Transcriptomic response of Trematomus bernacchii to a short to mid term mild heat stress and bias of experimental design
Many stenotherm marine species live in the cold and stable environment of the Antarctic Ocean which could be impacted by climate change in the upcoming years. To investigate this issue, gene expression analysis was carried out on Trematomus bernacchii specimens caught near Mario Zucchelli Station. B...
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ftunitriestiris:oai:arts.units.it:11368/3020488 2023-05-15T13:44:28+02:00 Transcriptomic response of Trematomus bernacchii to a short to mid term mild heat stress and bias of experimental design Samuele Greco Serena Gaetano Anastasia Alberto Pallavicini Piero Giulio Giulianini Marco Gerdol Greco, Samuele Gaetano, ANASTASIA SERENA Pallavicini, Alberto Giulianini, PIERO GIULIO Gerdol, Marco 2021 http://hdl.handle.net/11368/3020488 http://www.uzionlus.it/documenti/elenco_abstract_allegato.pdf eng ita eng ita UZI ispartofbook:WEBINAR GIORNATE INTERDISCIPLINARI DI ZOOLOGIA 2021 SIMPOSIO “GIOVANI RICERCATORI UZI” ELENCO CONTRIBUTI UZI - GIORNATE INTERDISCIPLINARI DI ZOOLOGIA 2021 firstpage:11 lastpage:11 numberofpages:1 http://hdl.handle.net/11368/3020488 http://www.uzionlus.it/documenti/elenco_abstract_allegato.pdf info:eu-repo/semantics/openAccess Trematomus bernacchii transcriptomic Antarctica climate change info:eu-repo/semantics/conferenceObject 2021 ftunitriestiris 2023-04-09T06:21:32Z Many stenotherm marine species live in the cold and stable environment of the Antarctic Ocean which could be impacted by climate change in the upcoming years. To investigate this issue, gene expression analysis was carried out on Trematomus bernacchii specimens caught near Mario Zucchelli Station. Brain, gill and muscle tissues were sampled from naïve animals and from those kept in control (- 0.9°C) and experimental (+0.6°C) tanks for six hours, seven and twenty days, post acclimation. RNAseq data showed that the temperature increase mostly affected the brain, with a time dependent response. Immune response was up-regulated at mid-term; protein turnover and energy management were down-regulated and neuronal remodelling related genes were up-regulated at 20 days, where the highest number of differentially expressed genes was observed. Gill tissue showed a mild response after 20 days, mostly involving DNA replication. A great early-starting response to stabling was also observed across the entire experiment in brain and gills, with the latter being the most affected. The expression pattern clustering analysis showed that many synapse-related genes were down-regulated and energy related genes were up-regulated, while several binding processes were up-regulated at the latest time point in brain. The response in gills was milder and mostly involved cytoskeleton and glycolysis. No significant change was observed in muscle. These results identify the brain as the tissue most affected by both heat and stabling, evidencing its great sensitivity to the slightest environmental change, and highlight the importance of a careful experimental design when working with captive wild organisms. Conference Object Antarc* Antarctic Antarctic Ocean Antarctica Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) Antarctic Antarctic Ocean Mario Zucchelli ENVELOPE(164.123,164.123,-74.695,-74.695) Mario Zucchelli Station ENVELOPE(164.117,164.117,-74.700,-74.700) The Antarctic |
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Open Polar |
collection |
Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
op_collection_id |
ftunitriestiris |
language |
English Italian |
topic |
Trematomus bernacchii transcriptomic Antarctica climate change |
spellingShingle |
Trematomus bernacchii transcriptomic Antarctica climate change Samuele Greco Serena Gaetano Anastasia Alberto Pallavicini Piero Giulio Giulianini Marco Gerdol Transcriptomic response of Trematomus bernacchii to a short to mid term mild heat stress and bias of experimental design |
topic_facet |
Trematomus bernacchii transcriptomic Antarctica climate change |
description |
Many stenotherm marine species live in the cold and stable environment of the Antarctic Ocean which could be impacted by climate change in the upcoming years. To investigate this issue, gene expression analysis was carried out on Trematomus bernacchii specimens caught near Mario Zucchelli Station. Brain, gill and muscle tissues were sampled from naïve animals and from those kept in control (- 0.9°C) and experimental (+0.6°C) tanks for six hours, seven and twenty days, post acclimation. RNAseq data showed that the temperature increase mostly affected the brain, with a time dependent response. Immune response was up-regulated at mid-term; protein turnover and energy management were down-regulated and neuronal remodelling related genes were up-regulated at 20 days, where the highest number of differentially expressed genes was observed. Gill tissue showed a mild response after 20 days, mostly involving DNA replication. A great early-starting response to stabling was also observed across the entire experiment in brain and gills, with the latter being the most affected. The expression pattern clustering analysis showed that many synapse-related genes were down-regulated and energy related genes were up-regulated, while several binding processes were up-regulated at the latest time point in brain. The response in gills was milder and mostly involved cytoskeleton and glycolysis. No significant change was observed in muscle. These results identify the brain as the tissue most affected by both heat and stabling, evidencing its great sensitivity to the slightest environmental change, and highlight the importance of a careful experimental design when working with captive wild organisms. |
author2 |
Greco, Samuele Gaetano, ANASTASIA SERENA Pallavicini, Alberto Giulianini, PIERO GIULIO Gerdol, Marco |
format |
Conference Object |
author |
Samuele Greco Serena Gaetano Anastasia Alberto Pallavicini Piero Giulio Giulianini Marco Gerdol |
author_facet |
Samuele Greco Serena Gaetano Anastasia Alberto Pallavicini Piero Giulio Giulianini Marco Gerdol |
author_sort |
Samuele Greco |
title |
Transcriptomic response of Trematomus bernacchii to a short to mid term mild heat stress and bias of experimental design |
title_short |
Transcriptomic response of Trematomus bernacchii to a short to mid term mild heat stress and bias of experimental design |
title_full |
Transcriptomic response of Trematomus bernacchii to a short to mid term mild heat stress and bias of experimental design |
title_fullStr |
Transcriptomic response of Trematomus bernacchii to a short to mid term mild heat stress and bias of experimental design |
title_full_unstemmed |
Transcriptomic response of Trematomus bernacchii to a short to mid term mild heat stress and bias of experimental design |
title_sort |
transcriptomic response of trematomus bernacchii to a short to mid term mild heat stress and bias of experimental design |
publisher |
UZI |
publishDate |
2021 |
url |
http://hdl.handle.net/11368/3020488 http://www.uzionlus.it/documenti/elenco_abstract_allegato.pdf |
long_lat |
ENVELOPE(164.123,164.123,-74.695,-74.695) ENVELOPE(164.117,164.117,-74.700,-74.700) |
geographic |
Antarctic Antarctic Ocean Mario Zucchelli Mario Zucchelli Station The Antarctic |
geographic_facet |
Antarctic Antarctic Ocean Mario Zucchelli Mario Zucchelli Station The Antarctic |
genre |
Antarc* Antarctic Antarctic Ocean Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Ocean Antarctica |
op_relation |
ispartofbook:WEBINAR GIORNATE INTERDISCIPLINARI DI ZOOLOGIA 2021 SIMPOSIO “GIOVANI RICERCATORI UZI” ELENCO CONTRIBUTI UZI - GIORNATE INTERDISCIPLINARI DI ZOOLOGIA 2021 firstpage:11 lastpage:11 numberofpages:1 http://hdl.handle.net/11368/3020488 http://www.uzionlus.it/documenti/elenco_abstract_allegato.pdf |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766202093678362624 |