Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2...
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ftunitriestiris:oai:arts.units.it:11368/2957222 2023-05-15T14:13:52+02:00 Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus Gerdol, Marco Lucente, Daniela Buonocore, Francesco Poerio, Elia Scapigliati, Giuseppe Mattiucci, Simonetta Pallavicini, Alberto Cimmaruta, Roberta Gerdol, Marco Lucente, Daniela Buonocore, Francesco Poerio, Elia Scapigliati, Giuseppe Mattiucci, Simonetta Pallavicini, Alberto Cimmaruta, Roberta 2019 http://hdl.handle.net/11368/2957222 https://doi.org/10.1038/s41598-019-42003-5 https://www.nature.com/articles/s41598-019-42003-5 eng eng info:eu-repo/semantics/altIdentifier/pmid/30940855 info:eu-repo/semantics/altIdentifier/wos/WOS:000462990000065 volume:9 issue:1 firstpage:5523 lastpage:5523 numberofpages:1 journal:SCIENTIFIC REPORTS http://hdl.handle.net/11368/2957222 doi:10.1038/s41598-019-42003-5 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85063891801 https://www.nature.com/articles/s41598-019-42003-5 info:eu-repo/semantics/openAccess icefish Antarctica MHC evolution cold adaptation info:eu-repo/semantics/article 2019 ftunitriestiris https://doi.org/10.1038/s41598-019-42003-5 2023-04-09T06:17:36Z This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2 sequence of the highly expressed gene ChhaDAB has been observed, resulting in an estimate of 92 different variants translated in 87 different peptides from 54 analysed icefish individuals. A highly conservative estimate, based on a 95% sequence identity threshold clustering, translate this variability in 41 different peptide clusters belonging to four different clades and showing the signature of different kinds of selection. In stark contrast, the poorly expressed ChhaDBB gene displayed a very low level of molecular diversity within exon 2, in agreement with expectations for a nonclassical MHC class II β gene. Article in Journal/Newspaper Antarc* Antarctic Antarctica Icefish Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) Antarctic The Antarctic Scientific Reports 9 1 |
institution |
Open Polar |
collection |
Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
op_collection_id |
ftunitriestiris |
language |
English |
topic |
icefish Antarctica MHC evolution cold adaptation |
spellingShingle |
icefish Antarctica MHC evolution cold adaptation Gerdol, Marco Lucente, Daniela Buonocore, Francesco Poerio, Elia Scapigliati, Giuseppe Mattiucci, Simonetta Pallavicini, Alberto Cimmaruta, Roberta Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
topic_facet |
icefish Antarctica MHC evolution cold adaptation |
description |
This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2 sequence of the highly expressed gene ChhaDAB has been observed, resulting in an estimate of 92 different variants translated in 87 different peptides from 54 analysed icefish individuals. A highly conservative estimate, based on a 95% sequence identity threshold clustering, translate this variability in 41 different peptide clusters belonging to four different clades and showing the signature of different kinds of selection. In stark contrast, the poorly expressed ChhaDBB gene displayed a very low level of molecular diversity within exon 2, in agreement with expectations for a nonclassical MHC class II β gene. |
author2 |
Gerdol, Marco Lucente, Daniela Buonocore, Francesco Poerio, Elia Scapigliati, Giuseppe Mattiucci, Simonetta Pallavicini, Alberto Cimmaruta, Roberta |
format |
Article in Journal/Newspaper |
author |
Gerdol, Marco Lucente, Daniela Buonocore, Francesco Poerio, Elia Scapigliati, Giuseppe Mattiucci, Simonetta Pallavicini, Alberto Cimmaruta, Roberta |
author_facet |
Gerdol, Marco Lucente, Daniela Buonocore, Francesco Poerio, Elia Scapigliati, Giuseppe Mattiucci, Simonetta Pallavicini, Alberto Cimmaruta, Roberta |
author_sort |
Gerdol, Marco |
title |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_short |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_full |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_fullStr |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_full_unstemmed |
Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus |
title_sort |
molecular and structural characterization of mhc class ii β genes reveals high diversity in the cold-adapted icefish chionodraco hamatus |
publishDate |
2019 |
url |
http://hdl.handle.net/11368/2957222 https://doi.org/10.1038/s41598-019-42003-5 https://www.nature.com/articles/s41598-019-42003-5 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica Icefish |
genre_facet |
Antarc* Antarctic Antarctica Icefish |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/30940855 info:eu-repo/semantics/altIdentifier/wos/WOS:000462990000065 volume:9 issue:1 firstpage:5523 lastpage:5523 numberofpages:1 journal:SCIENTIFIC REPORTS http://hdl.handle.net/11368/2957222 doi:10.1038/s41598-019-42003-5 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85063891801 https://www.nature.com/articles/s41598-019-42003-5 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/s41598-019-42003-5 |
container_title |
Scientific Reports |
container_volume |
9 |
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1 |
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1766286394120994816 |