Transcriptomic Profiling of the Adaptive and Innate Immune Responses of Atlantic Salmon to Renibacterium salmoninarum Infection
Indexación: Scopus. Bacterial Kidney Disease (BKD), which is caused by a Gram-positive, intracellular bacterial pathogen (Renibacterium salmoninarum), affects salmonids including Atlantic salmon (Salmo salar). However, the transcriptome response of Atlantic salmon to BKD remained unknown before the...
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ftunivabello:oai:repositorio.unab.cl:ria/19400 2023-05-15T15:30:15+02:00 Transcriptomic Profiling of the Adaptive and Innate Immune Responses of Atlantic Salmon to Renibacterium salmoninarum Infection Eslamloo, K. Caballero-Solares, A. Inkpen, S.M. Emam, M. Kumar, S. Bouniot, C. Avendaño-Herrera, R. Jakob, E. Rise, M.L. 2020-10 application/pdf http://repositorio.unab.cl/xmlui/handle/ria/19400 https://doi.org/10.3389/fimmu.2020.567838 en eng Frontiers Media S.A. Frontiers in ImmunologyOpen AccessVolume 1128 October 2020 Article number 567838 1664-3224 http://repositorio.unab.cl/xmlui/handle/ria/19400 doi:10.3389/fimmu.2020.567838 https://creativecommons.org/licenses/by/4.0/ Attribution 4.0 International (CC BY 4.0) CC-BY responses immune response infection Article 2020 ftunivabello https://doi.org/10.3389/fimmu.2020.567838 2022-12-27T16:54:04Z Indexación: Scopus. Bacterial Kidney Disease (BKD), which is caused by a Gram-positive, intracellular bacterial pathogen (Renibacterium salmoninarum), affects salmonids including Atlantic salmon (Salmo salar). However, the transcriptome response of Atlantic salmon to BKD remained unknown before the current study. We used a 44K salmonid microarray platform to characterise the global gene expression response of Atlantic salmon to BKD. Fish (~54 g) were injected with a dose of R. salmoninarum (H-2 strain, 2 × 108 CFU per fish) or sterile medium (control), and then head kidney samples were collected at 13 days post-infection/injection (dpi). Firstly, infection levels of individuals were determined through quantifying the R. salmoninarum level by RNA-based TaqMan qPCR assays. Thereafter, based on the qPCR results for infection level, fish (n = 5) that showed no (control), higher (H-BKD), or lower (L-BKD) infection level at 13 dpi were subjected to microarray analyses. We identified 6,766 and 7,729 differentially expressed probes in the H-BKD and L-BKD groups, respectively. There were 357 probes responsive to the infection level (H-BKD vs. L-BKD). Several adaptive and innate immune processes were dysregulated in R. salmoninarum-infected Atlantic salmon. Adaptive immune pathways associated with lymphocyte differentiation and activation (e.g., lymphocyte chemotaxis, T-cell activation, and immunoglobulin secretion), as well as antigen-presenting cell functions, were shown to be differentially regulated in response to BKD. The infection level-responsive transcripts were related to several mechanisms such as the JAK-STAT signalling pathway, B-cell differentiation and interleukin-1 responses. Sixty-five microarray-identified transcripts were subjected to qPCR validation, and they showed the same fold-change direction as microarray results. The qPCR-validated transcripts studied herein play putative roles in various immune processes including pathogen recognition (e.g., tlr5), antibacterial activity (e.g., hamp and camp), ... Article in Journal/Newspaper Atlantic salmon Salmo salar Universidad Andrés Bello: Repositorio Institucional Académico Frontiers in Immunology 11 |
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Universidad Andrés Bello: Repositorio Institucional Académico |
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language |
English |
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responses immune response infection |
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responses immune response infection Eslamloo, K. Caballero-Solares, A. Inkpen, S.M. Emam, M. Kumar, S. Bouniot, C. Avendaño-Herrera, R. Jakob, E. Rise, M.L. Transcriptomic Profiling of the Adaptive and Innate Immune Responses of Atlantic Salmon to Renibacterium salmoninarum Infection |
