Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric G-protein
Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity (PTI) and disease resistance responses to different types of pathogens. It also plays a role in plant cell wall integrity as mutants impaired in the Gβ- (agb1-2) or Gγ-subunits have an alt...
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Online Access: | http://hdl.handle.net/2440/110588 https://doi.org/10.1111/tpj.13660 |
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ftunivadelaidedl:oai:digital.library.adelaide.edu.au:2440/110588 2023-12-24T10:16:27+01:00 Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric G-protein Escudero, V. Jordá, L. Sopeña-Torres, S. Mélida, H. Miedes, E. Muñoz-Barrios, A. Swami, S. Alexander, D. McKee, L. Sánchez-Vallet, A. Bulone, V. Jones, A. Molina, A. 2017 application/pdf http://hdl.handle.net/2440/110588 https://doi.org/10.1111/tpj.13660 en eng Wiley ARC The Plant Journal, 2017; 92(3):386-399 0960-7412 1365-313X http://hdl.handle.net/2440/110588 doi:10.1111/tpj.13660 Bulone, V. [0000-0002-9742-4701] [0000-0003-2809-4160] © 2017 The Authors The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. http://dx.doi.org/10.1111/tpj.13660 Heterotrimeric G-protein AGB1 agb1-2 plant cell wall xylan necrotrophic fungi immunity pathogen-associated molecular pattern Plectosphaerella cucumerina Arabidopsis thaliana Journal article 2017 ftunivadelaidedl https://doi.org/10.1111/tpj.13660 2023-11-27T23:25:15Z Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity (PTI) and disease resistance responses to different types of pathogens. It also plays a role in plant cell wall integrity as mutants impaired in the Gβ- (agb1-2) or Gγ-subunits have an altered wall composition compared with wild-type plants. Here we performed a mutant screen to identify suppressors of agb1-2 (sgb) that restore susceptibility to pathogens to wild-type levels. Out of the four sgb mutants (sgb10-sgb13) identified, sgb11 is a new mutant allele of ESKIMO1 (ESK1), which encodes a plant-specific polysaccharide O-acetyltransferase involved in xylan acetylation. Null alleles (sgb11/esk1-7) of ESK1 restore to wild-type levels the enhanced susceptibility of agb1-2 to the necrotrophic fungus Plectosphaerella cucumerina BMM (PcBMM), but not to the bacterium Pseudomonas syringae pv. tomato DC3000 or to the oomycete Hyaloperonospora arabidopsidis. The enhanced resistance to PcBMM of the agb1-2 esk1-7 double mutant was not the result of the re-activation of deficient PTI responses in agb1-2. Alteration of cell wall xylan acetylation caused by ESK1 impairment was accompanied by an enhanced accumulation of abscisic acid, the constitutive expression of genes encoding antibiotic peptides and enzymes involved in the biosynthesis of tryptophan-derived metabolites, and the accumulation of disease resistance-related secondary metabolites and different osmolites. These esk1-mediated responses counterbalance the defective PTI and PcBMM susceptibility of agb1-2 plants, and explain the enhanced drought resistance of esk1 plants. These results suggest that a deficient PTI-mediated resistance is partially compensated by the activation of specific cell-wall-triggered immune responses. Viviana Escudero, Lucía Jordá, Sara Sopeña-Torres, Hugo Mélida, Eva Miedes, Antonio Muñoz-Barrios, Sanjay Swami, Danny Alexander, Lauren S. McKee, Andrea Sánchez-Vallet, Vincent Bulone, Alan M. Jones, and Antonio Molina Article in Journal/Newspaper eskimo* The University of Adelaide: Digital Library Molina ENVELOPE(-62.017,-62.017,-64.017,-64.017) Barrios ENVELOPE(-57.950,-57.950,-63.324,-63.324) Muñoz ENVELOPE(-64.133,-64.133,-66.750,-66.750) Lucía ENVELOPE(-57.033,-57.033,-63.450,-63.450) Escudero ENVELOPE(-64.817,-64.817,-67.317,-67.317) The Plant Journal 92 3 386 399 |
institution |
Open Polar |
collection |
The University of Adelaide: Digital Library |
op_collection_id |
ftunivadelaidedl |
language |
English |
topic |
Heterotrimeric G-protein AGB1 agb1-2 plant cell wall xylan necrotrophic fungi immunity pathogen-associated molecular pattern Plectosphaerella cucumerina Arabidopsis thaliana |
spellingShingle |
