Novel mitochondrial DNA primers for identification of population trends in longnose suckers (Catostomus catostomus) and multispecies identification
The understudied longnose sucker has received no attention by the International Union for Conservation of Nature wherein almost 55 % of fish in this family have been classified as threatened, endangered, extinct, or experiencing population declines. Understanding population trends of the ecologicall...
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Online Access: | http://hdl.handle.net/2440/112491 https://doi.org/10.1007/s12686-013-0111-6 |
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/112491 2023-12-17T10:28:47+01:00 Novel mitochondrial DNA primers for identification of population trends in longnose suckers (Catostomus catostomus) and multispecies identification Langille, B. Perry, R. Keefe, D. Marshall, H. 2014 http://hdl.handle.net/2440/112491 https://doi.org/10.1007/s12686-013-0111-6 en eng Springer Conservation Genetics Resources, 2014; 6(2):279-281 1877-7252 1877-7260 http://hdl.handle.net/2440/112491 doi:10.1007/s12686-013-0111-6 © Springer Science+Business Media Dordrecht 2013 http://dx.doi.org/10.1007/s12686-013-0111-6 Longnose sucker IUCN hybridization cross-species mitochondrial DNA landscape studies Journal article 2014 ftunivadelaidedl https://doi.org/10.1007/s12686-013-0111-6 2023-11-20T23:29:23Z The understudied longnose sucker has received no attention by the International Union for Conservation of Nature wherein almost 55 % of fish in this family have been classified as threatened, endangered, extinct, or experiencing population declines. Understanding population trends of the ecologically diverse longnose sucker is critical for preservation of this unique species, as it hybridizes readily with congenerics. Here we describe three novel pairs of mitochondrial DNA (mtDNA) primers with which we amplified genes from four species: longnose and white suckers, round whitefish, and slimy sculpin. Nucleotide substitutions ranged from 10 to 111 between individual longnose suckers and among various species. Hybridization may be occurring, as a morphologically identified longnose sucker contained white sucker mtDNA. Species level identification using these primers within a larger scope of landscape studies is possible as these species belong to three orders, showing the utility of these primers on a variety of taxa. Barbara L. Langille, Robert Perry, Donald Keefe, H. Dawn Marshall Article in Journal/Newspaper Catostomus catostomus Longnose sucker Slimy sculpin The University of Adelaide: Digital Library Conservation Genetics Resources 6 2 279 281 |
institution |
Open Polar |
collection |
The University of Adelaide: Digital Library |
op_collection_id |
ftunivadelaidedl |
language |
English |
topic |
Longnose sucker IUCN hybridization cross-species mitochondrial DNA landscape studies |
spellingShingle |
Longnose sucker IUCN hybridization cross-species mitochondrial DNA landscape studies Langille, B. Perry, R. Keefe, D. Marshall, H. Novel mitochondrial DNA primers for identification of population trends in longnose suckers (Catostomus catostomus) and multispecies identification |
topic_facet |
Longnose sucker IUCN hybridization cross-species mitochondrial DNA landscape studies |
description |
The understudied longnose sucker has received no attention by the International Union for Conservation of Nature wherein almost 55 % of fish in this family have been classified as threatened, endangered, extinct, or experiencing population declines. Understanding population trends of the ecologically diverse longnose sucker is critical for preservation of this unique species, as it hybridizes readily with congenerics. Here we describe three novel pairs of mitochondrial DNA (mtDNA) primers with which we amplified genes from four species: longnose and white suckers, round whitefish, and slimy sculpin. Nucleotide substitutions ranged from 10 to 111 between individual longnose suckers and among various species. Hybridization may be occurring, as a morphologically identified longnose sucker contained white sucker mtDNA. Species level identification using these primers within a larger scope of landscape studies is possible as these species belong to three orders, showing the utility of these primers on a variety of taxa. Barbara L. Langille, Robert Perry, Donald Keefe, H. Dawn Marshall |
format |
Article in Journal/Newspaper |
author |
Langille, B. Perry, R. Keefe, D. Marshall, H. |
author_facet |
Langille, B. Perry, R. Keefe, D. Marshall, H. |
author_sort |
Langille, B. |
title |
Novel mitochondrial DNA primers for identification of population trends in longnose suckers (Catostomus catostomus) and multispecies identification |
title_short |
Novel mitochondrial DNA primers for identification of population trends in longnose suckers (Catostomus catostomus) and multispecies identification |
title_full |
Novel mitochondrial DNA primers for identification of population trends in longnose suckers (Catostomus catostomus) and multispecies identification |
title_fullStr |
Novel mitochondrial DNA primers for identification of population trends in longnose suckers (Catostomus catostomus) and multispecies identification |
title_full_unstemmed |
Novel mitochondrial DNA primers for identification of population trends in longnose suckers (Catostomus catostomus) and multispecies identification |
title_sort |
novel mitochondrial dna primers for identification of population trends in longnose suckers (catostomus catostomus) and multispecies identification |
publisher |
Springer |
publishDate |
2014 |
url |
http://hdl.handle.net/2440/112491 https://doi.org/10.1007/s12686-013-0111-6 |
genre |
Catostomus catostomus Longnose sucker Slimy sculpin |
genre_facet |
Catostomus catostomus Longnose sucker Slimy sculpin |
op_source |
http://dx.doi.org/10.1007/s12686-013-0111-6 |
op_relation |
Conservation Genetics Resources, 2014; 6(2):279-281 1877-7252 1877-7260 http://hdl.handle.net/2440/112491 doi:10.1007/s12686-013-0111-6 |
op_rights |
© Springer Science+Business Media Dordrecht 2013 |
op_doi |
https://doi.org/10.1007/s12686-013-0111-6 |
container_title |
Conservation Genetics Resources |
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6 |
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2 |
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279 |
op_container_end_page |
281 |
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1785580965269078016 |