A new class of transparent organic particles in seawater visualized by a novel fluorescence approach
A method for visualizing transparent material in seawater, described here, has led to the discovery of novel particles. The protocol is based on Alcian Blue and SYBR Gold staining of seawater samples on polycarbonate filters. While the particles detected by our method may have some overlap with prev...
Published in: | Aquatic Microbial Ecology |
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Language: | English |
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2008
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Online Access: | http://hdl.handle.net/11368/2959748 https://doi.org/10.3354/ame01251 |
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ftunitriestiris:oai:arts.units.it:11368/2959748 2023-05-15T13:42:14+02:00 A new class of transparent organic particles in seawater visualized by a novel fluorescence approach Samo TJ Malfatti F Azam F Samo, Tj Malfatti, F Azam, F 2008 http://hdl.handle.net/11368/2959748 https://doi.org/10.3354/ame01251 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000262683300006 volume:53 issue:3 firstpage:307 lastpage:321 numberofpages:15 journal:AQUATIC MICROBIAL ECOLOGY http://hdl.handle.net/11368/2959748 doi:10.3354/ame01251 info:eu-repo/semantics/article 2008 ftunitriestiris https://doi.org/10.3354/ame01251 2023-04-09T06:18:01Z A method for visualizing transparent material in seawater, described here, has led to the discovery of novel particles. The protocol is based on Alcian Blue and SYBR Gold staining of seawater samples on polycarbonate filters. While the particles detected by our method may have some overlap with previously described transparent exopolymer particles and Coomassie stained particles, these particles largely comprise a previously undetected class. We propose that the particles are detected because they cause spatially explicit inhibition of Alcian Blue quenching of SYBR Cold fluorescence of the filter. Samples collected from various locations (Ellen Browning Scripps Memorial Pier, California, the Palmer Peninsula, Antarctica, and Point Conception, California) revealed particles with abundances on the order of 10 and 10(5) 1(-1) and ranging in size from 10 to 10(5) mu m(2). The particles varied in the types of organisms attached, the internal structure and probable biological sources. Field observations and laboratory experimental manipulations suggest varied sources and mechanisms of formation. These particles are potential hot spots of organic matter, microbial diversify and interactions, and, depending on their size and sinking rates, serve as conduits for carbon export to the ocean's interior. Article in Journal/Newspaper Antarc* Antarctica Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) Browning ENVELOPE(164.050,164.050,-74.617,-74.617) Scripps ENVELOPE(-63.783,-63.783,-69.150,-69.150) Aquatic Microbial Ecology 53 307 321 |
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Open Polar |
collection |
Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
op_collection_id |
ftunitriestiris |
language |
English |
description |
A method for visualizing transparent material in seawater, described here, has led to the discovery of novel particles. The protocol is based on Alcian Blue and SYBR Gold staining of seawater samples on polycarbonate filters. While the particles detected by our method may have some overlap with previously described transparent exopolymer particles and Coomassie stained particles, these particles largely comprise a previously undetected class. We propose that the particles are detected because they cause spatially explicit inhibition of Alcian Blue quenching of SYBR Cold fluorescence of the filter. Samples collected from various locations (Ellen Browning Scripps Memorial Pier, California, the Palmer Peninsula, Antarctica, and Point Conception, California) revealed particles with abundances on the order of 10 and 10(5) 1(-1) and ranging in size from 10 to 10(5) mu m(2). The particles varied in the types of organisms attached, the internal structure and probable biological sources. Field observations and laboratory experimental manipulations suggest varied sources and mechanisms of formation. These particles are potential hot spots of organic matter, microbial diversify and interactions, and, depending on their size and sinking rates, serve as conduits for carbon export to the ocean's interior. |
author2 |
Samo, Tj Malfatti, F Azam, F |
format |
Article in Journal/Newspaper |
author |
Samo TJ Malfatti F Azam F |
spellingShingle |
Samo TJ Malfatti F Azam F A new class of transparent organic particles in seawater visualized by a novel fluorescence approach |
author_facet |
Samo TJ Malfatti F Azam F |
author_sort |
Samo TJ |
title |
A new class of transparent organic particles in seawater visualized by a novel fluorescence approach |
title_short |
A new class of transparent organic particles in seawater visualized by a novel fluorescence approach |
title_full |
A new class of transparent organic particles in seawater visualized by a novel fluorescence approach |
title_fullStr |
A new class of transparent organic particles in seawater visualized by a novel fluorescence approach |
title_full_unstemmed |
A new class of transparent organic particles in seawater visualized by a novel fluorescence approach |
title_sort |
new class of transparent organic particles in seawater visualized by a novel fluorescence approach |
publishDate |
2008 |
url |
http://hdl.handle.net/11368/2959748 https://doi.org/10.3354/ame01251 |
long_lat |
ENVELOPE(164.050,164.050,-74.617,-74.617) ENVELOPE(-63.783,-63.783,-69.150,-69.150) |
geographic |
Browning Scripps |
geographic_facet |
Browning Scripps |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000262683300006 volume:53 issue:3 firstpage:307 lastpage:321 numberofpages:15 journal:AQUATIC MICROBIAL ECOLOGY http://hdl.handle.net/11368/2959748 doi:10.3354/ame01251 |
op_doi |
https://doi.org/10.3354/ame01251 |
container_title |
Aquatic Microbial Ecology |
container_volume |
53 |
container_start_page |
307 |
op_container_end_page |
321 |
_version_ |
1766165774144110592 |