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responses immune response infection |
description |
Indexación: Scopus. Bacterial Kidney Disease (BKD), which is caused by a Gram-positive, intracellular bacterial pathogen (Renibacterium salmoninarum), affects salmonids including Atlantic salmon (Salmo salar). However, the transcriptome response of Atlantic salmon to BKD remained unknown before the current study. We used a 44K salmonid microarray platform to characterise the global gene expression response of Atlantic salmon to BKD. Fish (~54 g) were injected with a dose of R. salmoninarum (H-2 strain, 2 × 108 CFU per fish) or sterile medium (control), and then head kidney samples were collected at 13 days post-infection/injection (dpi). Firstly, infection levels of individuals were determined through quantifying the R. salmoninarum level by RNA-based TaqMan qPCR assays. Thereafter, based on the qPCR results for infection level, fish (n = 5) that showed no (control), higher (H-BKD), or lower (L-BKD) infection level at 13 dpi were subjected to microarray analyses. We identified 6,766 and 7,729 differentially expressed probes in the H-BKD and L-BKD groups, respectively. There were 357 probes responsive to the infection level (H-BKD vs. L-BKD). Several adaptive and innate immune processes were dysregulated in R. salmoninarum-infected Atlantic salmon. Adaptive immune pathways associated with lymphocyte differentiation and activation (e.g., lymphocyte chemotaxis, T-cell activation, and immunoglobulin secretion), as well as antigen-presenting cell functions, were shown to be differentially regulated in response to BKD. The infection level-responsive transcripts were related to several mechanisms such as the JAK-STAT signalling pathway, B-cell differentiation and interleukin-1 responses. Sixty-five microarray-identified transcripts were subjected to qPCR validation, and they showed the same fold-change direction as microarray results. The qPCR-validated transcripts studied herein play putative roles in various immune processes including pathogen recognition (e.g., tlr5), antibacterial activity (e.g., hamp and camp), ... |
format |
Article in Journal/Newspaper |
author |
Eslamloo, K. Caballero-Solares, A. Inkpen, S.M. Emam, M. Kumar, S. Bouniot, C. Avendaño-Herrera, R. Jakob, E. Rise, M.L. |
author_facet |
Eslamloo, K. Caballero-Solares, A. Inkpen, S.M. Emam, M. Kumar, S. Bouniot, C. Avendaño-Herrera, R. Jakob, E. Rise, M.L. |
author_sort |
Eslamloo, K. |
title |
Transcriptomic Profiling of the Adaptive and Innate Immune Responses of Atlantic Salmon to Renibacterium salmoninarum Infection |
title_short |
Transcriptomic Profiling of the Adaptive and Innate Immune Responses of Atlantic Salmon to Renibacterium salmoninarum Infection |
title_full |
Transcriptomic Profiling of the Adaptive and Innate Immune Responses of Atlantic Salmon to Renibacterium salmoninarum Infection |
title_fullStr |
Transcriptomic Profiling of the Adaptive and Innate Immune Responses of Atlantic Salmon to Renibacterium salmoninarum Infection |
title_full_unstemmed |
Transcriptomic Profiling of the Adaptive and Innate Immune Responses of Atlantic Salmon to Renibacterium salmoninarum Infection |
title_sort |
transcriptomic profiling of the adaptive and innate immune responses of atlantic salmon to renibacterium salmoninarum infection |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
http://repositorio.unab.cl/xmlui/handle/ria/19400 https://doi.org/10.3389/fimmu.2020.567838 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Frontiers in ImmunologyOpen AccessVolume 1128 October 2020 Article number 567838 1664-3224 http://repositorio.unab.cl/xmlui/handle/ria/19400 doi:10.3389/fimmu.2020.567838 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ Attribution 4.0 International (CC BY 4.0) |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2020.567838 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
_version_ |
1766360696958746624 |