.org/10.1086/656287 |
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/67158 2023-05-15T18:49:33+02:00 Eggshell Permeability: A Standard Technique for Determining Interspecific Rates of Water Vapor Conductance Portugal, S. Maurer, G. Cassey, P. 2010 http://hdl.handle.net/2440/67158 https://doi.org/10.1086/656287 en eng Univ Chicago Press Physiological and Biochemical Zoology, 2010; 83(6):1023-1031 1522-2152 1537-5293 http://hdl.handle.net/2440/67158 doi:10.1086/656287 Cassey, P. [0000-0002-2626-0172] © 2010 by The University of Chicago. All rights reserved. Egg Shell Animals Ducks Galliformes Chickens Water Permeability Journal article 2010 ftunivadelaidedl https://doi.org/10.1086/656287 2023-02-05T19:50:09Z Typically, eggshell water vapor conductance is measured on whole eggs, freshly collected at the commencement of a study. At times, however, it may not be possible to obtain whole fresh eggs but rather egg fragments or previously blown eggs. Here we evaluate and describe in detail a technique for modern laboratory analysis of eggshell conductance that uses fragments from fresh and museum eggs to determine eggshell water vapor conductance. We used fresh unincubated eggs of domesticated chickens (Gallus gallus domesticus), ducks (Anas platyrhynchos domesticus), and guinea fowl (Numida meleagris) to investigate the reliability, validity, and repeatability of the technique. To assess the suitability of museum samples, museum and freshly collected black-headed gull eggs (Larus ridibundus) were used. Fragments were cut out of the eggshell from the blunt end (B), equator (E), and pointy end (P). Eggshell fragments were glued to the top of a 0.25-mL micro test tube (Eppendorf) filled with 200 μL of distilled water and placed in a desiccator at 25°C. Eppendorfs were weighed three times at 24-h intervals, and mass loss was assumed to be a result of water evaporation. We report the following results: (1) mass loss between weighing sessions was highly repeatable and consistent in all species; (2) the majority of intraspecific variability in eggshell water vapor conductance between different eggs of the same species was explained through the differences in water vapor conductance between the three eggshell parts of the same egg (B, E, and P); (3) the technique was sensitive enough to detect significant differences between the three domestic species; (4) there was no overall significant difference between water vapor conductance of museum and fresh black-headed gull eggs; (5) there was no significant difference in water vapor conductance for egg fragments taken from the same egg both between different trials and within the same trial. We conclude, therefore, that this technique is an effective way of measuring interspecific ... Article in Journal/Newspaper Black-headed Gull Larus ridibundus The University of Adelaide: Digital Library Physiological and Biochemical Zoology 83 6 1023 1031 |
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Open Polar |
collection |
The University of Adelaide: Digital Library |
op_collection_id |
ftunivadelaidedl |
language |
English |
topic |
Egg Shell Animals Ducks Galliformes Chickens Water Permeability |
spellingShingle |
Egg Shell Animals Ducks Galliformes Chickens Water Permeability Portugal, S. Maurer, G. Cassey, P. Eggshell Permeability: A Standard Technique for Determining Interspecific Rates of Water Vapor Conductance |
topic_facet |
Egg Shell Animals Ducks Galliformes Chickens Water Permeability |
description |
Typically, eggshell water vapor conductance is measured on whole eggs, freshly collected at the commencement of a study. At times, however, it may not be possible to obtain whole fresh eggs but rather egg fragments or previously blown eggs. Here we evaluate and describe in detail a technique for modern laboratory analysis of eggshell conductance that uses fragments from fresh and museum eggs to determine eggshell water vapor conductance. We used fresh unincubated eggs of domesticated chickens (Gallus gallus domesticus), ducks (Anas platyrhynchos domesticus), and guinea fowl (Numida meleagris) to investigate the reliability, validity, and repeatability of the technique. To assess the suitability of museum samples, museum and freshly collected black-headed gull eggs (Larus ridibundus) were used. Fragments were cut out of the eggshell from the blunt end (B), equator (E), and pointy end (P). Eggshell fragments were glued to the top of a 0.25-mL micro test tube (Eppendorf) filled with 200 μL of distilled water and placed in a desiccator at 25°C. Eppendorfs were weighed three times at 24-h intervals, and mass loss was assumed to be a result of water evaporation. We report the following results: (1) mass loss between weighing sessions was highly repeatable and consistent in all species; (2) the majority of intraspecific variability in eggshell water vapor conductance between different eggs of the same species was explained through the differences in water vapor conductance between the three eggshell parts of the same egg (B, E, and P); (3) the technique was sensitive enough to detect significant differences between the three domestic species; (4) there was no overall significant difference between water vapor conductance of museum and fresh black-headed gull eggs; (5) there was no significant difference in water vapor conductance for egg fragments taken from the same egg both between different trials and within the same trial. We conclude, therefore, that this technique is an effective way of measuring interspecific ... |
format |
Article in Journal/Newspaper |
author |
Portugal, S. Maurer, G. Cassey, P. |
author_facet |
Portugal, S. Maurer, G. Cassey, P. |
author_sort |
Portugal, S. |
title |
Eggshell Permeability: A Standard Technique for Determining Interspecific Rates of Water Vapor Conductance |
title_short |
Eggshell Permeability: A Standard Technique for Determining Interspecific Rates of Water Vapor Conductance |
title_full |
Eggshell Permeability: A Standard Technique for Determining Interspecific Rates of Water Vapor Conductance |
title_fullStr |
Eggshell Permeability: A Standard Technique for Determining Interspecific Rates of Water Vapor Conductance |
title_full_unstemmed |
Eggshell Permeability: A Standard Technique for Determining Interspecific Rates of Water Vapor Conductance |
title_sort |
eggshell permeability: a standard technique for determining interspecific rates of water vapor conductance |
publisher |
Univ Chicago Press |
publishDate |
2010 |
url |
http://hdl.handle.net/2440/67158 https://doi.org/10.1086/656287 |
genre |
Black-headed Gull Larus ridibundus |
genre_facet |
Black-headed Gull Larus ridibundus |
op_relation |
Physiological and Biochemical Zoology, 2010; 83(6):1023-1031 1522-2152 1537-5293 http://hdl.handle.net/2440/67158 doi:10.1086/656287 Cassey, P. [0000-0002-2626-0172] |
op_rights |
© 2010 by The University of Chicago. All rights reserved. |
op_doi |
https://doi.org/10.1086/656287 |
container_title |
Physiological and Biochemical Zoology |
container_volume |
83 |
container_issue |
6 |
container_start_page |
1023 |
op_container_end_page |
1031 |
_version_ |
1766243154743263232 |