Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels
This paper describes the development and validation of a commercially available radioimmunoassay (RIA) for the detection of fish insulin-like growth factor-I (IGF-I). The assay was developed using recombinant barramundi IGF-I as antigen and recombinant tuna IGF-I as radiolabelled tracer and standard...
Published in: | General and Comparative Endocrinology |
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Format: | Article in Journal/Newspaper |
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Online Access: | http://hdl.handle.net/2440/55192 https://doi.org/10.1016/j.ygcen.2003.10.002 |
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/55192 2023-05-15T15:33:01+02:00 Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels Dyer, Anthony R. Upton, Zee Stone, David Alan Joseph Thomas, Philip Mark Soole, Kathleen Lydia Higgs, Naomi Quinn, K. J. Carragher, John F. School of Earth and Environmental Sciences : Ecology and Evolutionary Biology 2004 http://hdl.handle.net/2440/55192 https://doi.org/10.1016/j.ygcen.2003.10.002 en eng Elsevier General and Comparative Endocrinology, 2004; 135(3):268-275 0016-6480 http://hdl.handle.net/2440/55192 doi:10.1016/j.ygcen.2003.10.002 Journal article 2004 ftunivadelaidedl https://doi.org/10.1016/j.ygcen.2003.10.002 2023-02-05T19:42:19Z This paper describes the development and validation of a commercially available radioimmunoassay (RIA) for the detection of fish insulin-like growth factor-I (IGF-I). The assay was developed using recombinant barramundi IGF-I as antigen and recombinant tuna IGF-I as radiolabelled tracer and standard. Assay sensitivity was 0.15 ng/ml, inter-assay variation was 16% (n=9) and intra-assay variation was 3% (n=10). Cross reactivity of less than 0.01% was found with salmon insulin, salmon IGF-II and barramundi IGF-II, less than 0.5% with human IGF-I and less than 1% with human IGF-II. Parallel dose-response inhibition curves were shown for barramundi (Lates calcarifer), coho salmon (Oncorhynchus kisutch), Southern Bluefin tuna (Thunnus maccoyii), tilapia (Oreochromis mossambicus), and seabream (Pagrus auratus) IGF-I. The assay was then used to measure stress related changes in different aquacultured fish species. Salt water acclimated Atlantic salmon smolts (Salmo salar) bathed for 2 h in fresh water showed significantly lower IGF-I concentrations than control smolts two days after the bath (53.1 compared to 32.1 ng/ml), with levels of IGF-I also lower in smolts exhibiting stunted growth (stunts). Capture and confinement of wild tuna in sea-cages resulted in a significant decrease in IGF-I levels (28 ng/ml) when compared to tuna captured and sampled immediately (48 ng/ml), but had recovered to starting levels after 3 weeks (43 ng/ml). Handling and isolation in silver perch (Bidyanus bidyanus) led to a gradual decline in IGF-I over a 12 h period (36–19 ng/ml) but showed signs of recovery by 24 h (24 ng/ml) and had recovered fully 72 h after treatment (40 ng/ml). A similar trial in black bream (Acanthopagrus butcherii) showed comparable results with IGF-I levels gradually decreasing (40–26 ng/ml) over 24 h, results that were mirrored by cortisol concentrations which increased during this time (1–26 ng/ml). In the studies presented here changes in IGF-I levels were not observed for at least 3 h after exposure to the ... Article in Journal/Newspaper Atlantic salmon Salmo salar The University of Adelaide: Digital Library General and Comparative Endocrinology 135 3 268 275 |
institution |
Open Polar |
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The University of Adelaide: Digital Library |
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ftunivadelaidedl |
language |
English |
description |
This paper describes the development and validation of a commercially available radioimmunoassay (RIA) for the detection of fish insulin-like growth factor-I (IGF-I). The assay was developed using recombinant barramundi IGF-I as antigen and recombinant tuna IGF-I as radiolabelled tracer and standard. Assay sensitivity was 0.15 ng/ml, inter-assay variation was 16% (n=9) and intra-assay variation was 3% (n=10). Cross reactivity of less than 0.01% was found with salmon insulin, salmon IGF-II and barramundi IGF-II, less than 0.5% with human IGF-I and less than 1% with human IGF-II. Parallel dose-response inhibition curves were shown for barramundi (Lates calcarifer), coho salmon (Oncorhynchus kisutch), Southern Bluefin tuna (Thunnus maccoyii), tilapia (Oreochromis mossambicus), and seabream (Pagrus auratus) IGF-I. The assay was then used to measure stress related changes in different aquacultured fish species. Salt water acclimated Atlantic salmon smolts (Salmo salar) bathed for 2 h in fresh water showed significantly lower IGF-I concentrations than control smolts two days after the bath (53.1 compared to 32.1 ng/ml), with levels of IGF-I also lower in smolts exhibiting stunted growth (stunts). Capture and confinement of wild tuna in sea-cages resulted in a significant decrease in IGF-I levels (28 ng/ml) when compared to tuna captured and sampled immediately (48 ng/ml), but had recovered to starting levels after 3 weeks (43 ng/ml). Handling and isolation in silver perch (Bidyanus bidyanus) led to a gradual decline in IGF-I over a 12 h period (36–19 ng/ml) but showed signs of recovery by 24 h (24 ng/ml) and had recovered fully 72 h after treatment (40 ng/ml). A similar trial in black bream (Acanthopagrus butcherii) showed comparable results with IGF-I levels gradually decreasing (40–26 ng/ml) over 24 h, results that were mirrored by cortisol concentrations which increased during this time (1–26 ng/ml). In the studies presented here changes in IGF-I levels were not observed for at least 3 h after exposure to the ... |
author2 |
School of Earth and Environmental Sciences : Ecology and Evolutionary Biology |
format |
Article in Journal/Newspaper |
author |
Dyer, Anthony R. Upton, Zee Stone, David Alan Joseph Thomas, Philip Mark Soole, Kathleen Lydia Higgs, Naomi Quinn, K. J. Carragher, John F. |
spellingShingle |
Dyer, Anthony R. Upton, Zee Stone, David Alan Joseph Thomas, Philip Mark Soole, Kathleen Lydia Higgs, Naomi Quinn, K. J. Carragher, John F. Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels |
author_facet |
Dyer, Anthony R. Upton, Zee Stone, David Alan Joseph Thomas, Philip Mark Soole, Kathleen Lydia Higgs, Naomi Quinn, K. J. Carragher, John F. |
author_sort |
Dyer, Anthony R. |
title |
Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels |
title_short |
Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels |
title_full |
Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels |
title_fullStr |
Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels |
title_full_unstemmed |
Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels |
title_sort |
development and validation of a radioimmunoassay for fish insulin-like growth factor i (igf-i) and the effect of aquaculture related stressors on circulating igf-i levels |
publisher |
Elsevier |
publishDate |
2004 |
url |
http://hdl.handle.net/2440/55192 https://doi.org/10.1016/j.ygcen.2003.10.002 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
General and Comparative Endocrinology, 2004; 135(3):268-275 0016-6480 http://hdl.handle.net/2440/55192 doi:10.1016/j.ygcen.2003.10.002 |
op_doi |
https://doi.org/10.1016/j.ygcen.2003.10.002 |
container_title |
General and Comparative Endocrinology |
container_volume |
135 |
container_issue |
3 |
container_start_page |
268 |
op_container_end_page |
275 |
_version_ |
1766363484161835008 |