Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community.
Different organs of a host represent distinct microenvironments resulting in the establishment of multiple discrete bacterial communities within a host. These discrete bacterial communities can also vary according to geographical location. For the Pacific oyster, Crassostrea gigas, the factors gover...
Published in: | FEMS Microbiology Ecology |
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Online Access: | https://hdl.handle.net/2440/139298 https://doi.org/10.1093/femsec/fiaa054 |
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/139298 2023-12-17T10:29:13+01:00 Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community. King, W.L. Siboni, N. Kahlke, T. Dove, M. O'Connor, W. Mahbub, K.R. Jenkins, C. Seymour, J.R. Labbate, M. 2020 https://hdl.handle.net/2440/139298 https://doi.org/10.1093/femsec/fiaa054 en eng Oxford University Press (OUP) http://purl.org/au-research/grants/arc/LP160101785 FEMS Microbiology Ecology, 2020; 96(5):fiaa054-fiaa054 0168-6496 1574-6941 https://hdl.handle.net/2440/139298 doi:10.1093/femsec/fiaa054 Mahbub, K.R. [0000-0001-7086-9965] © FEMS 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com http://dx.doi.org/10.1093/femsec/fiaa054 Crassostrea microbiome microenvironments core microbiome bivalve invertebrate microbiota Gills Animals Bacteria RNA Ribosomal 16S Microbiota Journal article 2020 ftunivadelaidedl https://doi.org/10.1093/femsec/fiaa054 2023-11-20T23:34:51Z Different organs of a host represent distinct microenvironments resulting in the establishment of multiple discrete bacterial communities within a host. These discrete bacterial communities can also vary according to geographical location. For the Pacific oyster, Crassostrea gigas, the factors governing bacterial diversity and abundance of different oyster microenvironments are poorly understood. In this study, the factors shaping bacterial abundance, diversity and composition associated with the C. gigas mantle, gill, adductor muscle and digestive gland were characterised using 16S (V3-V4) rRNA amplicon sequencing across six discrete estuaries. Both location and tissue-type, with tissue-type being the stronger determinant, were factors driving bacterial community composition. Bacterial communities from wave-dominated estuaries had similar compositions and higher bacterial abundance despite being geographically distant from one another, possibly indicating that functional estuarine morphology characteristics are a factor shaping the oyster bacterial community. Despite the bacterial community heterogeneity, examinations of the core bacterial community identified Spirochaetaceae bacteria as conserved across all sites and samples. Whereas members of the Vulcaniibacterium, Spirochaetaceae and Margulisbacteria, and Polynucleobacter were regionally conserved members of the digestive gland, gill and mantle bacterial communities, respectively. This indicates that baseline bacterial community profiles for specific locations are necessary when investigating bacterial communities in oyster health. William L King, Nachshon Siboni, Tim Kahlke, Michael Dove, Wayne O'Connor, Khandaker Rayhan Mahbub, Cheryl Jenkins, Justin R Seymour, and Maurizio Labbate Article in Journal/Newspaper Crassostrea gigas Pacific oyster The University of Adelaide: Digital Library O'Connor ENVELOPE(-58.383,-58.383,-62.067,-62.067) Pacific Seymour ENVELOPE(-56.767,-56.767,-64.283,-64.283) FEMS Microbiology Ecology 96 5 |
institution |
Open Polar |
collection |
The University of Adelaide: Digital Library |
op_collection_id |
ftunivadelaidedl |
language |
English |
topic |
Crassostrea microbiome microenvironments core microbiome bivalve invertebrate microbiota Gills Animals Bacteria RNA Ribosomal 16S Microbiota |
spellingShingle |
Crassostrea microbiome microenvironments core microbiome bivalve invertebrate microbiota Gills Animals Bacteria RNA Ribosomal 16S Microbiota King, W.L. Siboni, N. Kahlke, T. Dove, M. O'Connor, W. Mahbub, K.R. Jenkins, C. Seymour, J.R. Labbate, M. Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community. |
topic_facet |
Crassostrea microbiome microenvironments core microbiome bivalve invertebrate microbiota Gills Animals Bacteria RNA Ribosomal 16S Microbiota |
description |
Different organs of a host represent distinct microenvironments resulting in the establishment of multiple discrete bacterial communities within a host. These discrete bacterial communities can also vary according to geographical location. For the Pacific oyster, Crassostrea gigas, the factors governing bacterial diversity and abundance of different oyster microenvironments are poorly understood. In this study, the factors shaping bacterial abundance, diversity and composition associated with the C. gigas mantle, gill, adductor muscle and digestive gland were characterised using 16S (V3-V4) rRNA amplicon sequencing across six discrete estuaries. Both location and tissue-type, with tissue-type being the stronger determinant, were factors driving bacterial community composition. Bacterial communities from wave-dominated estuaries had similar compositions and higher bacterial abundance despite being geographically distant from one another, possibly indicating that functional estuarine morphology characteristics are a factor shaping the oyster bacterial community. Despite the bacterial community heterogeneity, examinations of the core bacterial community identified Spirochaetaceae bacteria as conserved across all sites and samples. Whereas members of the Vulcaniibacterium, Spirochaetaceae and Margulisbacteria, and Polynucleobacter were regionally conserved members of the digestive gland, gill and mantle bacterial communities, respectively. This indicates that baseline bacterial community profiles for specific locations are necessary when investigating bacterial communities in oyster health. William L King, Nachshon Siboni, Tim Kahlke, Michael Dove, Wayne O'Connor, Khandaker Rayhan Mahbub, Cheryl Jenkins, Justin R Seymour, and Maurizio Labbate |
format |
Article in Journal/Newspaper |
author |
King, W.L. Siboni, N. Kahlke, T. Dove, M. O'Connor, W. Mahbub, K.R. Jenkins, C. Seymour, J.R. Labbate, M. |
author_facet |
King, W.L. Siboni, N. Kahlke, T. Dove, M. O'Connor, W. Mahbub, K.R. Jenkins, C. Seymour, J.R. Labbate, M. |
author_sort |
King, W.L. |
title |
Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community. |
title_short |
Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community. |
title_full |
Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community. |
title_fullStr |
Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community. |
title_full_unstemmed |
Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community. |
title_sort |
regional and oyster microenvironmental scale heterogeneity in the pacific oyster bacterial community. |
publisher |
Oxford University Press (OUP) |
publishDate |
2020 |
url |
https://hdl.handle.net/2440/139298 https://doi.org/10.1093/femsec/fiaa054 |
long_lat |
ENVELOPE(-58.383,-58.383,-62.067,-62.067) ENVELOPE(-56.767,-56.767,-64.283,-64.283) |
geographic |
O'Connor Pacific Seymour |
geographic_facet |
O'Connor Pacific Seymour |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
http://dx.doi.org/10.1093/femsec/fiaa054 |
op_relation |
http://purl.org/au-research/grants/arc/LP160101785 FEMS Microbiology Ecology, 2020; 96(5):fiaa054-fiaa054 0168-6496 1574-6941 https://hdl.handle.net/2440/139298 doi:10.1093/femsec/fiaa054 Mahbub, K.R. [0000-0001-7086-9965] |
op_rights |
© FEMS 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com |
op_doi |
https://doi.org/10.1093/femsec/fiaa054 |
container_title |
FEMS Microbiology Ecology |
container_volume |
96 |
container_issue |
5 |
_version_ |
1785581549860683776 |