Heterotrimeric G-protein AGB1 agb1-2 plant cell wall xylan necrotrophic fungi immunity pathogen-associated molecular pattern Plectosphaerella cucumerina Arabidopsis thaliana Escudero, V. Jordá, L. Sopeña-Torres, S. Mélida, H. Miedes, E. Muñoz-Barrios, A. Swami, S. Alexander, D. McKee, L. Sánchez-Vallet, A. Bulone, V. Jones, A. Molina, A. Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric G-protein |
topic_facet |
Heterotrimeric G-protein AGB1 agb1-2 plant cell wall xylan necrotrophic fungi immunity pathogen-associated molecular pattern Plectosphaerella cucumerina Arabidopsis thaliana |
description |
Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity (PTI) and disease resistance responses to different types of pathogens. It also plays a role in plant cell wall integrity as mutants impaired in the Gβ- (agb1-2) or Gγ-subunits have an altered wall composition compared with wild-type plants. Here we performed a mutant screen to identify suppressors of agb1-2 (sgb) that restore susceptibility to pathogens to wild-type levels. Out of the four sgb mutants (sgb10-sgb13) identified, sgb11 is a new mutant allele of ESKIMO1 (ESK1), which encodes a plant-specific polysaccharide O-acetyltransferase involved in xylan acetylation. Null alleles (sgb11/esk1-7) of ESK1 restore to wild-type levels the enhanced susceptibility of agb1-2 to the necrotrophic fungus Plectosphaerella cucumerina BMM (PcBMM), but not to the bacterium Pseudomonas syringae pv. tomato DC3000 or to the oomycete Hyaloperonospora arabidopsidis. The enhanced resistance to PcBMM of the agb1-2 esk1-7 double mutant was not the result of the re-activation of deficient PTI responses in agb1-2. Alteration of cell wall xylan acetylation caused by ESK1 impairment was accompanied by an enhanced accumulation of abscisic acid, the constitutive expression of genes encoding antibiotic peptides and enzymes involved in the biosynthesis of tryptophan-derived metabolites, and the accumulation of disease resistance-related secondary metabolites and different osmolites. These esk1-mediated responses counterbalance the defective PTI and PcBMM susceptibility of agb1-2 plants, and explain the enhanced drought resistance of esk1 plants. These results suggest that a deficient PTI-mediated resistance is partially compensated by the activation of specific cell-wall-triggered immune responses. Viviana Escudero, Lucía Jordá, Sara Sopeña-Torres, Hugo Mélida, Eva Miedes, Antonio Muñoz-Barrios, Sanjay Swami, Danny Alexander, Lauren S. McKee, Andrea Sánchez-Vallet, Vincent Bulone, Alan M. Jones, and Antonio Molina |
format |
Article in Journal/Newspaper |
author |
Escudero, V. Jordá, L. Sopeña-Torres, S. Mélida, H. Miedes, E. Muñoz-Barrios, A. Swami, S. Alexander, D. McKee, L. Sánchez-Vallet, A. Bulone, V. Jones, A. Molina, A. |
author_facet |
Escudero, V. Jordá, L. Sopeña-Torres, S. Mélida, H. Miedes, E. Muñoz-Barrios, A. Swami, S. Alexander, D. McKee, L. Sánchez-Vallet, A. Bulone, V. Jones, A. Molina, A. |
author_sort |
Escudero, V. |
title |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric G-protein |
title_short |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric G-protein |
title_full |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric G-protein |
title_fullStr |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric G-protein |
title_full_unstemmed |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric G-protein |
title_sort |
alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric g-protein |
publisher |
Wiley |
publishDate |
2017 |
url |
http://hdl.handle.net/2440/110588 https://doi.org/10.1111/tpj.13660 |
long_lat |
ENVELOPE(-62.017,-62.017,-64.017,-64.017) ENVELOPE(-57.950,-57.950,-63.324,-63.324) ENVELOPE(-64.133,-64.133,-66.750,-66.750) ENVELOPE(-57.033,-57.033,-63.450,-63.450) ENVELOPE(-64.817,-64.817,-67.317,-67.317) |
geographic |
Molina Barrios Muñoz Lucía Escudero |
geographic_facet |
Molina Barrios Muñoz Lucía Escudero |
genre |
eskimo* |
genre_facet |
eskimo* |
op_source |
http://dx.doi.org/10.1111/tpj.13660 |
op_relation |
ARC The Plant Journal, 2017; 92(3):386-399 0960-7412 1365-313X http://hdl.handle.net/2440/110588 doi:10.1111/tpj.13660 Bulone, V. [0000-0002-9742-4701] [0000-0003-2809-4160] |
op_rights |
© 2017 The Authors The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_doi |
https://doi.org/10.1111/tpj.13660 |
container_title |
The Plant Journal |
container_volume |
92 |
container_issue |
3 |
container_start_page |
386 |
op_container_end_page |
399 |
_version_ |
1786204011120033792 |