Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics

• Published in: Frontiers in Immunology
• Main Authors: West, Alex C., Mizoro, Yasutaka, Wood, Shona H., Ince, Louise M., Iversen, Marianne, Jørgensen, Even H., Nome, Torfinn, Sandve, Simen Rød, Martin, Samuel A. M., Loudon, Andrew S. I., Hazlerigg, David G.
• Other Authors: University of Aberdeen.Biological Sciences, University of Aberdeen.Marine Alliance for Science and Technology for Scotland (MASTS), University of Aberdeen.Scottish Fish Immunology Research Centre
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2021
• Subjects: SDG 14 - Life Below Water; Atlantic salmon (Salmo salar); smoltification; photoperiod; immune cells; Gill; single nuclei RNA sequencing; QH301 Biology; Supplementary Information; QH301; Atlantic salmon; Salmo salar; Tromsø
• Online Access: https://hdl.handle.net/2164/16374; https://doi.org/10.3389/fimmu.2021.669889

ACKNOWLEDGMENTS The authors thank all of the animal staff at Kårvik havbruksstasjonen for their expert care of the research animals, and the University of Manchester Genomics Technology core facility (UK) for performing chromium 10x library preparation for snRNAseq. We also thanks the reviewers for their constructive comments on the original manuscript FUNDING AW is supported by the Tromsø forskningsstiftelse (TFS) grant awarded to DH (TFS2016DH). The Sentinel North Transdisciplinary Research Program Université Laval and UiT awarded to DH supports this work. SW is supported a grant from the Tromsø forskningsstiftelse (TFS) starter grant TFS2016SW. Experimental costs were covered by HFSP grant “Evolution of seasonal timers” RGP0030/2015 awarded to AL and DH. Storage resources were provided by the Norwegian National Infrastructure for Research Data (NIRD, project NS9055K). Peer reviewed