A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections
Cathepsin Z (CTSZ) is lysosomal cysteine protease of the papain superfamily. It participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. Hence, CTSZ is als...
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Online Access: | http://hdl.handle.net/2440/120797 https://doi.org/10.1016/j.gene.2017.07.007 |
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/120797 2023-12-17T10:29:16+01:00 A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections Godahewa, G.I. Perera, N.C.N. Lee, S. Kim, M.J. Lee, J. 2017 http://hdl.handle.net/2440/120797 https://doi.org/10.1016/j.gene.2017.07.007 en eng Elsevier Gene, 2017; 627:500-507 0378-1119 1879-0038 http://hdl.handle.net/2440/120797 doi:10.1016/j.gene.2017.07.007 Godahewa, G.I. [0000-0002-7614-1040] © 2017 Elsevier B.V. All rights reserved. http://dx.doi.org/10.1016/j.gene.2017.07.007 Cathepsin Z Disk abalone Abalone early development Genomic organization Immune responsive expression Journal article 2017 ftunivadelaidedl https://doi.org/10.1016/j.gene.2017.07.007 2023-11-20T23:25:44Z Cathepsin Z (CTSZ) is lysosomal cysteine protease of the papain superfamily. It participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. Hence, CTSZ is also acknowledged as an acute-phase protein in host immunity. In this study, we sought to identify the CTSZ homolog from disk abalone (AbCTSZ) and characterize it at the molecular, genomic, and transcriptional levels. AbCTSZ encodes a protein with 318 amino acids and a molecular mass of 36kDa. The structure of AbCTSZ reveals amino acid sequences that are characteristic of the signal sequence, pro-peptide, peptidase-C1 papain family cysteine protease domain, mini-loop, HIP motif, N-linked glycosylation sites, active sites, and conserved Cys residues. A pairwise comparison revealed that AbCTSZ shared the highest amino acid homology with its molluscan counterpart from Crassostrea gigas. A multiple alignment analysis revealed the conservation of functionally crucial elements of AbCTSZ, and a phylogenetic study further confirmed a proximal evolutionary relationship with its invertebrate counterparts. Further, an analysis of AbCTSZ genomic structure revealed seven exons separated by six introns, which differs from that of its vertebrate counterparts. Quantitative real time PCR (qPCR) detected the transcripts of AbCTSZ in early developmental stages and in eight different tissues. Higher levels of AbCTSZ transcripts were found in trochophore, gill, and hemocytes, highlighting its importance in the early development and immunity of disk abalone. In addition, we found that viable bacteria (Vibrio parahaemolyticus and Listeria monocytogenes) and bacterial lipopolysaccharides significantly modulated AbCTSZ transcription. Collectively, these lines of evidences suggest that AbCTSZ plays an indispensable role in the innate immunity of disk abalone. G. I. Godahewa, N. C. N. Perera, Sukkyoung Lee, Myoung-Jin Kim, Jehee Lee Article in Journal/Newspaper Crassostrea gigas The University of Adelaide: Digital Library Gene 627 500 507 |
institution |
Open Polar |
collection |
The University of Adelaide: Digital Library |
op_collection_id |
ftunivadelaidedl |
language |
English |
topic |
Cathepsin Z Disk abalone Abalone early development Genomic organization Immune responsive expression |
spellingShingle |
Cathepsin Z Disk abalone Abalone early development Genomic organization Immune responsive expression Godahewa, G.I. Perera, N.C.N. Lee, S. Kim, M.J. Lee, J. A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections |
topic_facet |
Cathepsin Z Disk abalone Abalone early development Genomic organization Immune responsive expression |
description |
Cathepsin Z (CTSZ) is lysosomal cysteine protease of the papain superfamily. It participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. Hence, CTSZ is also acknowledged as an acute-phase protein in host immunity. In this study, we sought to identify the CTSZ homolog from disk abalone (AbCTSZ) and characterize it at the molecular, genomic, and transcriptional levels. AbCTSZ encodes a protein with 318 amino acids and a molecular mass of 36kDa. The structure of AbCTSZ reveals amino acid sequences that are characteristic of the signal sequence, pro-peptide, peptidase-C1 papain family cysteine protease domain, mini-loop, HIP motif, N-linked glycosylation sites, active sites, and conserved Cys residues. A pairwise comparison revealed that AbCTSZ shared the highest amino acid homology with its molluscan counterpart from Crassostrea gigas. A multiple alignment analysis revealed the conservation of functionally crucial elements of AbCTSZ, and a phylogenetic study further confirmed a proximal evolutionary relationship with its invertebrate counterparts. Further, an analysis of AbCTSZ genomic structure revealed seven exons separated by six introns, which differs from that of its vertebrate counterparts. Quantitative real time PCR (qPCR) detected the transcripts of AbCTSZ in early developmental stages and in eight different tissues. Higher levels of AbCTSZ transcripts were found in trochophore, gill, and hemocytes, highlighting its importance in the early development and immunity of disk abalone. In addition, we found that viable bacteria (Vibrio parahaemolyticus and Listeria monocytogenes) and bacterial lipopolysaccharides significantly modulated AbCTSZ transcription. Collectively, these lines of evidences suggest that AbCTSZ plays an indispensable role in the innate immunity of disk abalone. G. I. Godahewa, N. C. N. Perera, Sukkyoung Lee, Myoung-Jin Kim, Jehee Lee |
format |
Article in Journal/Newspaper |
author |
Godahewa, G.I. Perera, N.C.N. Lee, S. Kim, M.J. Lee, J. |
author_facet |
Godahewa, G.I. Perera, N.C.N. Lee, S. Kim, M.J. Lee, J. |
author_sort |
Godahewa, G.I. |
title |
A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections |
title_short |
A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections |
title_full |
A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections |
title_fullStr |
A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections |
title_full_unstemmed |
A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections |
title_sort |
cysteine protease (cathepsin z) from disk abalone, haliotis discus discus: genomic characterization and transcriptional profiling during bacterial infections |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://hdl.handle.net/2440/120797 https://doi.org/10.1016/j.gene.2017.07.007 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
http://dx.doi.org/10.1016/j.gene.2017.07.007 |
op_relation |
Gene, 2017; 627:500-507 0378-1119 1879-0038 http://hdl.handle.net/2440/120797 doi:10.1016/j.gene.2017.07.007 Godahewa, G.I. [0000-0002-7614-1040] |
op_rights |
© 2017 Elsevier B.V. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.gene.2017.07.007 |
container_title |
Gene |
container_volume |
627 |
container_start_page |
500 |
op_container_end_page |
507 |
_version_ |
1785581602964766720 |