Molecular characterization of the Rocky Mountain elk (Cervus elaphus nelsoni) PRNP putative promoter
© 2008 American Genetic Association Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) affecting deer (Odocoileus spp.), moose (Alces alces), and Rocky Mountain elk (Cervus elaphus nelsoni). Leucine homozygosity at elk PRNP codon 132 has been associated with reduced CWD...
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/46938 2023-05-15T13:13:25+02:00 Molecular characterization of the Rocky Mountain elk (Cervus elaphus nelsoni) PRNP putative promoter Seabury, C. Gill, C. Templeton, J. Dyar, J. Derr, J. Adelson, D. Owens, E. Davis, D. Kraemer, D. Womack, J. 2007 http://hdl.handle.net/2440/46938 https://doi.org/10.1093/jhered/esm091 en eng Oxford Univ Press Inc Journal of Heredity, 2007; 98(7):678-686 0022-1503 1465-7333 http://hdl.handle.net/2440/46938 doi:10.1093/jhered/esm091 Adelson, D. [0000-0003-2404-5636] http://jhered.oxfordjournals.org/cgi/content/abstract/esm091v1 Animals Deer Wasting Disease Chronic Prions DNA Sequence Analysis Base Sequence Gene Frequency Polymorphism Single Nucleotide Alleles Colorado Female Male Promoter Regions Genetic Journal article 2007 ftunivadelaidedl https://doi.org/10.1093/jhered/esm091 2023-02-05T19:25:23Z © 2008 American Genetic Association Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) affecting deer (Odocoileus spp.), moose (Alces alces), and Rocky Mountain elk (Cervus elaphus nelsoni). Leucine homozygosity at elk PRNP codon 132 has been associated with reduced CWD susceptibility. However, naturally acquired CWD has been detected in elk possessing the 132 Leu/Leu genotype. Recent human and bovine studies indicate that PRNP regulatory polymorphisms may also influence TSE occurrence. Therefore, we generated sequences for the elk PRNP putative promoter (2.2 kb), exon 1 (predicted; 54 bp), intron 1 (predicted; 193 bp), and exon 3 (771 bp). Promoter prediction analysis using CpGProD yielded a single elk PRNP promoter that was homologous to regions of known promoter activity in cow and sheep. Molecular interrogation of the elk PRNP putative promoter revealed 32 diallelic single-nucleotide polymorphisms (SNPs). No variation was detected within the predicted exon 1 or intron 1 sequences. Evaluation of elk PRNP exon 3 revealed 3 SNPs (63Y, 312R, 394WMet/Leu). Bayesian haplotype reconstruction resulted in 3 elk PRNP haplotypes, with complete linkage disequilibrium observed between all PRNP putative promoter SNPs and codon 132. The results of this study provide the initial genomic foundation for future comparative and haplotype-based elk PRNP studies. Article in Journal/Newspaper Alces alces The University of Adelaide: Digital Library Journal of Heredity 98 7 678 686 |
institution |
Open Polar |
collection |
The University of Adelaide: Digital Library |
op_collection_id |
ftunivadelaidedl |
language |
English |
topic |
Animals Deer Wasting Disease Chronic Prions DNA Sequence Analysis Base Sequence Gene Frequency Polymorphism Single Nucleotide Alleles Colorado Female Male Promoter Regions Genetic |
spellingShingle |
Animals Deer Wasting Disease Chronic Prions DNA Sequence Analysis Base Sequence Gene Frequency Polymorphism Single Nucleotide Alleles Colorado Female Male Promoter Regions Genetic Seabury, C. Gill, C. Templeton, J. Dyar, J. Derr, J. Adelson, D. Owens, E. Davis, D. Kraemer, D. Womack, J. Molecular characterization of the Rocky Mountain elk (Cervus elaphus nelsoni) PRNP putative promoter |
topic_facet |
Animals Deer Wasting Disease Chronic Prions DNA Sequence Analysis Base Sequence Gene Frequency Polymorphism Single Nucleotide Alleles Colorado Female Male Promoter Regions Genetic |
description |
© 2008 American Genetic Association Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) affecting deer (Odocoileus spp.), moose (Alces alces), and Rocky Mountain elk (Cervus elaphus nelsoni). Leucine homozygosity at elk PRNP codon 132 has been associated with reduced CWD susceptibility. However, naturally acquired CWD has been detected in elk possessing the 132 Leu/Leu genotype. Recent human and bovine studies indicate that PRNP regulatory polymorphisms may also influence TSE occurrence. Therefore, we generated sequences for the elk PRNP putative promoter (2.2 kb), exon 1 (predicted; 54 bp), intron 1 (predicted; 193 bp), and exon 3 (771 bp). Promoter prediction analysis using CpGProD yielded a single elk PRNP promoter that was homologous to regions of known promoter activity in cow and sheep. Molecular interrogation of the elk PRNP putative promoter revealed 32 diallelic single-nucleotide polymorphisms (SNPs). No variation was detected within the predicted exon 1 or intron 1 sequences. Evaluation of elk PRNP exon 3 revealed 3 SNPs (63Y, 312R, 394WMet/Leu). Bayesian haplotype reconstruction resulted in 3 elk PRNP haplotypes, with complete linkage disequilibrium observed between all PRNP putative promoter SNPs and codon 132. The results of this study provide the initial genomic foundation for future comparative and haplotype-based elk PRNP studies. |
format |
Article in Journal/Newspaper |
author |
Seabury, C. Gill, C. Templeton, J. Dyar, J. Derr, J. Adelson, D. Owens, E. Davis, D. Kraemer, D. Womack, J. |
author_facet |
Seabury, C. Gill, C. Templeton, J. Dyar, J. Derr, J. Adelson, D. Owens, E. Davis, D. Kraemer, D. Womack, J. |
author_sort |
Seabury, C. |
title |
Molecular characterization of the Rocky Mountain elk (Cervus elaphus nelsoni) PRNP putative promoter |
title_short |
Molecular characterization of the Rocky Mountain elk (Cervus elaphus nelsoni) PRNP putative promoter |
title_full |
Molecular characterization of the Rocky Mountain elk (Cervus elaphus nelsoni) PRNP putative promoter |
title_fullStr |
Molecular characterization of the Rocky Mountain elk (Cervus elaphus nelsoni) PRNP putative promoter |
title_full_unstemmed |
Molecular characterization of the Rocky Mountain elk (Cervus elaphus nelsoni) PRNP putative promoter |
title_sort |
molecular characterization of the rocky mountain elk (cervus elaphus nelsoni) prnp putative promoter |
publisher |
Oxford Univ Press Inc |
publishDate |
2007 |
url |
http://hdl.handle.net/2440/46938 https://doi.org/10.1093/jhered/esm091 |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
http://jhered.oxfordjournals.org/cgi/content/abstract/esm091v1 |
op_relation |
Journal of Heredity, 2007; 98(7):678-686 0022-1503 1465-7333 http://hdl.handle.net/2440/46938 doi:10.1093/jhered/esm091 Adelson, D. [0000-0003-2404-5636] |
op_doi |
https://doi.org/10.1093/jhered/esm091 |
container_title |
Journal of Heredity |
container_volume |
98 |
container_issue |
7 |
container_start_page |
678 |
op_container_end_page |
686 |
_version_ |
1766258288897294336 |