Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques
The accurate observation of physiological changes on in vivo samples of important animal species such as Euphausia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized...
Published in: | Marine and Freshwater Behaviour and Physiology |
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Language: | English |
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2015
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Online Access: | http://hdl.handle.net/2440/130419 https://doi.org/10.1080/10236244.2015.1073455 |
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/130419 2023-12-17T10:20:56+01:00 Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques Cox, M. Kawaguchi, S. King, R. Dholakia, K. Brown, C.T.A. 2015 http://hdl.handle.net/2440/130419 https://doi.org/10.1080/10236244.2015.1073455 en eng Taylor & Francis http://purl.org/au-research/grants/arc/FS110200057 Marine and Freshwater Behaviour and Physiology, 2015; 48(6):455-466 1023-6244 1029-0362 http://hdl.handle.net/2440/130419 doi:10.1080/10236244.2015.1073455 Dholakia, K. [0000-0001-6534-9009] © 2015 Taylor & Francis http://dx.doi.org/10.1080/10236244.2015.1073455 krill trap live animal morphological observations optical coherence tomography optical imaging Journal article 2015 ftunivadelaidedl https://doi.org/10.1080/10236244.2015.1073455 2023-11-20T23:30:21Z The accurate observation of physiological changes on in vivo samples of important animal species such as Euphausia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: optical coherence tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animals in vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development. Martin Cox, So Kawaguchi, Robert King, Kishan Dholakia and Christian T.A. Brown Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba The University of Adelaide: Digital Library Antarctic Marine and Freshwater Behaviour and Physiology 48 6 455 466 |
institution |
Open Polar |
collection |
The University of Adelaide: Digital Library |
op_collection_id |
ftunivadelaidedl |
language |
English |
topic |
krill trap live animal morphological observations optical coherence tomography optical imaging |
spellingShingle |
krill trap live animal morphological observations optical coherence tomography optical imaging Cox, M. Kawaguchi, S. King, R. Dholakia, K. Brown, C.T.A. Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
topic_facet |
krill trap live animal morphological observations optical coherence tomography optical imaging |
description |
The accurate observation of physiological changes on in vivo samples of important animal species such as Euphausia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: optical coherence tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animals in vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development. Martin Cox, So Kawaguchi, Robert King, Kishan Dholakia and Christian T.A. Brown |
format |
Article in Journal/Newspaper |
author |
Cox, M. Kawaguchi, S. King, R. Dholakia, K. Brown, C.T.A. |
author_facet |
Cox, M. Kawaguchi, S. King, R. Dholakia, K. Brown, C.T.A. |
author_sort |
Cox, M. |
title |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_short |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_full |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_fullStr |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_full_unstemmed |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_sort |
internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (oct) imaging techniques |
publisher |
Taylor & Francis |
publishDate |
2015 |
url |
http://hdl.handle.net/2440/130419 https://doi.org/10.1080/10236244.2015.1073455 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_source |
http://dx.doi.org/10.1080/10236244.2015.1073455 |
op_relation |
http://purl.org/au-research/grants/arc/FS110200057 Marine and Freshwater Behaviour and Physiology, 2015; 48(6):455-466 1023-6244 1029-0362 http://hdl.handle.net/2440/130419 doi:10.1080/10236244.2015.1073455 Dholakia, K. [0000-0001-6534-9009] |
op_rights |
© 2015 Taylor & Francis |
op_doi |
https://doi.org/10.1080/10236244.2015.1073455 |
container_title |
Marine and Freshwater Behaviour and Physiology |
container_volume |
48 |
container_issue |
6 |
container_start_page |
455 |
op_container_end_page |
466 |
_version_ |
1785527681635319808 |